Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis

Bu, Ru; Yan, Bing; Sun, Haijie; Zhou, Miaolei; Bai, Huashan; Cai, Xinghua; Mo, Xueyan; Su, Guijiao; Jiang, Chengjian

doi:10.3389/fmicb.2021.771878

Cited by 9 publications

(3 citation statements)

References 73 publications

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“…Amino acids metabolic, especially the metabolic of glutathione, cysteine, and methionine, were significantly up-regulated in the treatment of high concentrations of copper, which indicates that these metabolic pathways were responsible for P. spadix ZSY-33 to deal with higher copper stress. These results were consistent with studies in yeast Meyerozyma guilliermondii GXDK6, which reported that the metabolism of glutathione was elevated by the copper stress (Bu et al, 2021).…”

Section: Discussionsupporting

confidence: 92%

The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

Wang

Zhang

2023

Environ Microbiol Rep

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Resolving the heavy metal resistance mechanisms of microbes is crucial for understanding the bioremediation of the ecological environment. In this study, a multiple heavy metal resistance bacterium, Pseudoxanthomonas spadix ZSY‐33 was isolated and characterized. The copper resistance mechanism was revealed by analysis of the physiological traits, copper distribution, and genomic and transcriptomic data of strain ZSY‐33 cultured with different concentrations of copper. The growth inhibition assay in basic medium showed that the growth of strain ZSY‐33 was inhibited in the presence of 0.5 mM copper. The production of extracellular polymeric substances increased at a lower concentration of copper and decreased at a higher concentration of copper. Integrative analysis of genomic and transcriptomic, the copper resistance mechanism in strain ZSY‐33 was elucidated. At a lower concentration of copper, the Cus and Cop systems were responsible for the homeostasis of intracellular copper. As the concentration of copper increased, multiple metabolism pathways, including the metabolism of sulfur, amino acids, and pro‐energy were cooperated with the Cus and Cop systems to deal with copper stress. These results indicated a flexible copper resistance mechanism in strain ZSY‐33, which may acquire from the long‐term interaction with the living environment.

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Section: Discussionsupporting

confidence: 92%

The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

Wang

Zhang

2023

Environ Microbiol Rep

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“…Strains from M. guilliermondii have been isolated from a wide variety of environments [59], including deep-sea hydrothermal ridges [60] and dishwashers [61], [62], which are notably known to host extremophilic yeasts [61], [62]. This non-pigmented yeast is known to be tolerant to multiple stresses including salt (16% NaCl [63] and 18% KCl [64]), copper [65] and manganese (5494 ppm) [64]. It has moreover been widely used in biotechnological applications, as it is able to use various substrates for growth and to produce several useful compounds [66].…”

Section: Discussionmentioning

confidence: 99%

Co-isolation ofPenicillium citrinumand its cell-switching partnerMeyerozyma guilliermondiifrom a geothermal power plant

Bregnard,

Cailleau,

van Zonneveld

et al. 2024

Preprint

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Progresses in geothermal energy and deep drilling technologies have opened a new window into the terrestrial subsurface. This provides direct access to deep geothermal fluids used to produce heat and electricity, creating an opportunity to isolate and characterize novel microbial strains from these extreme habitats. In this study, we report the co-isolation of two fungal strains.Penicillium citrinum(strain HEK1) was isolated first and thought to be axenic. However, upon exposure to stress (frost and ethanol), a second strain, corresponding to the dimorphic yeastMeyerozyma guilliermondii(strain HEK2), appeared in HEK1 cultures. Strain HEK2 appeared first in the cultures and was followed by the subsequent re-growth of strain HEK1, underscoring their close relationship. Moreover, strain HEK2, able to switch from yeast cells to pseudohyphae when growing alone, did not produce pseudohyphae when in direct contact with strain HEK1. Altogether, our results indicate an intricate interaction between these strains that may allow them to thrive in the deep subsurface. These two fungi represent the first fungal strains isolated from deep geothermal fluids. Their presence within the fluids was confirmed through molecular analysis. The isolation of these strains emphasizes the importance of considering fungi when investigating microbial diversity in subsurface geothermal environments.Graphical abstractHighlightsFirst fungal strains isolated from a geothermal power plantThe two fungal strains were co-isolated from a geothermal fluid used for heat productionSurprising isolation of the cell-switching yeast upon stress exposure of an apparently axenic culture of the filamentous fungusFungal strains with high resistance to stressors and no apparent competition for carbon sources

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“…By MicrobioSee, users can plot a histogram easily with axis transposition and group sorting with one click. The data for this example are from the study of copper tolerance in Meyerozyma guilliermondii GXDK6, which was screened from the mangrove sediments (Bu et al, 2021). Multi-omics techniques were used to explore the tolerance mechanisms of the target strain with different copper ion concentrations.…”

Section: Casementioning

confidence: 99%

MicrobioSee: A Web-Based Visualization Toolkit for Multi-Omics of Microbiology

Sang

Zhang

et al. 2022

Front. Genet.

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With the upgrade and development of the high-throughput sequencing technology, multi-omics data can be obtained at a low cost. However, mapping tools that existed for microbial multi-omics data analysis cannot satisfy the needs of data description and result in high learning costs, complex dependencies, and high fees for researchers in experimental biology fields. Therefore, developing a toolkit for multi-omics data is essential for microbiologists to save effort. In this work, we developed MicrobioSee, a real-time interactive visualization tool based on web technologies, which could visualize microbial multi-omics data. It includes 17 modules surrounding the major omics data of microorganisms such as the transcriptome, metagenome, and proteome. With MicrobioSee, methods for plotting are simplified in multi-omics studies, such as visualization of diversity, ROC, and enrichment pathways for DEGs. Subsequently, three case studies were chosen to represent the functional application of MicrobioSee. Overall, we provided a concise toolkit along with user-friendly, time-saving, cross-platform, and source-opening for researchers, especially microbiologists without coding experience. MicrobioSee is freely available at https://microbiosee.gxu.edu.cn.

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Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis

Cited by 9 publications

References 73 publications

The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

Co-isolation ofPenicillium citrinumand its cell-switching partnerMeyerozyma guilliermondiifrom a geothermal power plant

MicrobioSee: A Web-Based Visualization Toolkit for Multi-Omics of Microbiology

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