An investigation was conducted to determine if N compounds and other chemical parameters that could cause poor corn (Zea mtJys L.) growth were associated with injected liquid beef manure. Liquid beef manure was injected into a Derinda silt loam soil (tine, mixed, mesic Typic Hapludalf) and incubated in two laboratory experiments. Experiment 1 consisted of two application rates, 18 and 36 mL kg-' soil, injected into 25 g of moist soil and incubated for 44 d. Experiment 2 consisted of five manure application rates (0, 29, 58, 116, and 232 mL kg-' soil, equivalent to 2.3, 4.6, 9.2, and 18.4 dry Mg ba-') layered between SO g of soil and incubated for 4 wk. Rates simulated the range of manure concentrations in band applications of 37200 L manure ha · 1 • There was a linear trend of increasing NH.-N concentrations as manure application rates increased. A transitory period of lowered soil redox potential (E 0 ) and elevated pH occurred for 2 wk after application, especially for the highest manure rate. Nitrite-N accumulated with all rates of manure, but the accumulation was transitory with low manure rates. At 116 and 232 mL kg-', higb levels of N0 2 -N were present for 3 and 4 wk, respectively. Conditions potentially toxic to plants were generated initially by reducing conditions (as indicated by low E.l and free NH 3 (due to high pH and high concentrations of water extractable and exchangeable NH 4 -N), and subsequently, by accumulation of N0 2 -N.