To study lysosomes with an ultralong term, herein, noble-metal
Au and Ag nanoclusters (MNCs) modified with biocompatible protein
bovine serum albumin (BSA) called BSA–NCs were
applied to image lysosomes under structured illumination microscopy
(SIM). Compared with commercial lysosomal dyes, BSA–NCs exhibited remarkable anti-interference in pH fluctuation, excellent
antiphotobleaching for ultralong-term lysosomal tracking, and impressive
near-infrared fluorescence. The lysosomal events (i.e., fission, fusion,
and kiss-and-run events) were observed with the BSA–NCs under SIM, as was the interplay between lysosomes and mitochondria
leading to mitophagy. With their molecular-like properties and near-infrared
fluorescence, BSA–Au NCs proved able to enter
the internal cells of organoids and, for the first time, were applied
to track subcellular lysosomal dynamics. The results not only demonstrate
that MNCs are excellent tools for imaging organelles in living cells
and organoids with super-resolution technology but also highlight
new fields for using MNCs in ultralong-term bioimaging.