Cordycepin decreases activity of hippocampal CA1 pyramidal neuron through membrane hyperpolarization

Cordycepin Suppresses Excitatory Synaptic Transmission in Rat Hippocampal Slices Via a Presynaptic Mechanism

“…Interestingly, our previous study showed that cordycepin could reduce neuronal activity by inducing membrane hyperpolarization . In combination with our previous study , the results in this study poses a strong insight that the suppression effect of cordycepin on the excitatory synaptic transmission is an important mechanism involved in the affections of neuron membrane hyperpolarization. This pathway may in turn contribute to the protective effects of cordycepin on CA1 neurons from glutamate excitotoxicity damage in the pathogenesis of postischemic cell death and other excitotoxic disorders.…”

“…No matter to which, one important common pathophysiological mechanism of excitotoxic neuronal death may result from an extended neuronal depolarization induced by excess/excitotoxic glutamate exposure . Interestingly, our previous study showed that cordycepin could reduce neuronal activity by inducing membrane hyperpolarization . In combination with our previous study , the results in this study poses a strong insight that the suppression effect of cordycepin on the excitatory synaptic transmission is an important mechanism involved in the affections of neuron membrane hyperpolarization.…”

“…Our previous study has shown that cordycepin can decrease the neuron activity through membrane hyperpolarization , which indicated a novel potential mechanism of cordycepin in modulating the responses of CNS by adjusting its electrophysiologic properties. To better understand the modulating function of cordycepin on the CNS, we examined the effects of cordycepin on excitatory synaptic transmission in the CA1 region of rat hippocampal brain slices by using in vitro field potential electrophysiology and whole‐cell patch clamp techniques.…”

Orexin‐A increases the firing activity of hippocampal CA1 neurons through orexin‐1 receptors

“…Both intrinsic membrane properties and extrinsic factors determine the spontaneous firing activity and synaptic transmission of hippocampal neurons (Fricker et al, 1999;Gu et al, 2000;Tian et al, 2010;Yao et al, 2011;Yamada-Hanff and Bean, 2013;Kowalski et al, 2016). Previous studies have indicated that endogenous neurotransmitters or neuromodulators modulate the firing activity of hippocampal CA1 pyramidal neurons.…”

Tenuigenin enhances hippocampal Schaffer collateral-CA1 synaptic transmission through modulating intracellular calcium

“…The method of brain slices preparation was slightly modified from those described previously Yao et al 2011). Briefly, acute hippocampal slices were prepared and maintained in an interface recording chamber containing preheated aCSF of the following composition (in mM): 117 NaCl, 4.7 KCl, 2.5 CaCl 2 , 1.2 MgCl 2 , 25 NaHCO 3 , 1.2 NaH 2 PO 4 , and 11 glucose and maintained at 31 ± 0.5°C, pH 7.35-7.45.…”