Cord blood red cell osmotic fragility: a comparison between preterm and full-term newborn infants

Bautista, Maria Lourdes; Altaf, Waseem; Lall, Ritu; Wapnir, Raul A.

doi:10.1016/s0378-3782(03)00009-4

Cited by 22 publications

(16 citation statements)

References 14 publications

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“…Some authors reported that osmotic fragility is decreased in newborn infants as compared with adults [4], while others showed similar osmotic hemolysis with a wider range [23][24][25][26]. Since macrocytosis can affect the results of osmotic fragility tests [24][25][26][27], controls must be age-matched. (2) Family history can be absent (recessive inheritance pattern or de novo mutations) or merely omitted by the parents, especially if a splenectomy has been performed long ago or if HS expression is mild.…”

Section: Discussionmentioning

confidence: 99%

Incidence of Hereditary Spherocytosis in a Population of Jaundiced Neonates

Saada

Cynober

Brossard

et al. 2006

Pediatric Hematology and Oncology

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As most of hereditary spherocytosis-affected individuals experience jaundice at birth, it seemed of interest to evaluate the proportion of hereditary spherocytosis in 402 severely jaundiced neonates with a bilirubinemia level prompting phototherapy. Red cell dehydration, a hallmark of spherocytosis whether constitutional or acquired, was demonstrated in 74 of them, among whom 23 disclosed a typical pattern of spherocytosis upon red cell deformability studies. Acquired spherocytosis of immune origin was diagnosed in 19/23 and hereditary spherocytosis in 4, making the proportion of hereditary spherocytosis-affected individuals among a severely jaundiced population of neonates amount to 1%, an incidence at least 30-fold that of the overall population.

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Section: Discussionmentioning

confidence: 99%

Incidence of Hereditary Spherocytosis in a Population of Jaundiced Neonates

Saada

Cynober

Brossard

et al. 2006

Pediatric Hematology and Oncology

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“…Glycerol is highly lipophilic and can be used in vitro to lyse cells (30) and, when used as a cryoprotectant, glycerol is known to disrupt the integrity of the membrane and the actin cytoskeleton of spermatozoa (31–32). Since our study indicates that glycerol readily penetrates brain tissues, then perhaps glycerol produces cerebral hemorrhage by directly lysing susceptible endothelial membranes including those in the germinal matrix.…”

Section: Discussionmentioning

confidence: 99%

Systemic glycerol decreases neonatal rabbit brain and cerebellar growth independent of intraventricular hemorrhage

et al. 2013

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Background Cerebellar hypoplasia is common problem for preterm infants, and infants that suffer intraventricular hemorrhage (IVH). To evaluate the effects of IVH on cerebellar growth and development, we used a neonatal rabbit model of systemic glycerol to produce IVH. Methods New Zealand White rabbit kits were surgically delivered 2 d preterm, and treated with i.p. glycerol (3.25 to 6.5 g/kg). Controls were born at term. IVH was documented by ultrasound. Brain MRI volumes, cerebellar foliation, proliferation (Ki-67) and Purkinje cell density were done at two weeks of life. Tissue glycerol and glutathione concentrations were measured. Results Glycerol increased IVH, subarachnoid hemorrhages and mortality in a dose-dependent manner. Total cerebellar volumes, cerebellar foliation and cerebellar proliferation were decreased in a dose-dependent manner. Glycerol accumulated rapidly in blood, brain and liver and was associated with increased glutathione concentration. All of these results were independent of IVH status. Conclusions Cerebellar hypoplasia was induced after glycerol administration in a dose-dependent manner. Given rapid tissue accumulation of glycerol, dose dependent decreased brain growth and lack of IVH effect on measured outcomes we question the validity of this model as glycerol toxicity cannot be ruled out. A more physiologic model of IVH is needed.

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“…Previous work using light scattering or hemolysis techniques have reported that cord RBCs are more deformable than adult RBCs, 9,10 whereas electron spin resonance and filtration studies have concluded that there are no significant differences in cell deformability between cord RBCs and adult RBCs. In particular, the morphological and mechanical properties of cord RBCs in newborn infants, which are closely related to the deformability of cord RBCs and cord-blood circulation, have not been fully addressed largely because of the limitations in measurement techniques.…”

Section: Introductionmentioning

confidence: 95%

Three-dimensional refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood

Park

Ahn

et al. 2015

J. Biomed. Opt

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Red blood cells (RBCs) from the cord blood of newborn infants have distinctive functions in fetal and infant development. To systematically investigate the biophysical characteristics of individual cord RBCs in newborn infants, a comparative study was performed on RBCs from the cord blood of newborn infants and from adult mothers or nonpregnant women using optical holographic microtomography. Optical measurements of the distributions of the three-dimensional refractive indices and the dynamic membrane fluctuations of individual RBCs were used to investigate the morphological, biochemical, and mechanical properties of cord, maternal, and adult RBCs at the individual cell level. The volume and surface area of the cord RBCs were significantly larger than those of the RBCs from nonpregnant women, and the cord RBCs had more flattened shapes than that of the RBCs in adults. In addition, the hemoglobin (Hb) content in the cord RBCs from newborns was significantly higher. The Hb concentration in the cord RBCs was higher than that in the nonpregnant women or maternal RBCs, but they were within the physiological range of adults. Interestingly, the amplitudes of the dynamic membrane fluctuations in cord RBCs were comparable to those in nonpregnant women and maternal RBCs, suggesting that the deformability of cord RBCs is similar to that of healthy RBCs in adults.

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Cord blood red cell osmotic fragility: a comparison between preterm and full-term newborn infants

Cited by 22 publications

References 14 publications

Incidence of Hereditary Spherocytosis in a Population of Jaundiced Neonates

Incidence of Hereditary Spherocytosis in a Population of Jaundiced Neonates

Systemic glycerol decreases neonatal rabbit brain and cerebellar growth independent of intraventricular hemorrhage

Three-dimensional refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood

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