Coronal dentin differs between young and mature adult humans: A systematic review

Weerakoon, A.; Meyers, Ian; Thomson, David; Cooper, Crystal; Ford, Pauline; Symons, A. L.

doi:10.1016/j.archoralbio.2022.105553

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…Furthermore, the design of these surfaces in 4 mm diameter PDMS discs allows for its placement inside 96-well plates to carry out high-throughput experiments ranging from oral biofilm formation to potential tooth-biomaterial adhesive characterization, amongst others. Also, we have shown that it is possible to further tailor the mechanical and biological properties of these constructs (i.e., via glycation with relevant biological molecules) to study relevant processes in-vitro such as tooth aging and/or the effect of chronic hyperglycemia on dentinal properties 45,46 . Despite many advances in recent years, many questions remain regarding the pathogenesis of dental caries, particularly associated with vulnerable groups such as the elderly.…”

Section: Resultsmentioning

confidence: 99%

“…to study relevant processes in-vitro such as tooth aging and/or the effect of chronic hyperglycemia on dentinal properties 45,46 . Despite many advances in recent years, many questions remain regarding the pathogenesis of dental caries, particularly associated with vulnerable groups such as the elderly.…”

Section: Toward An In-vitro Biomimetic Dentin Microfabricated Substratementioning

confidence: 99%

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Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging

Álvarez,

Morales,

Tiozzo-Lyon

et al. 2023

Preprint

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Human dentin is a highly organized dental tissue displaying a complex microarchitecture consisting of micrometer-sized tubules encased in a mineralized type-I collagen matrix. As such, it serves as an important substrate for the adhesion of microbial colonizers and oral biofilm formation in the context of dental caries disease, including root caries in the elderly. Despite this issue, there remains a current lack of effective biomimetic in-vitro dentin models that facilitate the study of oral microbial adhesion by considering the surface architecture at the micro- and nanoscales. Therefore, the aim of this study was to develop a novel in-vitro microfabricated biomimetic dentin surface that simulates the complex surface microarchitecture of exposed dentin. For this, a combination of soft lithography microfabrication and biomaterial science approaches were employed to construct a micropitted PDMS substrate functionalized with mineralized type-I collagen. These dentin analogues were subsequently glycated with methylglyoxal (MGO) to simulate dentin matrix aging in-vitro and analyzed utilizing an interdisciplinary array of techniques including atomic force microscopy (AFM), elemental analysis, and electron microscopy. AFM force-mapping demonstrated that the nanomechanical properties of the biomimetic constructs were within the expected biological parameters, and that mineralization was mostly predominated by hydroxyapatite deposition. Finally, dual-species biofilms of Streptococcus mutans and Candida albicans were grown and characterized on the biofunctionalized PDMS microchips, demonstrating biofilm specific morphologic characteristics and confirming the suitability of this model for the study of early biofilm formation under controlled conditions. Overall, we expect that this novel biomimetic dentin model could serve as an in-vitro platform to study oral biofilm formation or dentin-biomaterial bonding in the laboratory without the need for animal or human tooth samples in the future.

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Section: Resultsmentioning

confidence: 99%

Section: Toward An In-vitro Biomimetic Dentin Microfabricated Substratementioning

confidence: 99%

Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging

Álvarez,

Morales,

Tiozzo-Lyon

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Also, we have shown that it is possible to further tailor the mechanical and biological properties of these constructs (i.e., via glycation with relevant biological molecules) to study relevant processes in vitro such as tooth aging and/or the effect of chronic hyperglycemia on dentinal properties. 45,46 Despite many advances in recent years, many questions remain regarding the pathogenesis of dental caries, particularly associated with vulnerable groups such as the elderly. Therefore, current dental research should focus on understanding how age-associated changes such as AGE accumulation within dental and periodontal tissues can promote disease.…”

Section: Toward An In Vitro Biomimetic Dentin Microfabricated Substratementioning

confidence: 99%

Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging

Álvarez,

Morales,

Tiozzo-Lyon

et al. 2024

Lab Chip

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“…Of note, dentin features and mechanical properties tend to change with age, with alterations occurring spatially within teeth depending on tubule orientation. Age-related mineral deposition within tubules and collagen maturation in intertubular dentin can markedly compound the spatial effects on mechanical properties of dentin [ 78 ]. The dentin tubules are enriched with minerals, the lumen disappear gradually (‘occlusion’) and the dentin around the tubules increases with age.…”

Section: Dental Aging-related Anatomical Changesmentioning

confidence: 99%

A New Landscape of Human Dental Aging: Causes, Consequences, and Intervention Avenues

Xie¹,

Chen²,

Shen³

et al. 2022

Aging and disease

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Aging is accompanied by physical dysfunction and physiologic degeneration that occurs over an individual's lifetime. Human teeth, like many other organs, inevitably undergo chronological aging and agerelated changes throughout the lifespan, resulting in a substantial need for preventive, restorative as well as periodontal dental care. This is particularly the case for seniors at 65 years of age and those older but economically disadvantaged. Dental aging not only interferes with normal chewing and digestion, but also affects daily appearance and interpersonal communications. Further dental aging can incur the case of multiple disorders such as oral cancer, encephalitis, and other systemic diseases. In the next decades or even hundreds of years, the proportion of the elderly in the global population will continue to rise, a tendency that attracts increasing attention across multiple scientific and medical disciplines. Dental aging will bring a variety of problems to the elderly themselves and poses serious challenges to the medical profession and social system. A reduced, but functional dentition comprising 20 teeth in occlusion has been proposed as a measurement index of successful dental aging. Healthy dental aging is critical to healthy aging, from both medical and social perspectives. To date, biomedical research on the causes, processes and regulatory mechanisms of dental aging is still in its infancy. In this article, updated insights into typical manifestations, associated pathologies, preventive strategies and molecular changes of dental aging are provided, with future research directions largely projected.

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Coronal dentin differs between young and mature adult humans: A systematic review

Cited by 6 publications

References 32 publications

Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging

Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging

Microfabrication-based engineering of biomimetic dentin-like constructs to simulate dental aging

A New Landscape of Human Dental Aging: Causes, Consequences, and Intervention Avenues

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