Correlating the immune response with the clinical–pathological course of persistent mastitis experimentally induced by Mycoplasma agalactiae in dairy goats

Castro-Alonso, Ayoze; Rodrı́guez, Francisco; Fe, Christian de la; Monteros, A. Espinosa de los; Andrada, M.; Herráez, P.

doi:10.1016/j.rvsc.2008.06.004

Cited by 31 publications

(47 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antibiotic treatment would provide only a limited and transient recovery but would not provide a long-term solution; it is also expensive, runs the risk of leading to antimicrobial resistance and milk withdrawal periods. Experimental results confirm the above scenarios with Castro-Alonso and others8 reporting mean titres of 10 9 cfu/ml after 45 days after intrammary infection of goats and Sanchis and others9 reporting 10 5 cfu/ml after 49 days in sheep.…”

Section: Discussionsupporting

confidence: 83%

Can vaccines for contagious agalactia reduce disease progression in infected animals: a preliminary study?

Loria

Puleio

Agnello

et al. 2018

Vet Record Case Reports

View full text Add to dashboard Cite

A flock of sheep in Central Sicily was affected by a severe outbreak of contagious agalactia (CA) caused by Mycoplasma agalactiae, affecting nearly 30% resulting in a large drop in milk production. Many ewes had warm and swollen udders that often became sclerotic and was accompanied by keratoconjunctivitis and arthritis in both adults and young. As antibiotic treatment appeared ineffective, the whole flock was given two doses of an inactivated vaccine against M agalactiae two weeks apart. A small group was selected for close examination and additional tests. A fortnight after the last vaccination mycoplasma excretion fell in all but one of the selected ewes and was undetectable in all but two animals two weeks after that. In nearly all the selected ewes, there was an improvement in milk quality and udder condition. This work provided preliminary evidence for the continued use of CA vaccines to slow or prevent disease progression.

show abstract

Section: Discussionsupporting

confidence: 83%

Can vaccines for contagious agalactia reduce disease progression in infected animals: a preliminary study?

Loria

Puleio

Agnello

et al. 2018

Vet Record Case Reports

View full text Add to dashboard Cite

show abstract

“…Only Gov2 presented a response after the challenge, achieving the peak from the 14 th to the 28 th day, when they initiated a reduction in antibodies (Figure 4). These results are similar to Castro-Alonso et al (2009) who analyzed the immunological response of goats experimentally infected though intramammary use and then verified that the anti-Ma antibodies were presented on the 11th day after infection and persisted until the 32nd day. According to Hasso and Al-Omran (1994), the inoculation way might interfere on the antibodies production time against Ma.…”

Section: Caprinesupporting

confidence: 88%

Efficiency of inactive vaccines against contagious agalactia in Brazil

Campos

Azevedo

Alcântara

et al. 2013

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

This paper aims to evaluate the efficiency of three inactive vaccines against contagious agalactia prepared with samples of Mycoplasma agalactiae isolated in Brazil and different adjuvants. Vaccine 1 adsorbed with aluminum hydroxide was administered in 23 goats (Gc1) and 13 sheep (Gov1); vaccine 2 containing Montanide IMS-2215-VG was administered in 22 goats (Gc2) and 12 sheep (Gov2) and vaccine 3, containing Montanide Gel-01 was administered in 22 goats (Gc3) and 12 sheep (Gov3). All animals were negative for Ma at indirect ELISA and received two doses of 2mL each, subcutaneously, within a 21 day interval. Five animals from each species were used as control. Seventy-five days after the booster, four animals from each vaccinated group and two from the control group were challenged with 5mL of Ma culture containing 10 7 cfu/mL, orally and through immersion of the female's udder in lactation. The serological response was analyzed during vaccination days (0 and 21) and at 51, 81, 111, 141 and 171 days after vaccination. The collection and analysis of the challenged animals were conducted at the day of the challenge (D0) and 7, 14, 21, 28, 35, 42, 49 and 56 days after the challenge. The three vaccines induced the production of antibodies, having no significant statistical difference (p<0.05). Animals from groups Gc1, Gc2 and Gov2 developed higher levels of antibodies, with significant statistical difference compared to the other vaccinated group and control group (p<0.05). After the challenge, the animals from the control presented an increase in regional lymph nodes and conjunctivitis, mastitis and arthritis. In four vaccinated animals, discrete conjunctivitis and congestion of the episcleral veins was observed. It is concluded that vaccines 1 and 2 induced levels of protective antibodies in goats and sheep, sufficient for clinical protection of the animals submitted to the experimental infection, indicating its use on the prevention of contagious agalactia.Keywords: Mycoplasma agalactiae, goats, sheep, immunization, ELISA RESUMO Este trabalho tem como objetivo avaliar a eficácia de três vacinas inativadas contra agalaxia contagiosa, preparadas com amostra de Mycoplasma agalactiae isolada no Brasil e diferentes adjuvantes. A vacina 1, adsorvida com hidróxido de alumínio, foi administrada em 23 caprinos (Gc1) e 13 ovinos (Gov1); a vacina 2, contendo Montanide IMS-2215-VG, foi administrada em 22 caprinos (Gc2) e 12 ovinos (Gov2); e a vacina 3, contendo Montanide Gel-01 foi administrada em 22 caprinos (Gc3) e 12 ovinos (Gov3). Todos os animais eram negativos para

show abstract

“…agalactiae has also been previously reported to induce a strong innate immune response involving neutrophils and macrophages in the first few days of infection in goats, subsequently followed by a specific antibody response by Day 7 p.i. that coincided with reduced viable mycoplasma counts in milk, but showed this humoral response to be incapable of controlling mycoplasma invasion [4]. …”

Section: Discussionmentioning

confidence: 99%

“…With respect to the involved host factors, except for a couple of immune response reports, that too in goats, where under natural conditions four other mycoplasma species are known to cause clinically indistinguishable syndrome [4, 18], the dynamics of M . agalactiae induced host responses in the mastitic sheep mammary gland are almost completely unknown.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive RNA-Seq Profiling to Evaluate the Sheep Mammary Gland Transcriptome in Response to Experimental Mycoplasma agalactiae Infection

et al. 2017

View full text Add to dashboard Cite

Mycoplasma agalactiae is a worldwide serious pathogen of small ruminants that usually spreads through the mammary route causing acute to subacute mastitis progressing to chronic persistent disease that is hard to eradicate. Knowledge of mechanisms of its pathogenesis and persistence in the mammary gland are still insufficient, especially the host-pathogen interplay that enables it to reside in a chronic subclinical state. This study reports transcriptome profiling of mammary tissue from udders of sheep experimentally infected with M. agalactiae type strain PG2 in comparison with uninfected control animals using Illumina RNA-sequencing (RNA-Seq). Several differentially expressed genes (DEGs) were observed in the infected udders and RT-qPCR analyses of selected DEGs showed their expression profiles to be in agreement with results from RNA-Seq. Gene Ontology (GO) analysis revealed majority of the DEGs to be associated with mycoplasma defense responses that are directly or indirectly involved in host innate and adaptive immune responses. Similar RNA-Seq analyses were also performed with spleen cells of the same sheep to know the specific systemic transcriptome responses. Spleen cells exhibited a comparatively lower number of DEGs suggesting a less prominent host response in this organ. To our knowledge this is the first study that describes host transcriptomics of M. agalactiae infection and the related immune-inflammatory responses. The data provides useful information to further dissect the molecular genetic mechanisms underlying mycoplasma mastitis, which is a prerequisite for designing effective intervention strategies.

show abstract

Correlating the immune response with the clinical–pathological course of persistent mastitis experimentally induced by Mycoplasma agalactiae in dairy goats

Cited by 31 publications

References 28 publications

Can vaccines for contagious agalactia reduce disease progression in infected animals: a preliminary study?

Can vaccines for contagious agalactia reduce disease progression in infected animals: a preliminary study?

Efficiency of inactive vaccines against contagious agalactia in Brazil

Comprehensive RNA-Seq Profiling to Evaluate the Sheep Mammary Gland Transcriptome in Response to Experimental Mycoplasma agalactiae Infection

Contact Info

Product

Resources

About