Cordyceptin induces apoptosis through repressing hTERT expression and inducing extranuclear export of hTERT

Jang, Kyung‐In; Kwon, Gi-Sun; Jeong, Jin‐Woo; Kim, Cheol-Hong; Yoon, Hyun‐Min; Kim, Gi‐Young; Shim, Jung‐Hyun; Moon, Sung‐Kwon; Kim, Wun-Jae; Choi, Yung Hyun

doi:10.1016/j.jbiosc.2014.08.008

Cited by 16 publications

(15 citation statements)

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“…Therefore, we analyzed whether this signaling pathway was involved in the induction of T24 cell apoptosis by cordycepin, and found cordycepin suppressed the phosphorylated levels of Akt, as well as PI3K. This means that the PI3K/Akt signaling pathway is inactivated by cordycepin treatment, and the results are in good agreement with previous studies performed on several other cancer cell lines (31)(32)(33)(34)(35)(36)42,51). Furthermore, in line with our previous study using leukemia cells (36), LY294002, a pharmacological inhibitor of PI3K, significantly enhanced the apoptotic effect of cordycepin and further reduced cell viability, supposing that cordycepin-induced apoptosis is mediated by blocking the PI3K/Akt signaling pathway.…”

Section: Discussionsupporting

confidence: 89%

“…It has also been reported that several cellular signaling pathways are involved in the induction of apoptosis of cancer cells by cordycepin (27,32,35,(38)(39)(40), and that the accumulation of ROS associated with mitochondrial dysfunction acts as a major signal in this process (27,(29)(30)32,39,41). Although the possibility of involvement of PI3K/Akt signaling pathway was suggested in several previous studies (32)(33)(34)(35)(36)42), the link between this pathway and the production of ROS is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

“…It has also been found that the increase of ROS production in leukemia, gastric and prostate cancer cells plays an important role in the induction of intrinsic apoptosis pathway (28)(29)(30). In addition, the anticancer effects of cordycepin involve the disturbance of various cell signaling pathways, and in particular, cordycepininduced apoptosis in human gastric and ovarian cancer cells and leukemia and glioma cells was accompanied by inactivation of the PI3K/Akt signaling pathway (31)(32)(33)(34)(35)(36). Although the induction of apoptosis by cordycepin in a certain gastric cancer cell line was accompanied by the production of ROS and inactivation of the PI3K/Akt signaling pathway (24), the underlying mechanism of ROS involved in the inactivation of PI3K/Akt signaling pathway by cordycepin is still not well known.…”

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confidence: 99%

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Cordycepin induces apoptosis in human bladder cancer T24 cells through ROS-dependent inhibition of the PI3K/Akt signaling pathway

Kim

Jae

Park

et al. 2019

BST

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Cordycepin induces apoptosis in human bladder cancer T24 cells through ROS-dependent inhibition of the PI3K/Akt signaling pathway

Kim

Jae

Park

et al. 2019

BST

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“…Previous evidence also suggests that ROS play a critical role as signaling molecules that regulate the PI3K/Akt signaling pathway [Hsieh and Yang, 2013;Zhang et al, 2016]. The activation of PI3K/Akt consequently plays an important role in triggering telomerase activation through the nuclear translocation of hTERT and in controlling cell fate [Jayasooriya et al, 2012;Jang et al, 2015;Li et al, 2016]. Our data also showed that telomerase activity was decreased by the inhibition of PI3K/Akt signaling in fucoidan-treated 5637 cells (Fig.…”

Section: Discussionsupporting

confidence: 79%

Fucoidan Induces ROS‐Dependent Apoptosis in 5637 Human Bladder Cancer Cells by Downregulating Telomerase Activity via Inactivation of the PI3K/Akt Signaling Pathway

Han

Lee

Jeong

et al. 2016

Drug Development Research

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Preclinical Research Fucoidan, a sulfated polysaccharide, is a compound found in various species of seaweed that has anti-viral, anti-bacterial, anti-oxidant, anti-inflammatory, and immunomodulatory activities; however, the underlying relationship between apoptosis and anti-telomerase activity has not been investigated. Here, we report that fucoidan-induced apoptosis in 5637 human bladder cancer cells was associated with an increase in the Bax/Bcl-2 ratio, the dissipation of the mitochondrial membrane potential (MMP, Δψm), and cytosolic release of cytochrome c from the mitochondria. Under the same experimental conditions, fucoidan-treatment decreased hTERT (human telomerase reverse transcriptase) expression and the transcription factors, c-myc and Sp1. This was accompanied by decreased telomerase activity. Fucoidan-treatment also suppressed activation of the PI3K/Akt signaling pathway. Inhibition of PI3K/Akt signaling enhanced fucoidan-induced apoptosis and anti-telomerase activity. Meanwhile, fucoidan treatment increased the generation of intracellular ROS, whereas the over-elimination of ROS by N-acetylcysteine, an anti-oxidant, attenuated fucoidan-induced apoptosis, inhibition of hTERT, c-myc, and Sp1 expression, and reversed fucoidan-induced inactivation of the PI3K/Akt signaling pathway. Collectively, these data indicate that the induction of apoptosis and the inhibition of telomerase activity by fucoidan are mediated via ROS-dependent inactivation of the PI3K/Akt pathway. Drug Dev Res 78 : 37-48, 2017. © 2016 Wiley Periodicals, Inc.

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“…In general, those drugs often anti-metabolites that interfere with nucleic acid or protein synthesis can suppress the immune response. The greatest problem facing the physician is to effectively balance at the same time the dosages of drugs or irradiation so that the immune system is suppressed against the graft, but is still capable of defense against environmental pathogens [32][33][34][35][36][37][38][39][40][41][42].…”

Section: Acquired Immuno-suppressionmentioning

confidence: 99%

A Constitution Dependent QOL Assessment

Yamaguchi¹

2017

J Tradi Med Clin Natur

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According to the reports from Ministry of Health in each country, the average life spans were expanded over the past century. This trend is expected to be extended further in the future and at the same time continuous efforts should be made. That indicates that the quantitative objective, as one of the objectives people have aimed at since ancient times to attain the perpetual youth and longevity, are almost achieved in this way. However, as the next objective, it is necessary for us to be concerned about improving the more advanced quality of life.In an effort of improving the quality of life, other than the western medicine, we have attempted to bring many traditional medical practices, including the Oriental medicine, from various parts of the world into the medical field as alternative medicine. Judging both positive and negative aspects by the evaluation standard of the Western medicine, we try to obtain numerical values of QOL, and after that, set a numerical target for improvement of QOL. Some methods of alternative medicine taken in this review are acupuncture moxibustion, Chinese medicine and other traditional medicine. Above all, WHO suggests selecting the suzerain nation of acupuncture moxibustion, which has been developed in the Oriental countries and realized as a specialized cure, by raising good & bad points and ascertaining which nation advocating the best modeling method could be. At the moment for evaluating CAM, for example, what kind of methods is/are suitable for evaluating each CAM. We have been trying to propose the peripheral leukocyte is one of the best ways for evaluating CAM. Our trials are hot spring hydro therapy, acupuncture and moxibution, light exercise and undle heating. In these evaluations, we found the common results from leukocyte effects which exhibited strong correlation for the regulation. The contents are the result from the data showing the much in number tend to downly regulate, on the other hand lower number one is up-regulated. This kind of regulation showed within a 24 hrs, for the leukocyte subsets, granulocyte and lymphocyte are changed under circadian rhythm. So for purpose of evaluating leukocyte deviation, we set the point for evaluation at the same-time zone from the first set of evaluation. Under these condition, we got a same results reproductively that the whole number of leukocyte, and its subset, granulocyte and lymphocyte also regulated within a 24 hrs. This kind of phenomenon is the case that we confirm a lot of kind of CAM and repetition. However, the life span of leukocyte are at lease 3-4 days and no such a drastic apoptosis was induced such a stimulation. For the purpose of scientific explanation, we proposed that the emotional hormone are concern this change of leukocytes population. For the results of this hypothesis, there were reasonable change were seen in the peripheral blood about emotional hormone, adrenaline and dopamine. Other hormone concern thyroid grand was not changed significantly. In this review, I plan to collect evidence and...

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Cordyceptin induces apoptosis through repressing hTERT expression and inducing extranuclear export of hTERT

Cited by 16 publications

References 48 publications

Cordycepin induces apoptosis in human bladder cancer T24 cells through ROS-dependent inhibition of the PI3K/Akt signaling pathway

Cordycepin induces apoptosis in human bladder cancer T24 cells through ROS-dependent inhibition of the PI3K/Akt signaling pathway

Fucoidan Induces ROS‐Dependent Apoptosis in 5637 Human Bladder Cancer Cells by Downregulating Telomerase Activity via Inactivation of the PI3K/Akt Signaling Pathway

A Constitution Dependent QOL Assessment

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