Correction to Inhibition of Quorum Sensing and Virulence in <i>Serratia marcescens</i> by Hordenine

Zhou, Jinwei; Ruan, Ling-Yu; Chen, Hong-Juan; Luo, Huai-Zhi; Jiang, Huan; Wang, Junsong; Jia, Ai‐Qun

doi:10.1021/acs.jafc.9b01005

Cited by 3 publications

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“… 47 Additionally, flagella-controlled swarming motility is governed by flhD and contributes to cell surface attachment. 18 , 48 In this study, all S. marcescens carried flhD gene, and 50.0% of the isolates were positive for fimA and fimC . Besides, we also found that all S. marcescens isolates harbored the adhesions protein-related virulence gene kpn , the majority of the isolates carried mrkD (93.8%), ycfM (81.3%) and bsmB (81.3%).…”

Section: Discussionmentioning

confidence: 50%

“…Prodigiosin is a prominent red pigment produced by S. marcescens and is essential for invasion, survival, and pathogenicity. 18 , 57 We tested the prodigiosin biosynthesis-related genes and found the most frequent gene was pigP (81.3%), followed by pigC (37.5%). PigP is a positive regulator of prodigiosin production that regulates swarming and hemolysis through serratamolide production.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Zhong¹,

Shen²,

Liu³

et al. 2023

IDR

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Purpose This study aimed to investigate the prevalence and genetic characterization of Serratia marcescens isolates from clinical bovine mastitis in Ningxia Hui Autonomous Region of China. Methods S. marcescens was identified by the polymerase-chain reaction of 16S rRNA gene and sequencing. Antimicrobial susceptibility was tested by the disk diffusion method. Genes of resistance and virulence were determined by the PCR. Results Overall, S. marcescens were confirmed from 32 of 2897 (1.1%) mastitis milk samples. These isolates showed high resistance to cefazolin (30/32, 93.8%) and chloramphenicol (28/32, 87.5%). A 12.5% (4/32) of the isolates displayed multidrug resistance (MDR). The most prevalent resistant genes found in S. marcescens were TEM (32/32, 100%) and CTX-M (24/32, 75.0%; CTX-M-15 , 14/32, 43.8%; CTX-M-14 , 8/32, 25.0%; CTX-M-65 , 2/32, 6.3%) for extended-spectrum beta-lactamase, cmlA (28/32, 87.5%) and floR (16/32, 50.0%) for chloramphenicol resistance, SIM-1 (2/32, 6.3%) for carbapenemases, and sdeB (28/32, 87.5%), sdeY (26/32, 81.3%), sdeR (26/32, 81.3%) and sdeD (20/32, 62.5%) for efflux pumps. Moreover, all isolates carried virulence genes flhD, entB , and kpn , and most of them contained mrkD (30/32, 93.8%), ycfM (26/32, 81.3%), bsmB (26/32, 81.3%), pigP (26/32, 81.3%), kfu (24/32, 75.0%) and shlB (24/32, 75.0%). Conclusion To our knowledge, this is the first report of genetic determinants for antimicrobial resistance and virulence in S. marcescens isolated from bovine mastitis cases in China. These findings are useful for developing strategies for prevention and treatment of bovine mastitis caused by S. marcescens in China.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Zhong¹,

Shen²,

Liu³

et al. 2023

IDR

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“…The quantitative real-time PCR (qRT-PCR) analysis was performed to assess the mechanisms underlying the inhibitory effects of 3-PPA on biofilms and virulence factors. We analyzed the effects of 3-PPA on the transcript levels of fimA , fimC , pigP , flC , flhD , bmsB , and sodB genes, which are involved in S. marcescens NJ01 hyphal production, adhesion, motility, and biofilm formation ( 19 ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

et al. 2022

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Discovery of β‐nitrostyrene derivatives as potential quorum sensing inhibitors for biofilm inhibition and antivirulence factor therapeutics against Serratia marcescens

Wang,

Yang,

Durairaj

et al. 2024

mLife

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Quorum sensing (QS) inhibition has emerged as a promising target for directed drug design, providing an appealing strategy for developing antimicrobials, particularly against infections caused by drug‐resistant pathogens. In this study, we designed and synthesized a total of 33 β‐nitrostyrene derivatives using 1‐nitro‐2‐phenylethane (NPe) as the lead compound, to target the facultative anaerobic bacterial pathogen Serratia marcescens. The QS‐inhibitory effects of these compounds were evaluated using S. marcescens NJ01 and the reporter strain Chromobacterium violaceum CV026. Among the 33 new β‐nitrostyrene derivatives, (E)‐1‐methyl‐4‐(2‐nitrovinyl)benzene (m‐NPe, compound 28) was proven to be a potent inhibitor that reduced biofilm formation of S. marcescens NJ01 by 79%. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) results revealed that treatment with m‐NPe (50 μg/ml) not only enhanced the susceptibility of the formed biofilms but also disrupted the architecture of biofilms by 84%. m‐NPe (50 μg/ml) decreased virulence factors in S. marcescens NJ01, reducing the activity of protease, prodigiosin, and extracellular polysaccharide (EPS) by 36%, 72%, and 52%, respectively. In S. marcescens 4547, the activities of hemolysin and EPS were reduced by 28% and 40%, respectively, outperforming the positive control, vanillic acid (VAN). The study also found that the expression levels of QS‐ and biofilm‐related genes (flhD, fimA, fimC, sodB, bsmB, pigA, pigC, and shlA) were downregulated by 1.21‐ to 2.32‐fold. Molecular dynamics analysis showed that m‐NPe could bind stably to SmaR, RhlI, RhlR, LasR, and CviR proteins in a 0.1 M sodium chloride solution. Importantly, a microscale thermophoresis (MST) test revealed that SmaR could be a target protein for the screening of a quorum sensing inhibitor (QSI) against S. marcescens. Overall, this study highlights the efficacy of m‐NPe in suppressing the virulence factors of S. marcescens, identifying it as a new potential QSI and antibiofilm agent capable of restoring or improving antimicrobial drug sensitivity.

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Correction to Inhibition of Quorum Sensing and Virulence in Serratia marcescens by Hordenine

Cited by 3 publications

References 0 publications

Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

Discovery of β‐nitrostyrene derivatives as potential quorum sensing inhibitors for biofilm inhibition and antivirulence factor therapeutics against Serratia marcescens

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