Copper–zinc superoxide dismutase activity in healthy and inflamed human dental pulp

Abstract

These results demonstrate a potential role for Cu, Zn-SOD during dental pulp inflammation in humans.

Biochemical Indicators of Dental Caries in Saliva: An in vivo Study

“…SOD is a detoxifying antioxidant enzyme which acts against free radicals by catalyzing the dismutation of the superoxide ion into oxygen and hydrogen peroxide [Halliwell, 1999;Varvara et al, 2005]. This study showed an increase in SOD activity in saliva in caries-active adults when compared with that of caries-free individuals, suggesting its role as an antioxidant.…”

“…The estimation of SOD enzyme activity was carried out by the Beauchamp and Fridovich method [Green, 1970;Beauchamp and Fridovich, 1971;Gråhn et al, 1988;Varvara et al, 2005]. The substrate used for the assay consisted of nitro blue tetrazolium chloride, which reacts with superoxide anions produced upon illumination of riboflavin in the presence of methionine as an electron donor to produce formazan, which is a blue-colored complex.…”

“…mitochondria; and (3) extracellular SOD [Halliwell, 1999;Varvara et al, 2005]. The aim of this study was to evaluate SOD activity as well as copper and zinc levels in saliva of caries-active and caries-free individuals and to determine the correlation between SOD and copper and zinc levels.…”

Elevated expression of Cu, Zn‐SOD and Mn‐SOD mRNA in inflamed dental pulp tissue

“…Furthermore, different pH conditions were used in different studies of SOD enzyme activity, which can also explain the discrepancy found in enzyme activity and consequently the results of the studies (Davis et al. 1991, Marklund 1992, Varvara et al. 2005).…”

In vivo astaxanthin treatment partially prevents antioxidant alterations in dental pulp from alloxan‐induced diabetic rats

“…There are reports showing an increase in SOD in cases of irreversible pulpitis (Bodor et al. 2007) and reduction in its activity in irreversible symptomatic pulpitis tissue (Varvara et al. 2005).…”