Cord and Maternal Blood Monocyte-Macrophage Mediated Antibody-Dependent Cytotoxicity to Herpes Simplex Infected Cells

Abstract

Rabbit antisera to autologous T and R lvmphohlast cell lines HSB-2 and SR, after reciprocal absorption, were found to detect human T and R lymphocyte antigens (HTLA .'nd HBLA antigens). When tested by indirect imnunofluorescence and C' dependent cytotoxicity, the anti-T cell line serum reacted with T cells but not B cells whereas the anti-B cell line serum reacted with B cells but not T cells. These two antisera also reacted with peripheral blood null cells prepared hy nylon column filtration and E rosette depletion. The sum of the percentages of null cells reacting with each antiserum approximated 100%. Null cell suspensions depleted of cells reacting with one antiserum showed a reciprocal increase in cells reacting with the opposite antiserum.These The suppression of CMI during some acute viral infections, including CMV infections, has been postulated to account for the persistence of these viruses in humans and experimental animals. We have developed an experimental animal model in C3H mice to study CHI during this viral infection. The response of splenic lymphocytes from murine CMV(MCMV) infected mice to phytohemagglutinin(PHA) and lipopolysaccharide(LPS) was suppressed during the acute phase of the infection with maximal suppression on day 4 (25% of control for PHA and 28% of control for LPS) and returned to normal by day 15 The mixed lymphocyte reaction(MLR) of splenic lymphocytes from infected animals was suppressed to 38% of control on day 2, to 9% on day 4, and returned to normal by day 15In contrast, lymphocytes responded to MCMV-infected ayngeneic mo se fibroblasts(MEF) by day 2 incorporating 52.183? Y 9866 CPM of H-thymidine compared to 23.58723909 CPM with uninfected MEF. The response to infected MEF increased to 132,560t 22.739 CPM by day LO These data show that lymphocytes responsive to MCMV antigen are generated at the same time that suppression of the response to mitogens and the MLR is observed. These data suggest that the use of non-specific lymphocyte reactions as an index of CMI may provide misleading information and that the suppressions of such reactions may be a result of the regulation of the specific immune response of the host to the viral infection rather than to direct imnunosuppressive effects of the virus itself. NEONATAL ANDROGEN LEVELS AND ANTIBODY PRODUCTION.705 Jean F. Kenny and Pamela C. Pangburn. Univ. of Pittsburgh Sch. of Med., Dept. of Ped., Pittsburgh. Pa. While data suggest that the effects of sex hormones mainly estrogen(E). may be the basis for sex differences in immunity in later 1ife.the reason for the differences in infancy are obscure. Though serum levels of E in male and female babies arelowand not significant,recent data show marked sex differences in serum concentrations(C)of testosterone(T)with levels in males of 0-3months ranging from 350pg/ml to 2000pg/ml and those in females from 50-100pg/ml. To determine what effects these C of T might have on numbers of anti-bacterial antibody producing cells (APC)spleen cells from outbred mcle Swiss mice injected with 3xl...

Preparation of Stable Target Cells for Anti‐Herpes Simplex Virus Cytotoxic T Lymphocytes

“…This failure of humoral immunity is effectively supplemented by the cell-mediated effector system against HSV infection. The antibody dependent cell-mediated cytotoxicity (ADCC) is one of the effectors against HSV-infected cells for which the in vitro measurement using anti-HSV IgG antibody and mononuclear leukocytes (K cell) demonstrated the HSV-specific cytotoxic reaction (7,9,34). The role of ADCC, however, seems to be auxiliary and is not a leading mechanism to protect against HSV infection since the K cells are known to com-prise a minute population of the circulating leukocytes (37), and since the effector function is available only in the late phase of the infection when anti-HSV antibody production is achieved in the affected hosts.…”

Antibody-dependent cellular cytotoxicity to human colon-tumour cells. I. Lack of tumour specificity in a population study

“…It, has to be emphasized that the Ficolle-Hypaqueseparated mononuclear cell preparations may contain substantial numbers of macrophages (Zucker-Franklin, 1974) and chloroacetate-esterase-positive cells and that the percentage of the last-mentionied cells may be especially high in cancer patients (Currie et al, 1978). Although such cells may participate in ADCC (Kohl et al, 1977;GXale & Zighelboim, 1975) (Shoham & Cohen, 1979), which brings evidence that the reaction observed is to an antigenic determinant common to CEA and A, and maybe normal colon antigen too. This determinant most probably resides in the protein portion of the molecule.…”

“…The "null" cell population was not further analysed for the presence of non-lymphocyte white blood cells (ZuckerFranklin, 1974;Currie et al, 1978). Nevertheless, we will refer conventionally to these mononuclear cell preparations as peripheralblood lymphocytes (PBL) with the understanding that other white blood cells present in these preparations may contribute to the ADCC reaction (Kohl et al, 1977;Gale & Zighelboim, 1975 (Tables I and II). This is the case with most of the PBL samples tested, but not with all (Fig.…”

“…The specificity of the reaction is determined by the antibodies that coat the target cells. The nonspecific cellular effector component is the K lymphocyte (Perlman, 1976) or sometimes the macrophage (Kohl et al, 1977) or polymorphonuclear leucocyte (Gale & Zighelboim, 1975). All of them recognize the Fc portion of the coating antibody.…”

Human Fetal Antibody-Dependent Cellular Cytotoxicity to Herpes Simplex Virus–Infected Cells

“…As previously described in detail, ADCC was determined with HE strain HSV I-infected "Cr-labeled Chang liver target cells approximately 24 h after infection (6)(7)(8). Effector cells were added to target cells to a final volume of 0.2 mL in 96-well round-bottom microtiter plates (Coming Glass Works.…”