CoQ10-loaded liposomes combined with UTMD prevented early nephropathy of diabetic rats

Abstract

Nephropathy is one of the most severe complications of diabetic patients. The therapeutic strategies for diabetic patients should not only focus on the control of blood glucose but also pay attention to the occurrence of diabetic nephropathy (DN). Coenzyme Q10 (CoQ10) has great therapeutic potential for DN. However, the clinical application of CoQ10 has been limited because of its low water-solubility and non-specific distribution. Liposomes were supposed to be an effective way for delivering CoQ10 to kidney. CoQ10 was effectively encapsulated into the liposome (CoQ10-LIP) with a high entrapment efficiency of 86.15 %. The CoQ10-LIP exhibited a small hydrodynamic diameter (180 ± 2.1 nm) and negative zeta potential (−18.20 mV). Moreover, CoQ10-LIP was combined with ultrasound-mediated microbubble destruction (UTMD) to enhance specific distribution of CoQ10 in kidney. In early stage of diabetic mellitus (DM), rats were administrated with CoQ10-LIP followed by UTMD (CoQ10-LIP+UTMD) to prevent occurrence of DN. Results revealed that CoQ10-LIP+UTMD effectively prevented the renal morphology and function of diabetics rats from damage. The protective mechanism of CoQ10-LIP was highly associated with protecting podocyte, promoting vascular repair and inhibiting cell apoptosis. Conclusively, CoQ10-LIP in combination with UTMD might be a potential strategy to prevent occurrence of DN.

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“… GIII: EFA groups received NQ (10 mg/kg body weight) IP daily for 7 days [8] . GIV: EFA groups received liposomal loaded NQ (10 mg/kg body weight) IP daily for 7 days [16] . GV: EFA groups were sacrificed post 14 days.…”

“…Liposomes have been extensively studied as drug carriers for several decades, because of their non-toxicity and non-immunogenicity. They can accommodate hydrophilic and lipophilic drugs and control the release of their encapsulated contents [16] . Thanks to their distinctive bilayer membrane structure, liposomes can cross the membrane and deliver NQ to the mitochondria [57] .…”

“…Thanks to their distinctive bilayer membrane structure, liposomes can cross the membrane and deliver NQ to the mitochondria [57] . In vivo and in vitro , liposomal encapsulation of NQ increased its water solubility and stability, which is a potential delivery method [16] .…”

Advancements in nanomedicines for the detection and treatment of diabetic kidney disease

“… [ 149 ] CoQ10-loaded liposomes 180 STZ-induced diabetic male Sprague-Dawley rats CoQ10-loaded liposomes combined with UTMD may reverse early DN. [ 150 , 151 ] bFGF-loaded liposome 170 STZ-induced diabetic male Sprague-Dawley rats bFGF-loaded liposomes combined with UTMD may reverse early DN by inhibiting inflammation. [ 152 ] CREKA-coupled celastrol-loaded liposomes 110 UUO model in C57BL/6 J male mice CREKA-coupled liposomes enable anti-fibrotic drug (celastrol) targeting via fibronectin in fibrotic kidneys.…”

Assessment of the Preventive Effect Against Diabetic Cardiomyopathy of FGF1-Loaded Nanoliposomes Combined With Microbubble Cavitation by Ultrasound

“…Under the energy of diagnostic or therapeutic ultrasound, ultrasound microbubbles can explode in the region of interest or target tissues. Cavitation and mechanical effects of blasting can increase the permeability of local vascular walls or cell membranes, thereby increasing the dose of drugs/genes in target organs or target tissues and their corresponding biological effects (Frenkel, 2008;Chen et al, 2018;Liang et al, 2018;Lin et al, 2018;Yang et al, 2019).…”