Corn-derived carbohydrate inositol hexaphosphate inhibits Barrett's adenocarcinoma growth by pro-apoptotic mechanisms

Abstract

Abstract. Inositol hexaphosphate (IP6) is a naturally occurring polyphosphorylated carbohydrate that is found in food sources high in fiber content. IP6 has been reported to have significant inhibitory effects against a variety of primary tumors. We hypothesized that IP6 would inhibit the cell growth rate of Barrett's adenocarcinoma in vitro. Two Barrett'sassociated adenocarcinoma cell lines, SEG-1 and BIC-1, were treated with IP6 at 0.5, 1.0 and 5.0 mM concentrations. Cell viability was measured by MTT assay. Apoptosis and necrosis were evaluated by the Annexin V FITC assay. Reductions (P<0.001) in cellular proliferation were observed in both cell lines. IP6 decreased late apoptosis and necrosis in BIC cells, whereas in SEG-1 cells, early apoptosis, late apoptosis and necrosis were all increased by IP6. IP6 decreases cellular growth by pro-apoptotic mechanisms. Our findings suggest that IP6 has the potential to become an effective adjunct for Barrett's adenocarcinoma. Further studies are needed to evaluate safety and clinical utility of this agent in patients with Barrett's adenocarcinoma.

Glycemic index, carbohydrate and fiber intakes and risk of reflux esophagitis, Barrett’s esophagus, and esophageal adenocarcinoma

“…A recent study demonstrated that phytic acid, which is found in highfiber foods, inhibited the cell growth rate of esophageal adenocarcinoma in vitro by reducing cellular proliferation and promoting apoptosis [32]. High-fiber diets have been associated with lower plasma concentrations of markers of systemic inflammation, such as interleukin-6, which may play an important role in carcinogenesis [33].…”

Dietary fiber and the risk of precancerous lesions and cancer of the esophagus: a systematic review and meta-analysis

“…An inverse association between fiber intake and esophageal cancer is biologically plausible. Phytic acid, which is present in high‐fiber foods, has been demonstrated to decrease cellular proliferation and promote apoptosis, resulting in the inhibition of esophageal adenocarcinoma cell growth in vitro, but has yet to be explored in the etiology of squamous cell carcinoma. Reduced concentrations of interleukin 6, a marker of systemic inflammation, have also been associated with high‐fiber diets and may be involved in tumor development .…”

Dietary factors and the risks of oesophageal adenocarcinoma and Barrett's oesophagus

“…A recent study demonstrated that inositol hexaphosphate, a naturally occurring polyphosphorylated carbohydrate found in food sources high in fiber, inhibited the cell growth rate of Barrett’s-associated esophageal adenocarcinoma cells in vitro by reducing cellular proliferation and promoting apoptosis. (79) In addition, a diet rich in fiber is associated with lower plasma levels of biomarkers of systemic inflammation such as tumor necrosis factor-alpha (TNF-α) receptor-2 and interleukin-6, potentially affecting the carcinogenesis process. (80) Also, fiber itself may absorb carcinogens from food items that pass through the digestive tract,(35) or reduce the risk of Barrett’s esophagus by decreasing the risk of hiatal hernia.…”