Copper resistance of different ectomycorrhizal fungi such as Pisolithus microcarpus, Pisolithus sp., Scleroderma sp. and Suillus sp.

Abstract

Environments contaminated with heavy metals negatively impact the living organisms. Ectomycorrhizal fungi have shown important role in these impacted sites. Thus, this study aimed to evaluate the copper-resistance of ectomycorrhizal fungi isolates Pisolithus microcarpus - UFSC-Pt116; Pisolithus sp. - UFSC-PT24, Suillus sp. - UFSM RA 2.8 and Scleroderma sp. - UFSC-Sc124 to different copper doses in solid and liquid media. The copper doses tested were: 0.00, 0.25, 0.5, 0.75, 1.0 and 1.25 mmol L−1 in the solid medium and 0.00, 0.32, 0.64 and 0.96 mmol L−1 in the liquid medium. Copper was amended as copper sulphate in order to supplement the culture medium MNM at pH 4.8, with seven replicates to each fungus-dose combination. The fungal isolates were incubated for 30 days at 28 °C. UFSC-Pt116 showed high copper-resistance such as accessed by CL50 determinations (concentration to reduce 50% of the growth) as while as UFSC-PT24 displayed copper-resistance mechanism at 0.50 mmol L−1 in solid medium. The UFSC-PT24 and UFSC-Sc124 isolates have increased copper-resistance in liquid medium. The higher production of extracellular pigment was detected in UFSC-Pt116 cultures. The UFSC-Pt116 and UFSC-PT24 isolates showed higher resistance for copper and produced higher mycelium biomass than the other isolates. In this way, the isolates UFSG-Pt116 and UFSC-PT24 can be important candidates to survive in copper-contaminated areas, and can show important role in plants symbiosis in these contaminated sites.

Cellular Response to Cu‐ and Zn‐Induced Oxidative Stress in Aspergillus fumigatus Isolated From Polluted Soils in Bulgaria

“…For instance, Acremonium pinkertoniae , growing on agar media, also showed a lower tolerance to Cu ions in comparison to that demonstrated under submerged cultivation . Such difference in responses to metals has been indicated for various ectomycorrhizal fungi . The results of this study confirm these reports.…”

“…The higher tolerance in liquid medium can be attributed to the precipitation mechanism. Cultivation in this medium allows production of a different organic acid, which acts in the chelation mechanisms of heavy metals . The addition of glucose to the medium caused an enhanced metal tolerance for many fungal strains .…”

Seleção de fungicidas visando à preservação de fungos micorrízicos arbusculares nativos no cultivo do feijoeiro

“…Este resultado pode ser atribuído à alta capacidade residual desses produtos no solo e do seu baixo potencial de translocação (Andrei, 2009). Fungos ectomicorrízicos de espécies florestais também apresentaram redução no desenvolvimento à medida que as doses de cobre foram aumentadas indicando a inabilidade dessas espécies em crescer em ambiente com excesso deste elemento (Silva et al, 2013).…”

A novel, highly conserved metallothionein family in basidiomycete fungi and characterization of two representativeSlMTaandSlMTbgenes in the ectomycorrhizal fungusSuillus luteus

“…However, there is some evidence that particular wood-rot fungi became tolerant to the metal-containing A novel metallothionein family in basidiomycetes 2583 preservatives (Baldrian, 2003;Green Iii and Clausen, 2003;Hastrup et al, 2005;Guillen et al, 2009). Also ECM fungi from Cu-polluted soils may develop Cu resistance and such ecotypes may be good candidates for bioremediation of Cu-polluted areas Colpaert et al, 2011;Silva et al, 2013). Therefore, understanding how these fungi cope with excess Cu and heavy metals in general will help to develop new technologies for the control and efficient use of these fungi in the future.…”