RNA 5‐Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in <i>Arabidopsis</i>

Zhang, Daolei; Guo, Weijun; Wang, Ting; Wang, Yifan; Le, Liang; Xu, Fan; Wu, Yue; Wuriyanghan, Hada; Sung, Z. Renee; Pu, Li

doi:10.1002/advs.202204885

Cited by 8 publications

(6 citation statements)

References 82 publications

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“…The dynamic modulation of pseudouridine under changing conditions further emphasized the reversibility and dynamic nature of RNA modifications and the epitranscriptome (Begik et al., 2021; Schwartz et al., 2014; Sun et al., 2019). The crosstalk and interplays between epigenetics and epitranscriptomics were also suggested and discussed recently (Kan et al., 2022; Xu & Shen, 2022; Zhang, Guo et al., 2023). These novel findings provide new insights into plant developmental biology and plant–environment interactions.…”

Section: Introductionmentioning

confidence: 85%

Current technical advancements in plant epitranscriptomic studies

et al. 2023

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The growth and development of plants are the result of the interplay between the internal developmental programming and plant–environment interactions. Gene expression regulations in plants are made up of multi‐level networks. In the past few years, many studies were carried out on co‐ and post‐transcriptional RNA modifications, which, together with the RNA community, are collectively known as the “epitranscriptome.” The epitranscriptomic machineries were identified and their functional impacts characterized in a broad range of physiological processes in diverse plant species. There is mounting evidence to suggest that the epitranscriptome provides an additional layer in the gene regulatory network for plant development and stress responses. In the present review, we summarized the epitranscriptomic modifications found so far in plants, including chemical modifications, RNA editing, and transcript isoforms. The various approaches to RNA modification detection were described, with special emphasis on the recent development and application potential of third‐generation sequencing. The roles of epitranscriptomic changes in gene regulation during plant–environment interactions were discussed in case studies. This review aims to highlight the importance of epitranscriptomics in the study of gene regulatory networks in plants and to encourage multi‐omics investigations using the recent technical advancements.

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Section: Introductionmentioning

confidence: 85%

Current technical advancements in plant epitranscriptomic studies

et al. 2023

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“…ZmBELL10‐myc transgenic plants were used for ChIP assays as previously described (Xu et al ., 2018; Zhang et al ., 2022). The chromatin complexes were incubated with anti‐MYC antibody (1 : 10 000 (v/v); Abcam, Cambridge, UK).…”

Section: Methodsmentioning

confidence: 99%

ZmBELL10 interacts with other ZmBELLs and recognizes specific motifs for transcriptional activation to modulate internode patterning in maize

Song

Guo

et al. 2023

New Phytologist

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Summary Plant height is an important agronomic trait that affects crop yield. Elucidating the molecular mechanism underlying plant height regulation is also an important question in developmental biology. Here, we report that a BELL transcription factor, ZmBELL10, positively regulates plant height in maize (Zea mays). Loss of ZmBELL10 function resulted in shorter internodes, fewer nodes, and smaller kernels, while ZmBELL10 overexpression increased plant height and hundred‐kernel weight. Transcriptome analysis and chromatin immunoprecipitation followed by sequencing showed that ZmBELL10 recognizes specific sequences in the promoter of its target genes and activates cell division‐ and cell elongation‐related gene expression, thereby influencing node number and internode length in maize. ZmBELL10 interacted with several other ZmBELL proteins via a spatial structure in its POX domain to form protein complexes involving ZmBELL10. All interacting proteins recognized the same DNA sequences, and their interaction with ZmBELL10 increased target gene expression. We identified the key residues in the POX domain of ZmBELL10 responsible for its protein–protein interactions, but these residues did not affect its transactivation activity. Collectively, our findings shed light on the functions of ZmBELL10 protein complexes and provide potential targets for improving plant architecture and yield in maize.

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“…434 In addition, mRNA modification by m5C plays a key role in transcription, RNA stability, and translational regulation. 435 Evaluation of the modification patterns of 530 tumoral samples in clear cell renal cell carcinoma (ccRCC) revealed that m5C methylation benefits the regulation of ccRCC immune infiltration. 436 Moreover, mRNA-based synthesis of therapeutic proteins confers distinct advantages, such as highly controlled translational kinetics and a minimized risk of insertional mutagenesis.…”

Section: Chemical Modifications On Mrnamentioning

confidence: 99%

“…The use of the pseudouridine and m5C-modified RALA mRNA nanocomplex has been shown to enhance the potency of cytolytic T cell responses . In addition, mRNA modification by m5C plays a key role in transcription, RNA stability, and translational regulation . Evaluation of the modification patterns of 530 tumoral samples in clear cell renal cell carcinoma (ccRCC) revealed that m5C methylation benefits the regulation of ccRCC immune infiltration …”

Section: Chemical Modifications On Rna Molecules In Preclinical Researchmentioning

confidence: 99%

Chemically Modified Platforms for Better RNA Therapeutics

Shi,

Zhen,

Zhang

et al. 2024

Chem. Rev.

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RNA-based therapies have catalyzed a revolutionary transformation in the biomedical landscape, offering unprecedented potential in disease prevention and treatment. However, despite their remarkable achievements, these therapies encounter substantial challenges including low stability, susceptibility to degradation by nucleases, and a prominent negative charge, thereby hindering further development. Chemically modified platforms have emerged as a strategic innovation, focusing on precise alterations either on the RNA moieties or their associated delivery vectors. This comprehensive review delves into these platforms, underscoring their significance in augmenting the performance and translational prospects of RNA-based therapeutics. It encompasses an in-depth analysis of various chemically modified delivery platforms that have been instrumental in propelling RNA therapeutics toward clinical utility. Moreover, the review scrutinizes the rationale behind diverse chemical modification techniques aiming at optimizing the therapeutic efficacy of RNA molecules, thereby facilitating robust disease management. Recent empirical studies corroborating the efficacy enhancement of RNA therapeutics through chemical modifications are highlighted. Conclusively, we offer profound insights into the transformative impact of chemical modifications on RNA drugs and delineates prospective trajectories for their future development and clinical integration. CONTENTS1. Introduction 930 2. Current Status and Challenges of RNA Therapeutics in Clinics 933 2.1. Molecular Mechanisms and Clinical Research Progress of RNAs 934 2.1.1. ASO 934 2.1.2. siRNA 934 2.1.3. Aptamer 936 2.1.4. mRNA 936 2.1.5. RNA Therapeutics on the Cusp of Clinical Breakthroughs 938 2.2. Major Challenges of Current RNA Therapeutics in Clinics 940 2.

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RNA 5‐Methylcytosine Modification Regulates Vegetative Development Associated with H3K27 Trimethylation in Arabidopsis

Cited by 8 publications

References 82 publications

Current technical advancements in plant epitranscriptomic studies

Current technical advancements in plant epitranscriptomic studies

ZmBELL10 interacts with other ZmBELLs and recognizes specific motifs for transcriptional activation to modulate internode patterning in maize

Chemically Modified Platforms for Better RNA Therapeutics

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