Corneal stromal ablation with femtosecond ultraviolet pulses in rabbits

Danieliene, Egle; Gabryte, Egle; Danielius, R.; Vengris, Mikas; Vaičeliūnaitė, Agnė; Morkunas, Vaidotas; Rukšėnas, Osvaldas

doi:10.1016/j.jcrs.2012.09.022

Cited by 7 publications

(11 citation statements)

References 25 publications

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“…Previous studies have evaluated the parameters, safety, and performance of various UV femtosecond laser systems. [70][71][72] Due to the shorter wavelength, it has been proposed that UV lasers can create a more accurate focus with less energy per laser pulse. Hammer et al 73 present the results of a study of 25 porcine eyes and 15 rabbit corneas with a new 345 nm UV femtosecond laser.…”

Section: Ultraviolet Femtosecond Lasersmentioning

confidence: 99%

Advances in Refractive Surgery

Pillar

Krueger

2016

Asia-Pacific Journal of Ophthalmology

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Section: Ultraviolet Femtosecond Lasersmentioning

confidence: 99%

Advances in Refractive Surgery

Pillar

Krueger

2016

Asia-Pacific Journal of Ophthalmology

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“…To increase the ablation rate of the corneal stroma, we have modified the laser system that was previously described in detail. 17,18 First, the laser was switched to the standard pulse duration mode (280 AE 10 fs pulses instead of 200 AE 10 fs in the short pulse mode), which is more power efficient. This alteration enabled us to increase the average power of the fundamental laser radiation (1030 nm) up to 5 W at a 50 kHz pulse repetition rate, which in turn resulted in an average UV power up to 400 mW.…”

Section: Femtosecond Laser Systemmentioning

confidence: 99%

“…23 Assuming the super-Gaussian intensity distribution, the peak fluence with these laser settings was 135 AE 5 mJ∕cm 2 , which is close to the value we used in our previous experiments on rabbits. 18 We also modified the scanning pattern to maintain low local heating and to achieve a smooth ablation surface at a higher pulse repetition rate. The tissue was removed by ablating circular layers, each of which consisted of five sublayers.…”

Section: Femtosecond Laser Systemmentioning

confidence: 99%

“…17 Our experiments, in which rabbit corneas were treated in vivo with femtosecond UV pulses, did not show any adverse effects that could be induced specifically by high peak intensity, and the healing responses were similar to those after excimer ablation. 18 To be able to compare the results of ablation due to the two lasers, we adjusted the average power of our femtosecond UV source to that of the excimer laser we had at our disposal. As a result, the ablation was relatively slow, at 3.7 s∕diopter (s∕D), which is well below the industry standards currently accepted.…”

Section: Introductionmentioning

confidence: 99%

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High-speed photorefractive keratectomy with femtosecond ultraviolet pulses

Danieliene¹,

Gabryte

Vengris

et al. 2015

J. Biomed. Opt.

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Femtosecond near-infrared lasers are widely used for a number of ophthalmic procedures, with flap cutting in the laser-assisted in situ keratomileusis (LASIK) surgery being the most frequent one. At the same time, lasers of this type, equipped with harmonic generators, have been shown to deliver enough ultraviolet (UV) power for the second stage of the LASIK procedure, the stromal ablation. However, the speed of the ablation reported so far was well below the currently accepted standards. Our purpose was to perform high-speed photorefractive keratectomy (PRK) with femtosecond UV pulses in rabbits and to evaluate its predictability, reproducibility and healing response. The laser source delivered femtosecond 206 nm pulses with a repetition rate of 50 kHz and an average power of 400 mW. Transepithelial PRK was performed using two different ablation protocols, to a total depth of 110 and 150 μm. The surface temperature was monitored during ablation; haze dynamics and histological samples were evaluated to assess outcomes of the PRK procedure. For comparison, analogous excimer ablation was performed. Increase of the ablation speed up to 1.6 s/diopter for a 6 mm optical zone using femtosecond UV pulses did not significantly impact the healing process.

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“…The femtosecond laser is the new scalpel for biologists. Since 2000, they have gradually become familiar with this tool, which opens up great prospects in life sciences [37].…”

Section: Photo-ablationmentioning

confidence: 99%

The Laser Technology: New Trends in Biology and Medicine

Legrès¹,

Chamot²,

Varna³

et al. 2014

JMP

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In fifty years, laser technology has made great progress, and its many applications make it essential in everyday life. However, this technology is still open to numerous developments. Across multiple applications, there is particular focus in the field of medicine, for diagnosis for tailored therapies, and as a research tool in biology. Whereas its use is now well-demonstrated in ophthalmologic and dermatologic treatments, and surgery, one of the most fascinating aspects of laser technology in the field of biology emerged in the late 1990s with the development of devices able to perform fine dissections of biological tissues using a laser beam. The so-called laser-associated microdissection offers a rapid, precise method of isolating and removing targeted cells or groups of cells from complex biological tissues. It represents the missing link between clinical observations and the intrinsic physiological mechanisms of biological tissues. The molecular examination of pathologically altered cells and tissues for DNA, RNA, and protein expression has revolutionized research and diagnosis in pathology, enabling assessment of the role of the cell type in the normal physiological or disease process. Alongside conventional diagnostic and therapeutic approaches, another field of application contribute to the development of targeted treatments at the nanoscale level of laser technology, mainly in the field of cancer, leading to design new and innovative strategies in drug delivery and image-guided surgery. Most of these approaches, but although not exhaustively, will be presented here.

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Corneal stromal ablation with femtosecond ultraviolet pulses in rabbits

Abstract: Ms. Gabryte and Mr. Danielius are paid employees of Light Conversion Ltd. Mr. Danielius is a shareholder of Light Conversion Ltd. No other author has a financial or proprietary interest in any material or method mentioned.

Cited by 7 publications

References 25 publications

Advances in Refractive Surgery

Advances in Refractive Surgery

High-speed photorefractive keratectomy with femtosecond ultraviolet pulses

The Laser Technology: New Trends in Biology and Medicine

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