Correction: Kicking against the PRCs - A Domesticated Transposase Antagonises Silencing Mediated by Polycomb Group Proteins and Is an Accessory Component of Polycomb Repressive Complex 2

Liang, Shih Chieh; Hartwig, Ben; Perera, Pumi; Mora-Garcı́a, Santiago; Leau, Erica de; Thornton, Harry; Alves, Flávia de Lima; Rappsilber, Juri; Yang, Suxin; James, Geo Velikkakam; Schneeberger, Korbinian; Finnegan, E. Jean; Turck, Franziska; Goodrich, Justin

doi:10.1371/journal.pgen.1005812

Cited by 3 publications

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“…[ 23,29 ] Unlike AtRING1/AtBMI1‐harboring PRC1 has H2Aub activity, [ 31,32 ] the H2Aub levels did not change in emf1‐2 mutants. [ 32,33 ] Instead, recent data showed that EMF1 co‐purifies with PRC2 [ 34–36 ] and is required for H3K27me3 marking. [ 29,37,38 ] Nevertheless, EMF1 physically associates with components of both PRC1 and PRC2, [ 29,35 ] playing a vital role through H3K27me3 as well as histone 3 lysine4 trimethylation (H3K4me3) in the PcG‐mediated floral and seed developmental repression mechanism to allow vegetative growth and specify cell fates during growth and differentiation.…”

Section: Introductionmentioning

confidence: 99%

RNA 5‐Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in Arabidopsis

et al. 2022

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Methylating RNA post-transcriptionally is emerging as a significant mechanism of gene regulation in eukaryotes. The crosstalk between RNA methylation and histone modification is critical for chromatin state and gene expression in mammals. However, it is not well understood mechanistically in plants. Here, the authors report a genome-wide correlation between RNA 5-cytosine methylation (m 5 C) and histone 3 lysine27 trimethylation (H3K27me3) in Arabidopsis. The plant-specific Polycomb group (PcG) protein EMBRYONIC FLOWER1 (EMF1) plays dual roles as activators or repressors. Transcriptome-wide RNA m 5 C profiling revealed that m 5 C peaks are mostly enriched in chromatin regions that lacked H3K27me3 in both wild type and emf1 mutants. EMF1 repressed the expression of m 5 C methyltransferase tRNA specific methyltransferase 4B (TRM4B) through H3K4me3, independent of PcG-mediated H3K27me3 mechanism. The 5-Cytosine methylation on targets is increased in emf1 mutants, thereby decreased the mRNA transcripts of photosynthesis and chloroplast genes. In addition, impairing EMF1 activity reduced H3K27me3 levels of PcG targets, such as starch genes, which are de-repressed in emf1 mutants. Both EMF1-mediated promotion and repression of gene activities via m 5 C and H3K27me3 are required for normal vegetative growth. Collectively, t study reveals a previously undescribed epigenetic mechanism of RNA m 5 C modifications and histone modifications to regulate gene expression in eukaryotes.

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Section: Introductionmentioning

confidence: 99%

RNA 5‐Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in Arabidopsis

et al. 2022

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The genomes and transposable elements in plants: are they friends or foes?

Kim

2017

Genes Genom

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ZmEMF1a is required for the maintainence of H2Aub and H3K27me3 modifications in maize kernel development

Zhou,

Li,

et al. 2024

Preprint

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Polycomb group (PcG) proteins can silence gene expression by modifying histones, such as H2Aub and H3K27me3, which is crucial for maintaining cell type and tissue-specific gene expression patterns. However, little is known about the impact of gene regulation by PcG proteins through H2Aub and H3K27me3 during maize kernel development.Here, we characterized a maize miniature seed mutant mn8, and map-based cloning revealed that Mn8 encodes a plant specific PcG protein, ZmEMF1a. Mutation in ZmEMF1a leads to significantly reduced kernel size and weight. Molecular analyses showed that ZmEMF1ainteracts with PRC1 component ZmRING1 and PRC2 subunit ZmMSI1, which is required for H2Aub and H3K27me3 establishment. ZmEMF1a deficiency causes significant reduced levels of H2Aub and H3K27me3 in the genome. The combined analysis of ChIP-seq and RNA-seq data revealed that H2Aub is negatively correlated with gene expression in maize, unlike the positive association with expression of H2Aub in Arabidopsis. Compared with WT endosperms, elevated expressions of homology genes of cell proliferation repressors, such as DA1, BB1, ES22, MADS8 and MADS14, accompanied with decreases in H3K27me3 or H2Aub levels at these loci in mn8endosperms, indicating that lack of ZmEMF1a function impedes the deposition of H3K27me3 or H2Aub mark at cell division repressor genes. Taken together, our results show that ZmEMF1a plays a crucial role in regulating the expression of genes associated with maize kernel development through maintaining the modification levels of H2Aub and H3K27me3.

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Correction: Kicking against the PRCs - A Domesticated Transposase Antagonises Silencing Mediated by Polycomb Group Proteins and Is an Accessory Component of Polycomb Repressive Complex 2

Cited by 3 publications

References 0 publications

RNA 5‐Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in Arabidopsis

RNA 5‐Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in Arabidopsis

The genomes and transposable elements in plants: are they friends or foes?

ZmEMF1a is required for the maintainence of H2Aub and H3K27me3 modifications in maize kernel development

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