Copper removal ability byStreptomycesstrains with dissimilar growth patterns and endowed with cupric reductase activity

Abstract

Morphological, physiological and molecular characterization of three copper-resistant actinobacterial strains (AB2A, AB3 and AB5A) isolated from copper-polluted sediments of a drainage channel showed that they belonged to the genus Streptomyces. These characteristics plus their distinctive copper resistance phenotypes revealed considerable divergence among the isolates. Highly dissimilar growth patterns and copper removal efficiency were observed for the selected Streptomyces strains grown on minimal medium (MM) added with 0.5 mM of copper sulfate (MM(Cu)). Strain AB2A showed an early mechanism of copper uptake/retention (80% until day 3), followed by a drastic metal efflux process (days 5-7). In contrast, Streptomyces sp. AB3 and AB5A showed only copper retention phenotypes under the same culture conditions. Particularly, Streptomyces sp. AB5A showed a better efficiency in copper removal (94%), although a longer lag phase was observed for this microorganism grown for 7 days in MM(Cu). Cupric reductase activity was detected in both copper-adapted cells and nonadapted cells of all three strains but this activity was up to 100-fold higher in preadapted cells of Streptomyces sp. AB2A. To our knowledge, this is the first time that cupric reductase activity was demonstrated in Streptomyces strains.

­Genomic data mining of the marine actinobacteriaStreptomycessp. H-KF8 unveils insights into multi-stress related genes and metabolic pathways involved in antimicrobial synthesis

“…Environmental pollution by heavy metals can arise due to anthropogenic and/or geogenic sources. Although metal-resistant strains isolated from contaminated areas have been described ( Amoroso et al, 2001 ; Schmidt et al, 2005 ; Schmidt et al, 2009 ; Polti, Amoroso & Abate, 2007 ; Albarracin et al, 2008 ; Haferburg et al, 2008 ; Siñeriz, Kothe & Abate, 2009 ; Lin et al, 2011 ; El Baz et al, 2015 ), there is limited information about the physiology of Streptomyces in presence of environmental metal pollutants. Due to the naturally high concentrations of certain heavy metals in Chilean northern Patagonia ( Guevara et al, 2004 ; Revenga et al, 2012 ; Hermanns & Biester, 2013 ) product of the highly active seismic and volcanic activity ( Pantoja, Luis Iriarte & Daneri, 2011 ), the ability of Streptomyces sp.…”

Simultaneous bioremediation of Cr(VI) and lindane in soil by actinobacteria

“…M7, A2, A5, and A11, isolated from sediments and soils contaminated with OPs, were found to be able to degrade lindane, as revealed by the release of chloride ions when the microorganisms were grown on media containing this pesticide as a sole carbon source (Benimeli et al, 2003(Benimeli et al, , 2006Cuozzo et al, 2009;Fuentes et al, 2010). Biotransformation of heavy metals [Cu (II), Cd (II), and Cr (VI)] by actinobacteria, particularly in terms of uptake and/or reduction to less toxic forms, has also been studied (Polti et al, 2007;Albarracín et al, 2008a;Siñeriz et al, 2009). Streptomyces sp.…”

Resistance to Heavy Metals of Actinobacteria Isolated From the Biological Fouling of Shellfish and Mussels of the Odesa Gulf of the Black Sea

“…У дослідженнях інших авторів показано, що реакція мікроорганізмів різних таксономічних груп на купрум (як і на багато інших важких металів) визначається багатьма параметрами і залежить, зокрема, від виду мікроорганізму (а часто навіть штаму), джерела виділення, концентрації металу, умов проведення експерименту та ін. [2,3,8]. Так, у дослідженнях Н.Ю.…”

“…Штами Streptomyces spp. AB2A, AB3 і AB5A, виділені із забруднених купрумом відкладень дренажного каналу, інтенсивно росли на мінімальному середовищі із 0,5 мM вмістом сульфату купруму, при цьому демонструючи морфологічну, фізіологічну і молекулярну неоднорідність [8]. При формуванні стійкості до купруму у актинобактерій відзначають механізми біоакумуляції, біосорбції та біотрансформації [19].…”