Correction of wavelength-dependent laser fluence in swept-beam spectroscopic photoacoustic imaging with a hand-held probe

Kim, Min Woo; Jeng, Geng-Shi; O’Donnell, Matthew; Pelivanov, Ivan

doi:10.1016/j.pacs.2020.100192

Cited by 27 publications

(21 citation statements)

References 31 publications

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“…In our previous clinical study on the human calcaneus bone, wavelength-dependent light attenuation was partially addressed based on the bone model [ 25 ]. Previous studies from other groups have also indicated that spectral coloring can be eliminated using model-based or machine-learning-based methods [ [41] , [42] , [43] , [44] , [45] , [46] , [47] ]. Therefore, in future clinical studies, PA absorption curves affected by spectral coloring should be further studied and corrected.…”

Section: Discussionmentioning

confidence: 99%

Detection of collagen by multi-wavelength photoacoustic analysis as a biomarker for bone health assessment

Tian

Xie

et al. 2021

Photoacoustics

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Collagen is an important biomarker of osteoporosis progression. Noninvasive, multispectral, photoacoustic (PA) techniques use pulsed laser light to induce PA signals to facilitate the visualization of chemical components that are strongly related to tissue health. In this study, the feasibility of multi-wavelength PA (MWPA) measurement of the collagen in bone, using the wavelength range of 1300–1800 nm, was investigated. First, the feasibility of this approach for detecting the collagen content of bone was demonstrated by means of numerical simulation. Then, ex vivo experiments were conducted on both animal and human bone specimens with different bone densities using the MWPA method. The relative collagen content was extracted and compared with the results of micro-computed tomography (micro-CT) and histology. The results showed that the “relative collagen content” parameter obtained using the MWPA approach correlated well with the bone volume ratio obtained from micro-CT images and histological analysis results. This study highlights the potential of the proposed PA technique for determining the collagen content of bones as a biomarker for bone health assessment.

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Section: Discussionmentioning

confidence: 99%

Detection of collagen by multi-wavelength photoacoustic analysis as a biomarker for bone health assessment

Tian

Xie

et al. 2021

Photoacoustics

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“…The density of certain tissues or materials also affects the laser absorption in photoacoustic imaging. Chromophores are tissue components that can absorb laser light energy [20]. Several chromophores, such as hydroxyapatite, protein, and blood vessels can be found in oral tissue [21].…”

Section: (A) (B)mentioning

confidence: 99%

Photoacoustic Imaging for Periodontal Disease Examination

Mitrayana¹,

Sari²,

Widyaningrum³

2021

Preprint

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Inflammation of the periodontal tissue (periodontitis) is the highest problem of oral health in Indonesia after caries. Photoacoustic imaging (PAI) is a new imaging technique that can be simply constructed using a diode laser combined with a condenser microphone. This study aims to determine that a simple PAI system was able to image periodontal disease in animal model. Samples of the study were normal periodontal and periodontitis tissue, obtained from Sprague-Dawley rats that were divided into four groups, i.e. the control group, treatment group 1 (7 days periodontitis induction), treatment group 2 (11 days periodontitis induction), and treatment group 3 (14 days periodontitis induction). The PAI system was controlled by Labview and Arduino IDE software from a personal computer. Results of the study reveal that the optimal frequency of laser modulation for periodontal tissue imaging was 19 kHz with duty cycle of 50%. Photoacoustic (PA) intensity was obtained from higher to lower of -68,71 dB (treatment group 3), -70,69 dB (treatment group 2), -71,69 dB (treatment group 1), and -73,07 dB (control group) respectively. The photoacoustic images were analyzed to define the contrast between sample and media. The PA intensity of the samples were higher than media. Therefore, this study demonstrate the feasibility of simple PAI system to differentiate normal periodontal tissue and periodontitis.

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“…Kim et al have developed a real-time, spectroscopic PA/US system with simultaneous fluence compensation and motion correction. 149 For PA acquisition, a single image is formed by sequential scanning using a narrow laser beam through fiber sweeping (called “fast-sweep”). Light from different fibers propagates different distances in tissue.…”

Section: Technical Advancements In Molecular Patmentioning

confidence: 99%

“…The procedure is repeated for all wavelengths used in the experiment. Reproduced with permission 149.…”

mentioning

confidence: 99%

Sound Out the Deep Colors: Photoacoustic Molecular Imaging at New Depths

Nyayapathi

Kilian

et al. 2020

Mol Imaging

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Photoacoustic tomography (PAT) has become increasingly popular for molecular imaging due to its unique optical absorption contrast, high spatial resolution, deep imaging depth, and high imaging speed. Yet, the strong optical attenuation of biological tissues has traditionally prevented PAT from penetrating more than a few centimeters and limited its application for studying deeply seated targets. A variety of PAT technologies have been developed to extend the imaging depth, including employing deep-penetrating microwaves and X-ray photons as excitation sources, delivering the light to the inside of the organ, reshaping the light wavefront to better focus into scattering medium, as well as improving the sensitivity of ultrasonic transducers. At the same time, novel optical fluence mapping algorithms and image reconstruction methods have been developed to improve the quantitative accuracy of PAT, which is crucial to recover weak molecular signals at larger depths. The development of highly-absorbing near-infrared PA molecular probes has also flourished to provide high sensitivity and specificity in studying cellular processes. This review aims to introduce the recent developments in deep PA molecular imaging, including novel imaging systems, image processing methods and molecular probes, as well as their representative biomedical applications. Existing challenges and future directions are also discussed.

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Correction of wavelength-dependent laser fluence in swept-beam spectroscopic photoacoustic imaging with a hand-held probe

Cited by 27 publications

References 31 publications

Detection of collagen by multi-wavelength photoacoustic analysis as a biomarker for bone health assessment

Detection of collagen by multi-wavelength photoacoustic analysis as a biomarker for bone health assessment

Photoacoustic Imaging for Periodontal Disease Examination

Sound Out the Deep Colors: Photoacoustic Molecular Imaging at New Depths

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