Correlation Analysis Between Gene Expression Profile of Rat Liver Tissues and High-Fat Emulsion-Induced Nonalcoholic Fatty Liver

Xu, Chi; Wang, Gaiping; Hao, Yunpeng; Zhi, Jia; Zhang, Lianxing; Chang, Cuifang

doi:10.1007/s10620-011-1599-9

Cited by 34 publications

(31 citation statements)

References 37 publications

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“…We also investigated the main target of RK in NASH. 32,33 The results assist in the development of a safe and effective natural intervention for NASH.…”

Section: Introduction Rmentioning

confidence: 94%

Raspberry Ketone Protects Rats Fed High-Fat Diets Against Nonalcoholic Steatohepatitis

Wang

Meng

Zhang

2012

Journal of Medicinal Food

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The protective effect of raspberry ketone against nonalcoholic steatohepatitis (NASH) was tested by using a high-fat diet-induced NASH model, and its mechanism was explored. Forty Sprague-Dawley rats with a 1:1 male to female ratio were randomly divided into five groups: the normal control (NC) group (n = 8) fed normal diet for 8 weeks, the model control (MC) group (n = 8) fed high-fat diet (82% standard diet, 8.3% yolk powder, 9.0% lard, 0.5% cholesterol, and 0.2% sodium taurocholate), and the raspberry ketone low-dose (0.5%) (RKL) group (n = 8), the raspberry ketone middle-dose (1%) (RKM) group (n = 8), and the raspberry ketone high-dose (2%) (RKH) group (n = 8) fed high-fat diet for 4 weeks. were measured in rats. Therefore, we determined the peroxisome proliferator-activated receptor (PPAR)-a activity in liver homogenate and the levels of low-density lipoprotein receptor (LDLR), high-sensitivity C-reactive protein (hs-CRP), adiponection (APN), superoxide dismutase, and malondialdehyde (MDA). The liver tissues of rats in each group were imaged by electron microscopy with hematoxylin-eosin as the staining agent. The levels of TG, TC, LDL-C, ALT, AST, ALP, GLU, INS, IRI, FFA, LEP, TNF-a, MDA, and hs-CRP of MC rats were significantly increased (P < .05, P < .01). Therefore, the levels of HDL-C, ISI, PPAR-a, LDLR, and APN were significantly decreased (P < .05, P < .01). Compared with the MC group, each parameter in the RKL, RKM, and RKH groups was significantly improved (P < .05, P < .01). Thus raspberry ketone was an effective intervention for NASH in rats. It was believed that raspberry ketone had a dual effect of liver protection and fat reduction, and the mechanism was probably mediated by alleviation of fatty degeneration of liver cells, decreased liver inflammation, correction of dyslipidemia, reversal of LEP and INS resistance, and improved antioxidant capacity.

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“…We also investigated the main target of RK in NASH. 32,33 The results assist in the development of a safe and effective natural intervention for NASH.…”

Section: Introduction Rmentioning

confidence: 94%

Raspberry Ketone Protects Rats Fed High-Fat Diets Against Nonalcoholic Steatohepatitis

Wang

Meng

Zhang

2012

Journal of Medicinal Food

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“…Emerging evidence suggests the importance of the epigenetic machinery coupled with changes of gene expression profile in the regulation of NASH pathogenesis. For example, a genome-wide association study revealed genes involved in cellular apoptosis, lipid biosynthesis, and inflammation response increase during NASH progression, whereas those involved in DNA damage response signal transduction, cholesterol biosynthesis, and carbohydrate metabolism decrease [32]. Another study found that mitochondrially encoded NADH dehydrogenase 6 (MT-ND6) is hypermethylated in human patients with NAFLD and the methylation status is associated with the histological severity of NAFLD [33].…”

Section: Genetic and Epigenetic Regulation In Nashmentioning

confidence: 99%

Pathogenesis of Nonalcoholic Steatohepatitis: Interactions between Liver Parenchymal and Nonparenchymal Cells

Magee

Zou

Zhang

2016

BioMed Research International

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Nonalcoholic fatty liver disease (NAFLD) is the most common type of chronic liver disease in the Western countries, affecting up to 25% of the general population and becoming a major health concern in both adults and children. NAFLD encompasses the entire spectrum of fatty liver disease in individuals without significant alcohol consumption, ranging from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH) and cirrhosis. NASH is a manifestation of the metabolic syndrome and hepatic disorders with the presence of steatosis, hepatocyte injury (ballooning), inflammation, and, in some patients, progressive fibrosis leading to cirrhosis. The pathogenesis of NASH is a complex process and implicates cell interactions between liver parenchymal and nonparenchymal cells as well as crosstalk between various immune cell populations in liver. Lipotoxicity appears to be the central driver of hepatic cellular injury via oxidative stress and endoplasmic reticulum (ER) stress. This review focuses on the contributions of hepatocytes and nonparenchymal cells to NASH, assessing their potential applications to the development of novel therapeutic agents. Currently, there are limited pharmacological treatments for NASH; therefore, an increased understanding of NASH pathogenesis is pertinent to improve disease interventions in the future.

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“…After that, total RNA from liver tissues at 0, 3, 6 and 9 weeks of rat model of LC were applied for microarray analysis using Rat Genome 230 2.0 Array (Affymetrix Inc., Santa Clara, CA, USA). To minimize technical errors in the array analyses, the liver sample was detected repeatedly for three times at each time point by Rat Genome 230 2.0 Array, totaling 3 arrays × 4 time points (Xu et al, 2011).…”

Section: Rat Genome 230 20 Microarray Detectionmentioning

confidence: 99%

Relationship analysis between gene expression profiles and rat liver cirrhosis occurrence

Wang¹,

Zhao²,

Chen³

et al. 2017

Afr. J. Biotechnol.

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Liver cirrhosis (LC) is a kind of liver disease which is pathologically characterized by abnormality and necrosis of hepatic cells, proliferation of fibrous tissue, nodular regeneration and pseudolobule formation. To explore the mechanism of LC occurrence at the level of mRNA, Rat Genome 230 2.0 Array was used to detect gene expression profiles of rat fibrotic livers in 3, 6 and 9 weeks after CCl 4 treatment in this study. It was found that a total of 305 genes including 153 up-regulated, 150 down-regulated and 2 up/down-regulated genes, were related to LC occurrence. Then, k-means clustering was employed to classify above 305 genes into 5 clusters based on gene expression similarity, and EASE analysis further indicated that the above genes were mainly associated with metabolic process, stress reaction, cell growth and apoptosis/death. Thereafter, ingenuity pathway analysis (IPA) software was used to analyze potential effects of the above-mentioned 305 genes, and the results suggested that lipid metabolism and cell growth were inhibited while cell apoptosis/death was activated, but immune/inflammatory response was first activated and then inhibited. Furthermore, IPA also predicted that several signal pathways "ERK/MAPK Signaling", "p38 MAPK", "Endothelin-1 Signaling", "Growth Hormone Signaling", "LPS/IL-1-mediated inhibition of RXR function" and "IL-6 Signaling" were involved in regulating the occurrence of liver cirrhosis. It was concluded that 305 genes and 3 kinds of physiological activities were closely related to LC occurrence.

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Correlation Analysis Between Gene Expression Profile of Rat Liver Tissues and High-Fat Emulsion-Induced Nonalcoholic Fatty Liver

Abstract: These data provide useful information on the global gene expression changes due to high-fat emulsion feeding and bring important insights into the mechanisms of NAFLD.

Cited by 34 publications

References 37 publications

Raspberry Ketone Protects Rats Fed High-Fat Diets Against Nonalcoholic Steatohepatitis

Raspberry Ketone Protects Rats Fed High-Fat Diets Against Nonalcoholic Steatohepatitis

Pathogenesis of Nonalcoholic Steatohepatitis: Interactions between Liver Parenchymal and Nonparenchymal Cells

Relationship analysis between gene expression profiles and rat liver cirrhosis occurrence

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