Correction to: Detection of Fusobacterium nucleatum in stool and colonic tissues from Norwegian colorectal cancer patients

Tunsjø, Hege Smith; Gundersen, Gro; Rangnes, Fredrik; Noone, J Christopher; Endres, Alexander; Bemanian, Vahid

doi:10.1007/s10096-019-03712-x

Cited by 6 publications

(3 citation statements)

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“…As a result, there are restrictions to the techniques for isolating, cultivating, and identifying intestinal microbes. Currently, RT-qPCR and metagenome sequencing analysis for F. nucleatum in feces have a broad range of applications, and they have better accuracy and higher credibility . We need to develop a more practical detection technology for F. nucleatum to avert the drawbacks of the aforementioned techniques such as intricate processes and increased costs.…”

Section: Introductionmentioning

confidence: 99%

Whole-Bacterium SELEX Aptamer Selection of Fusobacterium nucleatum and Application to Colorectal Cancer Noninvasive Screening in Human Feces

Wang¹,

Fan

Chen

et al. 2023

Anal. Chem.

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In terms of cancer diagnoses and cancer-related deaths worldwide, colorectal cancer (CRC) is now the third most common malignancy. The drawbacks of current screening methods are their exorbitant costs, difficult procedures, and lengthy implementation timelines. The benefits of fecal screening for CRC are ease of operation, noninvasiveness, cost-effectiveness, and superior sensitivity. As a result of its enrichment in the malignant tissues and feces of CRC patients, Fusobacterium nucleatum (F. nucleatum) has emerged as a crucial biomarker for the incipient detection, identification, and prognostic prediction of CRC. Here, for the first time, the whole-bacterium SELEX method was used to screen the highly specific and affinity aptamers against F. nucleatum by 13 cycles of selection. The Apt-S-5 linear correlation equation is y = 0.7363x 2.8315 (R 2 = 0.9864) with a limit of detection (LOD) of 851 CFU/mL. The results of the experiment using fecal samples revealed a substantial disparity between the microorganisms in the CRC patients’ feces and those in the feces of healthy individuals and were consistent with those of qPCR. The aptamers may therefore offer a crucial approach to identifying F. nucleatum and hold tremendous promise for CRC diagnosis and prognostic prediction.

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Section: Introductionmentioning

confidence: 99%

Whole-Bacterium SELEX Aptamer Selection of Fusobacterium nucleatum and Application to Colorectal Cancer Noninvasive Screening in Human Feces

Wang¹,

Fan

Chen

et al. 2023

Anal. Chem.

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“…Some residents of microbiota in gut such as Fusobacterium spp., Escherichia coli and Bacteroides fragilis are suspected to play role in CRC 1 . Although there is a small amount of F. nucleatum found in the gut microbiota, it is supported that it may cause infection and consequently cancer in the colon tissue 4 .The microbial community in the colon may influence the immune system development of host through their metabolites such as butyrate and retinoic acid. F. nucleatum promotes CRC through several virulence mechanisms such as invasion and modulation of host immune response 5 .…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer

KURT>

Yumuk

2021

Sci Rep

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The colorectal cancer is a serious health problem. The diagnosis of the disease mostly relies on an invasive procedure. A non-invasive diagnostic test such as an immunoassay, may facilitate diagnosis of colorectal cancer. The purpose of the study was to evaluate the use of antibodies against Fusobacterium nucleatum in the diagnosis of colorectal cancer (CRC). Totally 78 patients in three groups were included in the study. F. nucleatum in the tissues was detected using quantitative polymerase chain reaction assay. F. nucleatum IgA and IgG were measured using enzyme linked immunosorbent assay. F. nucleatum was detected in 86.7% and 73.1% cases of CRC and precancerous-benign colon disease (P-BCD), respectively. The OD values from F. nucleatum IgA and IgG ELISA tests were higher in CRC group compared with healthy individuals. The sensitivity of IgA ELISA test varied between 31.8 and 95.5% depending on the chosen cut-off values. The positivity rate of antibodies in patients with high amount of F. nucleatum in tissue was significantly greater than in the negative group. The F. nucleatum IgA and IgG antibodies in CRC were higher than the ones in healthy controls but the discriminative ability of the ELISA test was not adequate to be considered as a diagnostic tool.

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Precision strategies for cancer treatment by modifying the tumor-related bacteria

Wang

Liu

et al. 2021

Appl Microbiol Biotechnol

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Correction to: Detection of Fusobacterium nucleatum in stool and colonic tissues from Norwegian colorectal cancer patients

Cited by 6 publications

References 0 publications

Whole-Bacterium SELEX Aptamer Selection of Fusobacterium nucleatum and Application to Colorectal Cancer Noninvasive Screening in Human Feces

Whole-Bacterium SELEX Aptamer Selection of Fusobacterium nucleatum and Application to Colorectal Cancer Noninvasive Screening in Human Feces

Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer

Precision strategies for cancer treatment by modifying the tumor-related bacteria

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