Coriander Alleviates 2,4-Dinitrochlorobenzene-Induced Contact Dermatitis-Like Skin Lesions in Mice

Abstract

Contact dermatitis (CD) is a pattern of inflammatory responses in the skin that occurs through contact with external factors. The clinical picture is a polymorphic pattern of skin inflammation characterized by a wide range of clinical features, including itching, redness, scaling, and erythema. Coriandrum sativum L. (CS), commonly known as coriander, is a member of the Apiaceae family and is cultivated throughout the world for its nutritional and culinary values. Linoleic acid and linolenic acid in CS have various pharmacological activities. However, no study of the inhibitory effects of CS on CD has been reported. In this study, we demonstrated the protective effect of CS against 2,4-dinitrochlorobenzene-induced CD-like skin lesions. CS, at doses of 0.5-1%, applied to the dorsal skin inhibited the development of CD-like skin lesions. Moreover, the Th2-mediated inflammatory cytokines, immunoglobulin E, tumor necrosis factor-α, interferon-γ, interleukin (IL)-1, IL-4, and IL-13, were significantly reduced. In addition, CS increased the levels of total glutathione and heme oxygenase-1 protein. Thus, CS can inhibit the development of CD-like skin lesions in mice by regulating immune mediators and may be an effective alternative therapy for contact diseases.

Melatonin inhibits neuronal dysfunction‐associated with neuroinflammation by atopic psychological stress in NC/Nga atopic‐like mouse models

“…Scratching behavior and clinical skin severity scores were assessed, and hematoxylin and eosin staining was performed, according to previously published methods . Briefly, mice were placed individually in clear plastic cages and their behavior was monitored for 30 minute, 7 weeks after sensitization.…”

“…The estimated product size of each reaction product of MT1R was 248 bp. In addition, western blotting was performed according to previously published methods . The tissues were lysed by homogenization with protease inhibitor cocktail (Roche, Mannheim, Germany) with added triple‐detergent lysis buffer (50 mmol/L Tris‐HCl, 150 mmol/L NaCl, 0.1% sodium dodecyl sulfate (SDS), 1% NP‐40) to detect MT1.…”

“…Scratching behavior and clinical skin severity scores were assessed, and hematoxylin and eosin staining was performed, according to previously published methods. 40,41 Briefly, mice were placed individually in clear plastic cages and their behavior was monitored for 30 minute, 7 weeks after sensitization. Scratching of the rostral back and biting of the caudal back were observed; scratching movements by the hind paw were defined as a scratching bout that ended when the mice either licked its hind paw or placed its hind paw back on the floor, and a series of one or more biting movements were counted as one episode that ended when the rat returned to the straightforward position.…”

“…In addition, western blotting was performed according to previously published methods. [40][41][42] The tissues were lysed by homogenization with protease inhibitor cocktail (Roche, Mannheim, Germany) with added triple-detergent lysis buffer (50 mmol/L Tris-HCl, 150 mmol/L NaCl, 0.1% sodium dodecyl sulfate (SDS), 1% NP-40) to detect MT1. The fractionation lysates were separated by 10%-15% SDS-PAGE, and were then transferred to a membrane.…”

A review on chemical constituents and pharmacological activities of Coriandrum sativum

“…In addition, CS increased the levels of total glutathione and heme oxygenase-1 protein. Thus, CS inhibited the development of CD-like skin lesions in mice by regulating immune mediators and may be an effective alternative therapy for contact diseases [170].…”