Corneal epithelial wound healing.

Dua, Harminder S.; Gomes, José Álvaro Pereira; Singh, Ajit

doi:10.1136/bjo.78.5.401

Cited by 267 publications

(164 citation statements)

References 39 publications

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“…A similar 'streaming' of fluorescein-stained cells in columns has also been observed in relation to broken sutures following corneal grafting. 31 These observations lend support to the belief that SC activity does not occur contiguously along the limbus but rather in an interrupted manner presumably corresponding to repositories of SC in the rete ridges that alternate with palisades, which may not hold a similar mass of SC.…”

Section: Clinical Evidence For Limbal Location Of Putative Corneal Epsupporting

confidence: 61%

“…The clinical features of SC deficiency, from mild to severe, include the following: 19,30,31,[61][62][63][64][65] (a) loss of limbal anatomy; (b) irregular, thin epithelium; (c) stippled fluorescein staining of the area covered by abnormal epithelium; (d) unstable tear film; (e) filaments and erosions; (f) superficial and deep vascularisation; (g) persistent epithelial defects leading to ulceration, melting, and perforation, (h) fibrovascular pannus; and (i) scarring, keratinisation, and calcification.…”

Section: Effects Of Deficiencymentioning

confidence: 99%

“…The abnormal fluorescein-staining 'conjunctivalised' epithelium may take on the pattern of Stem cell differentiation HS Dua et al 882 columns, whorls, or wedges with the broad base towards the limbus and the narrow curving apex towards the corneal centre (Figure 6b). 31 Unstable tear film: The abnormal epithelium demonstrates a rapid tear film break-up time over it, and areas of negative and positive fluorescein staining can be seen.…”

Section: Effects Of Deficiencymentioning

confidence: 99%

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Stem cell differentiation and the effects of deficiency

Dua

Joseph

Shanmuganathan

et al. 2003

Eye

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195

141

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Stem cells have several unique attributes, the key features being their potency and plasticity. They have the ability to give rise to multiple cell lineages and to transdifferentiate into totally different cell type(s) when relocated to a novel stem cell niche. Most self-renewing tissues are served by stem cells. At the ocular surface, the corneo-scleral limbus is believed to provide the niche for corneal epithelial stem cells. A large body of circumstantial evidence, both clinical and basic, supports this view. However, specific identification of limbal stem cells has proved elusive. Cytokeratin markers, vimentin, epidermal growth factor receptors, p63, and others have been used to identify epithelial cell populations at the limbus, which could harbour putative stem cells. In contrast, none of the known haematopoietic stem cell markers namely, CD34 and CD133, stain any specific subset of corneal or limbal epithelial cells. Singly or collectively, none of these markers point to any unique cell(s) that could be regarded as stem cells, supporting the notion that the corneal epithelium is served by 'committed progenitors' rather than by stem cells. Disease or destruction of the corneoscleral limbus is associated with consequential events that eventually lead to visual impairment or blindness. Conjunctivalisation and vascularisation of the corneal surface and persistent or recurring epithelial defects are hallmarks of limbal deficiency.

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Section: Clinical Evidence For Limbal Location Of Putative Corneal Epsupporting

confidence: 61%

Section: Effects Of Deficiencymentioning

confidence: 99%

Section: Effects Of Deficiencymentioning

confidence: 99%

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Stem cell differentiation and the effects of deficiency

Dua

Joseph

Shanmuganathan

et al. 2003

Eye

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“…A mais importante é a presença de vasos no limbo nutrindo o estroma corneano periférico (39) . As paliçadas de Vogt (elevações fibrovasculares radiais) permitem o aporte sangüíneo bem próximo ao estroma corneano periférico (40) .…”

Section: Grupo IIunclassified

Avaliação espectrofotométrica do azul de Evans na reação inflamatória da córnea: estudo experimental em coelhos

Gehlen¹,

Moreira²,

Moreira³

et al. 2004

Arq. Bras. Oftalmol.

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Avaliação espectrofotométrica do azul de Evans na reação inflamatória da córnea: estudo experimental em coelhosObjetivo: Constatar a presença do azul de Evans na córnea normal estipulando o período de tempo de concentração máxima do corante após inoculação endovenosa e estudar a permeabilidade vascular em modelo animal da inflamação corneal induzida por queimadura química por meio de injeção de azul de Evans. Métodos: Cinquenta coelhos foram divididos em 3 grupos: Grupo I (25 animais): injetou-se 20 mg/kg de azul de Evans e os animais foram sacrificados após 8, 10, 12, 14 e 16 horas. Retirou-se a córnea e quantificou-se o corante por meio de micrométodo espectrofotométrico. Grupo II: em 15 animais injetou-se o corante e, após 10 horas, fragmentouse centralmente o tecido com trépanos de 6, 8 e 10 mm. Procedeu-se à extração do azul de Evans da mesma forma que no grupo I. Grupo III: induziu-se queimadura na córnea do olho direito de 10 animais com NaOH a 1 N. Cinco dias após o procedimento, os animais foram sacrificados, sendo que, 10 horas antes do sacrifício, foi inoculado o azul de Evans para que posteriormente se pudesse quantificá-lo. A córnea esquerda serviu como controle. Resultados: No grupo I, a média da concentração do azul de Evans às 10 h. foi de 15,28 ± 0,09 µg/mg. No grupo II, as médias das concentrações do corante foram: 6 mm: 0,93 ± 0,01µg/mg; 8 mm: 1,20 ± 0,06 µg/mg; 10 mm: 1,32 ± 0,05 µg/mg. No grupo III, as médias das concentrações do azul de Evans foram: olho direito (queimadura): 23,74 ± 2,64 µg/mg e olho esquerdo (controle): 16,71 ± 2,04 µg/mg. Conclusões: Quantificou-se o azul de Evans pela primeira vez na córnea de coelhos e constatou-se que, após 10 horas de inoculação endovenosa, o corante atingiu seu pico de concentração no tecido. Concluiu-se que o azul de Evans serve como bom método de quantificação da permeabilidade vascular alterada na córnea de coelhos. INTRODUÇÃODesde 1943, com os primeiros estudos de Rawson, o azul de Evans tem sido amplamente utilizado em diversos trabalhos experimentais. Este corante apresenta algumas características como inocuidade (1)(2) , alta solubilidade em água e afinidade pela albumina (3)(4)(5) . O azul de Evans, quando inoculado via endovenosa, combina-se com a albumina de maneira reversível e dependente de pH (4) .Estudos demonstraram que, após 40 horas da administração endovenosa do azul de Evans em ratos, 90% do corante já havia deixado a circulação, atingindo diversos tecidos do organismo. Depois de 24 horas de inoculação

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“…In such occasions, there is growth of the conjunctival epithelium onto the corneal surface (conjunctivalization), as well as chronic inflammation, superficial vascularization, calcification, ulceration, melting and perforation of the cornea (Swift et al, 1996;Tseng and Tsubota, 1997;Dua and AzuaraBlanco, 1999;Dua and Azuara-Blanco, 2000). When corneal surface is covered by conjunctival epithelium, goblet cells can be seen in this specialized part of the fibrous tunic (Dua, 1998;Schwab, 1999).…”

Section: Introductionmentioning

confidence: 99%

Sclerocorneal limbal stem cell autograft transplantation in dogs

Brunelli

Vicente

Chahud

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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The effects of sclerocorneal limbal stem cell autograft transplantation in dogs with corneal wounds were studied. Eighteen dogs were divided in two groups (GI and GII). The animals of GI (n=12) underwent limbal transplantation 30 days after the destruction of limbal stem cells. The dogs of GII (n=6) only underwent destruction of stem cells (control group). Light microscopy examination of the right eye was performed on days 3, 7, 14, 30, 60, and 120 after limbal transplantation (GI), and on days 33, 37, 44, 60, 90, and 150 after limbal destruction (GII). Results showed a complete destruction of limbal stem cells with loss of corneal transparency. Limbal transplantation prevented conjunctivalization in grafted area. Corneal vascularization and a 360º corneal conjunctivalization were noted in the control dogs (GII). Corneal transparency was restored from day 60th after surgery. Histological examination did not distinguish the transition between the graft and the normal corneal epithelium at anytime. Goblet cells were found in control animals (GII) on 33, 37, 60, and 150 days, whereas a single grafted dog (GI) presented a few goblet cells on day 60th post-transplantation. Limbal autograft transplantation was effective in restoring corneal clarity with no development of ocular complications.

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Corneal epithelial wound healing.

Cited by 267 publications

References 39 publications

Stem cell differentiation and the effects of deficiency

Stem cell differentiation and the effects of deficiency

Avaliação espectrofotométrica do azul de Evans na reação inflamatória da córnea: estudo experimental em coelhos

Sclerocorneal limbal stem cell autograft transplantation in dogs

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