Correction of timing errors in photomultiplier tubes used in phase-modulation fluorometry

Lakowicz, Joseph R.; Cherek, Henryk; Balter, Aleksander

doi:10.1016/0165-022x(81)90012-9

Cited by 234 publications

(93 citation statements)

References 29 publications

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“…All lifetime measurements were obtained using POPOP in the reference cell. The POPOP lifetime was 1.35 ns [34]. The fluorescence was measured through a long-pass filter type RG 370 from Janos Technology (Townshend, VT) which showed negligible luminescence.…”

Section: Fluorescence Measurementsmentioning

confidence: 99%

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Fiorini

Valentino

Gläser

et al. 1988

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane The fluorescence decay of 1,6 diphenyl-l,3,5-hexatriene (DPH) has been used to characterize aspects of the erythrocyte membrane structure related to the microheterogeneity of the lipid bilayer. The DPH decay has been studied using frequency domain fluorometry and the data analyzed either by a model of discrete exponential components or a model that assumes a continuous distribution of lifetime values. The main intensity fraction was associated with a lifetime value centered at about 11 ns in the erythrocyte membrane, but a short component of very low fractional intensity had to be considered to obtain a good fit to the data. The lifetime value of the long component was insensitive to temperature, while the width of the distribution decreased with increasing temperature. In multilamellar liposomes prepared from phospholipids extracted from the erythrocytes, the long lifetime component showed a temperature dependence. The depletion of 27% of the cholesterol in the erythocyte membrane induced a broadening of the distribution, suggesting a homogenizing effect of cholesterol. This effect has also been detected in egg phosphatidylcholine at a very low cholesterol/phospholipid molar ratio. The role of cholesterol on membrane heterogeneity is discussed in relation to the effect of cholesterol on water penetration.

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Section: Fluorescence Measurementsmentioning

confidence: 99%

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Fiorini

Valentino

Gläser

et al. 1988

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

show abstract

“…The POPOP lifetime was 1.35 ns [15]. The fluorescence was measured through a long-pass filter type RG 370 from Janos Technology (Townshed, VT) which showed negligible luminescence.…”

Section: Methodsmentioning

confidence: 99%

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

Fiorini

Gratton

Curatola

1989

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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“…A large range of modulation frequencies was used, between 2 and 130 MHz. POPOP in absolute ethanol (1.35 ns lifetime) was used as a reference [22]. Emission was collected through a Corion LG 370 S filter; data were accumulated until the standard deviations of phase and modulation values at a given frequency were below 0.1 and 0.002, respectively.…”

Section: Fluorescence Measurementsmentioning

confidence: 99%

Phosphatidic acid affects structural organization of phosphatidylcholine liposomes. A study of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammonium-phenyl)-6-phenyl,1,3,5-hexatriene (TMA-DPH) fluorescence decay using distributional analysis

Zolese

Gratton

Curatola

1990

Chemistry and Physics of Lipids

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Correction of timing errors in photomultiplier tubes used in phase-modulation fluorometry

Cited by 234 publications

References 29 publications

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

Phosphatidic acid affects structural organization of phosphatidylcholine liposomes. A study of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammonium-phenyl)-6-phenyl,1,3,5-hexatriene (TMA-DPH) fluorescence decay using distributional analysis

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