2010
DOI: 10.1128/jb.01342-09
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Coproporphyrin Excretion and Low Thiol Levels Caused by Point Mutation in the Rhodobacter sphaeroides S -Adenosylmethionine Synthetase Gene

Abstract: A spontaneous mutant of Rhodobacter sphaeroides f. sp. denitrificans IL-106 was found to excrete a large amount of a red compound identified as coproporphyrin III, an intermediate in bacteriochlorophyll and heme synthesis. The mutant, named PORF, is able to grow under phototrophic conditions but has low levels of intracellular cysteine and glutathione and overexpresses the cysteine synthase CysK. The expression of molybdoenzymes such as dimethyl sulfoxide (DMSO) and nitrate reductases is also affected under ce… Show more

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Cited by 6 publications
(10 citation statements)
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“…A mutation in metK , the SAM synthetase‐coding gene, in R. sphaeroides f. sp. denitrificans , resulted in the excretion of coproporphyrin III (CPn) (Sabaty et al ., ), which corroborates the idea that SAM is required for this O 2 ‐independent CPO reaction. HemN, which belongs to the radical SAM superfamily (Sofia et al ., ; Layer et al ., ; Marsh et al ., ), contains a 4Fe‐4S cluster coordinated by a SAM molecule (henceforth denoted SAM1) and three closely spaced cysteine residues [C‐x(3)‐C‐x(2)‐C] (Layer et al ., 2003; 2004; Marsh et al ., ).…”
Section: Introductionsupporting
confidence: 85%
“…A mutation in metK , the SAM synthetase‐coding gene, in R. sphaeroides f. sp. denitrificans , resulted in the excretion of coproporphyrin III (CPn) (Sabaty et al ., ), which corroborates the idea that SAM is required for this O 2 ‐independent CPO reaction. HemN, which belongs to the radical SAM superfamily (Sofia et al ., ; Layer et al ., ; Marsh et al ., ), contains a 4Fe‐4S cluster coordinated by a SAM molecule (henceforth denoted SAM1) and three closely spaced cysteine residues [C‐x(3)‐C‐x(2)‐C] (Layer et al ., 2003; 2004; Marsh et al ., ).…”
Section: Introductionsupporting
confidence: 85%
“…A recent study described a spontaneous R. sphaeroides mutant called PORF, which excretes a large amount of coprophyrin III and has a mutation in the metK gene that encodes S-adenosylmethionine (SAM) synthetase (Sabaty et al, 2010). It was proposed that depletion of SAM decreased the SAM-dependent coproporphyrinogen oxidase activity, leading to a coproporphyrin III-accumulation phenotype.…”
Section: Resultsmentioning
confidence: 99%
“…Some mutants excrete a combination of coproporphyrin III and protoporphytin IX, lack c -type cytochromes, and cannot grow phototrophically (Biel and Biel, 1990; Goldman et al, 1997; Deshmukh et al, 2000), and these mutants differ from the R. capsulatus mutant SB1707 described here, which is capable of phototrophic growth. Others, such as the R. sphaeroides “PORF” ( metK ) mutant (Sabaty et al, 2010) and the R. gelatinosus copA mutant (Azzouzi et al, 2013), resemble strain SB1707 because they are capable of phototrophic growth. However, the rcc01707 gene differs from metK , which encodes S-adenosylmethionine (SAM) synthase, and addition of SAM or the SAM precursor methionine to the growth medium did not rescue the coproporphyrin III accumulation phenotype of SB1707.…”
Section: Discussionmentioning
confidence: 99%
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