“…Traditional methods such as visual surveys are very costly, time-consuming and require on-site taxonomic expertise (Kim and Byrne, 2006; Ballesteros-Mejia et al ., 2013; Dornelas et al ., 2019). Despite decades of sampling efforts, biodiversity monitoring still covers only a small fraction of global ecosystems and is particularly challenging in isolated and remote regions across the oceans (Collen et al ., 2009; Webb, Vanden Berghe and O’Dor, 2010; Dornelas et al ., 2018; Letessier et al ., 2019). An emerging tool for rapid biodiversity assessment is environmental DNA (eDNA) metabarcoding (Stat et al ., 2017;Eble et al ., 2020), which is proving to be particularly effective for marine environments (Juhel et al ., 2020; Boulanger et al ., 2021; Holman et al ., 2021).…”