Corneal Thickness: Postoperative Changes in Refrigerated and Cryopreserved Corneal Grafts

Ar, Irvine; Ja, Capella; He, Kaufman

doi:10.1001/archopht.1969.00990020234017

Cited by 23 publications

(3 citation statements)

References 5 publications

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“…The figure shows that the endothelium of cryopreserved grafts eventually resumed a normal function, but that this happened later than in fresh and short-time cultured grafts. Late thinning of cryopreserved grafts has earlier been reported by Irvine et al (1969), by Ginsberg et al (1973), and by McCarey et al (1976). The specific reason for this phenomenon is presently unknown.…”

Section: Commentsmentioning

confidence: 79%

Post‐operative Thickness and Endothelial Cell Density in Cultivated, Cryopreserved Human Corneal Grafts

Ehlers

Sperling

Olsen

1982

Acta Ophthalmologica

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Seventeen corneas obtained 2-72 h post mortem, from donors aged 17-78 years, were cultivated for 24 h, cryopreserved, thawed, cultivated for another 24 h and grafted. One year post-operatively 12 of the 17 grafts were clear. In 10 of these 12 cases the visual acuity was greater than or equal to 0.33. One primary graft failure occurred, while 4 primarily clear grafts became cloudy due to glaucoma (2), phthisis bulbi (1) and herpetic reinfection (1). One year after the transplantations the central thickness of the clear grafts was 0.51 mm, and the endothelial cell density was 1028 cells/mm2, corresponding to 32% of the cell density before cryopreservation. This endothelial cell loss was not correlated to donor age or to the time between the death of the donor and the primary cultivation.

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Section: Commentsmentioning

confidence: 79%

Post‐operative Thickness and Endothelial Cell Density in Cultivated, Cryopreserved Human Corneal Grafts

Ehlers

Sperling

Olsen

1982

Acta Ophthalmologica

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“…Central corneal thickness (CCT) measurements are valuable in the course and outcome of some diseases (6). Interactions between the factors that affect corneal endothelial function also affect its thickness.…”

Section: Introductionmentioning

confidence: 99%

Increased Corneal Thickness in Active Behçet's Disease

Evereklioğlu

2002

European Journal of Ophthalmology

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Behçet's patients with active ocular involvement have a thicker CCT than patients with inactive ocular involvement. There was no difference in CCT between controls and patients with no ocular involvement. The CCT of patients with active ocular involvement returned to nearly normal after treatment. CCT must be taken into account when developing a managing and following approach for Behcet's patients with active ocular involvement.

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“…All authors advocated rapid thawing of the frozen tis sues. Some authors have reported good clin ical results [Irvine et al, 1969;Mathieu, 1973], A major drawback of this technique is the variability of results, which can be attrib uted to minor variations in method. Post mortem time has been recognised to be an important prognostic factor for cell survival after cryotrauma.…”

Section: Introductionmentioning

confidence: 99%

A Simple Method for Controlled Freezing of Human Donor Corneae

Böhnke¹,

Draeger²,

Rethwisch³

et al. 1986

Ophthalmic Res

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A simple method for freezing cultured human donor material with dimethyl sulfoxide (DMSO) was developed empirically in a commercially available microcomputer-controlled apparatus allowing reproducible freezing curves to be made. The optimum cooling rate was found to be 1.0 °C/min down to –40 °C and then 5.0 °C/min to –80 °C. Total cell loss rates were 36.9 ± (SD) 19.8% of postmortem endothelial cell counts. An improved method for evaluating endothelial viability was applied, which enabled us to observe definite cell survival after a postculture period.

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Corneal Thickness: Postoperative Changes in Refrigerated and Cryopreserved Corneal Grafts

Cited by 23 publications

References 5 publications

Post‐operative Thickness and Endothelial Cell Density in Cultivated, Cryopreserved Human Corneal Grafts

Post‐operative Thickness and Endothelial Cell Density in Cultivated, Cryopreserved Human Corneal Grafts

Increased Corneal Thickness in Active Behçet's Disease

A Simple Method for Controlled Freezing of Human Donor Corneae

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