Correction: Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in <i>Xenopus</i>

Grenfell, Andrew W.; Heald, Rebecca; Strzelecka, Magdalena

doi:10.1083/jcb.20160402908222016c

Cited by 6 publications

(5 citation statements)

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“…Cen-RNA also interacts directly with the Chromosome Passenger Complex (CPC), including Aurora-B kinase, which controls accurate attachment between kinetochores and spindle microtubules (Carmena et al, 2012). Recent studies revealed that Aurora-B kinase localization and function at centromeres are controlled by its RNA binding at centromeres during mitosis (Ideue et al, 2014;Jambhekar et al, 2014;Grenfell et al, 2016a;Blower, 2016;Kabeche et al, 2018). Despite these findings, which domain of Aurora-B interacts with RNA and how RNA may affect its kinase activity are still unknown.…”

Section: Centromere Transcription and Centromere Rnamentioning

confidence: 99%

Dynamic redistribution and inheritance of chromatin:RNA interactions during cell division

Sparago,

Watanabe,

Sharp

et al. 2023

Front. RNA Res.

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During interphase, the nucleus contains a multitude of transcripts that influence the function of chromatin and global structure of the nucleus. Nuclear transcripts include nascent mRNAs in the process of transcription and mRNA processing, spliceosomal RNAs which catalyze mRNA processing, rRNAs that are being transcribed and processed to assemble functional ribosomes, and sno- and scaRNAs that participate in rRNA processing and modification. In addition, there are long noncoding RNAs (lncRNA) that associate with chromatin to control gene expression, or can even influence locus function in the case of centromeres and telomeres. Most of our knowledge of the functions of nuclear RNAs come from studies of interphase cells when the nuclear envelope separates nuclear and cytoplasmic contents. However, during mitosis the nuclear envelope breaks down, resulting in the mixing of nuclear and cytoplasmic components. Much less is known about the regulation and function of nuclear RNAs during mitosis. In this review, we discuss the cell cycle-dependent localization of different categories of RNAs, how the trans-acting factors SAF-A and Ki-67 regulate mitotic RNA localization, and describe how select categories of RNAs are inherited from the previous cell cycle in G1.

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Section: Centromere Transcription and Centromere Rnamentioning

confidence: 99%

Dynamic redistribution and inheritance of chromatin:RNA interactions during cell division

Sparago,

Watanabe,

Sharp

et al. 2023

Front. RNA Res.

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“…In flies, transcription of repeat satellite III (SATIII), which spans megabases on the X-, 2nd-, and 3rd-chromosome centromeres of D. melanogaster, is required for accurate mitosis by ensuring the correct localization of CENP-A and centromere protein C (CENP-C) on all chromosomes (30). Finally, studies with human cells and Xenopus egg extracts demonstrated that transcriptional activity at centromeres is required to ensure correct CENP-C levels and CENP-A loading and accurate chromosome segregation (31)(32)(33).…”

Section: Centromere Transcriptionmentioning

confidence: 99%

“…In mouse cells, two long RNA precursors, of 2,000 nt and 4,000 nt, are processed into 120-nt cenRNAs by an unknown mechanism (51). Grenfell and colleagues (33) recently revealed that splicing is required for correct spindle and kinetochore assembly in Xenopus egg extracts. In that study, the authors show that the splicing machinery interacts with and promotes the processing of the frog centromere repeat 1 (fcr1) cenRNA precursor, which in turn is required for the accurate localization of CENP-C, Aurora-B, and MCAK (mitotic centromere-associated kinesin), key factors in orchestrating kinetochore function.…”

Section: Dual Role For Centromere Transcriptionmentioning

confidence: 99%

“…CENP-C plays a crucial role in stabilizing CENP-A nucleosomes and linking CENP-A chromatin with the microtubule-binding complexes of the kinetochore. Interestingly, several studies have reported a conserved RNA-binding activity for CENP-C (13,30,33,34,50). Indeed, the physical interaction of CENP-C with transcripts derived from centromere transcription is required for stable localization of CENP-C to the centromere in several different organisms (13,30,31,50).…”

Section: Influence Of Centromere Transcription On Centromere Protein mentioning

confidence: 99%

“…Work in both humans and Xenopus has shown that centromere transcription and cenRNAs are required for normal Aurora-B localization and activation (38,69). Moreover, Grenfell et al (33) revealed that cotranscriptional recruitment of the RNA processing machinery to the centromere is also required for both localization and activation of Aurora-B in Xenopus. Work from our lab has shown that the CPC interacts directly with RNA through binding sites in Aurora-B and Borealin both in vitro and in vivo (38,70).…”

Section: Influence Of Centromere Transcription On Centromere Protein mentioning

confidence: 99%

See 2 more Smart Citations

Centromere Biology: Transcription Goes on Stage

Perea-Resa

Blower

2018

Molecular and Cellular Biology

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Accurate chromosome segregation is a fundamental process in cell biology. During mitosis, chromosomes are segregated into daughter cells through interactions between centromeres and microtubules in the mitotic spindle. Centromere domains have evolved to nucleate formation of the kinetochore, which is essential for establishing connections between chromosomal DNA and microtubules during mitosis. Centromeres are typically formed on highly repetitive DNA that is not conserved in sequence or size among organisms and can differ substantially between individuals within the same organism. However, transcription of repetitive DNA has emerged as a highly conserved property of the centromere. Recent work has shown that both the topological effect of transcription on chromatin and the nascent noncoding RNAs contribute to multiple aspects of centromere function. In this review, we discuss the fundamental aspects of centromere transcription, i.e., its dual role in chromatin remodeling/CENP-A deposition and kinetochore assembly during mitosis, from a cell cycle perspective.

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