Copper induction of laccases by Lentinus polychrous under liquid-state fermentation

Khammuang, Saranyu; Yuwa-amornpitak, Thalisa; Svasti, Jisnuson; Sarnthima, Rakrudee

doi:10.1016/j.bcab.2013.05.004

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…1). Laccase activity clearly dropped when using 2 mM CuSO 4 indicating that toxicity of copper ions to the fungus as discussed in a previous report 12 . Laccase production by liquid culture in 250 mL-Erlenmeyer flasks did not has enough laccase activity to study rice straw pretreatment.…”

Section: Laccase Production By L Polychrous In Liquid and Solid Mediumsupporting

confidence: 64%

Laccase from Lentinus polychrous Increases the Efficiency of Cellulose Hydrolysis of Rice Straw to Glucose

Sarnthima¹,

Khammuang²

2018

Orient. J. Chem

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This study investigated the pretreatment of rice straw by crude laccase from Lentinus polychrous for preparation of lignocellulosic materials prior to be hydrolysed with cellulase for obtaining reducing sugar. Production of laccase involved cultivation of L. polychrous on solid media containing rice bran and rice husk supplemented with copper sulfate Pretreatment the rice straw was performed by incubating laccase (2, 5, 10 U) with 10 g of rice straw in buffer at 40 and 50 o C for 24 hours. Then the rice straw was analyzed by fourier transform infrared spectroscopy (FT-IR). Laccase-pretreated rice straws were further hydrolyzed by commercial cellulase from Trichoderma reesei at activity of 0, 10, 20, 30 Filter Paper Unit (FPU) for 24 hours. Then reducing sugar was extracted and determined by the dinitrosalicylic acid (DNS) method. It was found that the rice straw pretreated with crude laccase gave a higher level of reducing sugar than that of crude laccase pretreatment. Rice straw hydrolyzed by 2 U of laccase at 40 and 50 o C yielded the highest reducing sugar at every cellulase activity used (10, 20 and 30 FPU). The results showed that crude laccase could be applied to increase the efficiency of lignocellulose hydrolysis by cellulase.

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Section: Laccase Production By L Polychrous In Liquid and Solid Mediumsupporting

confidence: 64%

Laccase from Lentinus polychrous Increases the Efficiency of Cellulose Hydrolysis of Rice Straw to Glucose

Sarnthima¹,

Khammuang²

2018

Orient. J. Chem

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“…Fonseca et al 43 produced the highest laccase activity at 0.5 mM Cu 2+ in culture medium of G. applanatum and Peniophora sp. The enzyme production and fungal growth was inhibited under the higher concentration of Cu 2+ (3 mM) 95 . The optimal concentrations of Cu 2+ were 0.1 and 0.5 mM enhanced the laccase from P. ostreatus and Streptomyces lavendulae 96 , respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The optimal concentrations of Cu 2+ were 0.1 and 0.5 mM enhanced the laccase from P. ostreatus and Streptomyces lavendulae 96 , respectively. In fungal organisms, copper ions are essential to form the intact and active structures of laccase, whereas many reports showed that copper ions badly affect the laccase production ability 95 . Copper induction also influences the genetic transcription level of laccase 96 , 97 .…”

Section: Discussionmentioning

confidence: 99%

Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship

Umar

Ahmed

2022

Sci Rep

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The aim of this work to study an efficient laccase producing fungus Ganoderma leucocontextum, which was identified by ITS regions of DNA and phylogenetic tree was constructed. This study showed the laccase first-time from G. leucocontextum by using medium containing guaiacol. The growth cultural (pH, temperature, incubation days, rpm) and nutritional (carbon and nitrogen sources) conditions were optimized, which enhanced the enzyme production up to 4.5-folds. Laccase production increased 855 U/L at 40 °C. The pH 5.0 was suitable for laccase secretion (2517 U/L) on the 7th day of incubation at 100 rpm (698.3 U/L). Glucose and sucrose were good carbon source to enhance the laccase synthesis. The 10 g/L beef (4671 U/L) and yeast extract (5776 U/L) were the best nitrogen source for laccase secretion from G. leucocontextum. The laccase was purified from the 80% ammonium sulphate precipitations of protein identified by nucleotides sequence. The molecular weight (65.0 kDa) of purified laccase was identified through SDS and native PAGE entitled as Glacc110. The Glacc110 was characterized under different parameters. It retained > 90% of its activity for 16 min incubation at 60 °C in acidic medium (pH 4.0). This enzyme exerted its optimal activity at pH 3.0 and temperature 70 °C with guaiacol substrate. The catalytic parameters Km and Vmax was 1.658 (mM) and 2.452 (mM/min), respectively. The thermo stability of the laccase produced by submerged fermentation of G. leucocontextum has potential for industrial and biotechnology applications. The results remarked the G. leucocontextum is a good source for laccase production.

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“…Increase in the production or activity of an extracellular enzyme such as laccase has been observed in the presence of essential metals such as Cu, Fe, Mn, Co and Zn. For instance, CuSO 4 at 0.5-1 mM concentration increased the production of laccases from Pleurotus ostreatus (Baldrian and Gabriel, 2002;Bhattacharya et al, 2014) and Lentinus polychrous (Khammuang et al, 2013). Enzymes are strongly regulated at the transcription level.…”

Section: Impacts On Enzyme Secretion Capacitymentioning

confidence: 99%

Impact of wastewater derived dissolved interfering compounds on growth, enzymatic activity and trace organic contaminant removal of white rot fungi – A critical review

Asif

Hai

Hou

et al. 2017

Journal of Environmental Management

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White-rot fungi (WRF) and their ligninolytic enzymes have been investigated for the removal of a broad spectrum of trace organic contaminants (TrOCs) mostly from synthetic wastewater in lab-scale experiments. Only a few studies have reported the efficiency of such systems for the removal of TrOCs from real wastewater. Wastewater derived organic and inorganic compounds can inhibit: (i) WRF growth and their enzyme production capacity; (ii) enzymatic activity of ligninolytic enzymes; and (iii) catalytic efficiency of both WRF and enzymes. It is observed that essential metals such as Cu, Mn and Co at trace concertation (up to 1 mM) can improve the growth of WRF species, whereas non-essential metal such as Pb, Cd and Hg at 1 mM concentration can inhibit WRF growth and their enzyme production. In the case of purified enzymes, most of the tested metals at 1-5 mM concentration do not significantly inhibit the activity of laccases. Organic interfering compounds such as oxalic acid and ethylenediaminetetraacetic acid (EDTA) at 1 mM concentration are potent inhibitors of WRF and their extracellular enzymes. However, inhibitory effects induced by interfering compounds are strongly influenced by the type of WRF species as well as experimental conditions (e.g., incubation time and TrOC type). In this review, mechanisms and factors governing the interactions of interfering compounds with WRF and their ligninolytic enzymes are reviewed and elucidated. In addition, the performance of WRF and their ligninolytic enzymes for the removal of TrOCs from synthetic and real wastewater is critically summarized.

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Copper induction of laccases by Lentinus polychrous under liquid-state fermentation

Cited by 9 publications

References 31 publications

Laccase from Lentinus polychrous Increases the Efficiency of Cellulose Hydrolysis of Rice Straw to Glucose

Laccase from Lentinus polychrous Increases the Efficiency of Cellulose Hydrolysis of Rice Straw to Glucose

Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship

Impact of wastewater derived dissolved interfering compounds on growth, enzymatic activity and trace organic contaminant removal of white rot fungi – A critical review

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