Correlation analysis of serum reproductive hormones and metabolites during multiple ovulation in sheep

Xu, Qiwu; Wang, Chunwei; Wang, Lequn; Feng, Rui; Guo, Yi; Feng, Shi Ting; Zhang, Liguo; Zheng, Zhong; Su, Guanghua; Fan, Lifen; Bian, Chao; Zhang, Li; Su, Xianwei

doi:10.1186/s12917-022-03387-1

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…Just like previous studies on the function of the pituitary, we initially expected that the reason for the high fecundity of sheep might be related to the secretion of FSH and LH hormones [8,46]. The KEGG analysis of differentially expressed DE circRNAs source genes in PF vs. MF was enriched to the cortisol synthesis and secretion (oas04927), which is also involved in the response of the pituitary to steroid and participate in the breeding process of sheep.…”

Section: Functional Enrichment Analysis Of De Circrnas Of Their Sourc...mentioning

confidence: 94%

Comparative Transcriptomics Identify Key Pituitary Circular RNAs That Participate in Sheep (Ovis aries) Reproduction

Yang,

Tang,

et al. 2023

Animals

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CircRNAs have been found to play key roles in many biological processes and have diverse biological functions. There have been studies on circRNAs in sheep pituitary, and some important circRNAs have been found. But there are still few studies on circRNAs in sheep pituitary with different fecundity. In this study, we obtained the circRNAs expression profiles in the pituitary of FecB ++ genotype Small Tail Han sheep with different fecundity and estrous phases. A total of 34,878 circRNAs were identified in 12 pituitary samples, 300 differentially expressed circRNAs (DE circRNAs) (down: 104; up: 196) were identified in polytocous sheep in the follicular phase (PF) and monotocous sheep in the follicular phase (MF) (PF vs. MF), and 347 DE circRNAs (down: 162; up: 185) were identified in polytocous sheep in the luteal phase (PL) and monotocous sheep in the luteal phase (ML) (PL vs. ML). Cortisol synthesis and secretion pathway (follicular phase) and estrogen signaling pathway (luteal phase) were obtained by functional enrichment analysis of circRNAs source genes. Competing endogenous RNA (ceRNA) network analysis of key DE circRNAs revealed that oar-circ-0022776 (source gene ITPR2, follicular phase) targeted oar-miR-432, oar-circ-0009003 (source gene ITPR1, luteal phase) and oar-circ-0003113 (source gene PLCB1, luteal phase) targeted oar-miR-370-3p. We also explored the coding ability of DE circRNAs. In conclusion, our study shows that changes in the pituitary circRNAs may be related to the response of the pituitary to steroid hormones and regulate the reproductive process of sheep by affecting the pituitary function. Results of this study provide some new information for understanding the functions of circRNAs and the fecundity of FecB ++ genotype sheep.

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Section: Functional Enrichment Analysis Of De Circrnas Of Their Sourc...mentioning

confidence: 94%

Comparative Transcriptomics Identify Key Pituitary Circular RNAs That Participate in Sheep (Ovis aries) Reproduction

Yang,

Tang,

et al. 2023

Animals

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“…More importantly, these techniques allowed researchers to explore the molecules involved in the medical conditions of pregnant females suffering from ectopic pregnancy, hypertension disorder or diabetes mellitus [ 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. Research using metabolomics was also conducted to find biomarkers in serum to foretell litter size in sheep during 7 to 70 days of gestation, or metabolites such as FSH, P4, AMH and amino acids to predict the number of ovulated oocytes following ovarian super stimulation [ 32 , 33 , 34 ]. Thus, the latest approaches empowered us to identify potential biomarkers in the very early pregnancy stages of ewes.…”

Section: Introductionmentioning

confidence: 99%

Early Pregnancy Markers in the Serum of Ewes Identified via Proteomic and Metabolomic Analyses

Zhai,

Xia,

Shi

et al. 2023

IJMS

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The diagnosis of ewes’ pregnancy status at an early stage is an efficient way to enhance the reproductive output of sheep and allow producers to optimize production and management. The techniques of proteomics and metabolomics have been widely used to detect regulatory factors in various physiological processes of animals. The aim of this study is to explore the differential metabolites and proteins in the serum of pregnant and non-pregnant ewes by proteomics and metabolomics. The serum of ewes at 21, 28 and 33 days after artificial insemination (AI) were collected. The pregnancy stratus of the ewes was finally determined through ultrasound examination and then the ewes were grouped as Pregnant (n = 21) or N on-pregnant (n = 9). First, the serum samples from pregnant or non-pregnant ewes at 21 days after AI were selected for metabolomic analysis. It was found that the level of nine metabolites were upregulated and 20 metabolites were downregulated in the pregnant animals (p < 0.05). None of these differential metabolomes are suitable as markers of pregnancy due to their small foldchange. Next, the proteomes of serum from pregnant or non-pregnant ewes were evaluated. At 21 days after AI, the presence of 321 proteins were detected, and we found that the level of three proteins were upregulated and 11 proteins were downregulated in the serum of pregnant ewes (p < 0.05). The levels of serum amyloid A (SAA), afamin (AFM), serpin family A member 6 (SERPINA6) and immunoglobulin-like domain-containing protein between pregnant and non-pregnant ewes at 21-, 28- and 33-days post-AI were also analyzed via enzyme-linked immunosorbent assay (ELISA). The levels of SAA and AFM were significantly higher in pregnant ewes than in non-pregnant ewes, and could be used as markers for early pregnancy detection. Overall, our results show that SAA and AFM are potential biomarkers to determine the early pregnancy status of ewes.

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Regulation of Milk Fat Synthesis: Key Genes and Microbial Functions

Yu,

Fu,

Jin

et al. 2024

Microorganisms

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Milk is rich in a variety of essential nutrients, including fats, proteins, and trace elements that are important for human health. In particular, milk fat has an alleviating effect on diseases such as heart disease and diabetes. Fatty acids, the basic units of milk fat, play an important role in many biological reactions in the body, including the involvement of glycerophospholipids and sphingolipids in the formation of cell membranes. However, milk fat synthesis is a complex biological process involving multiple organs and tissues, and how to improve milk fat of dairy cows has been a hot research issue in the industry. There exists a close relationship between milk fat synthesis, genes, and microbial functions, as a result of the organic integration between the different tissues of the cow’s organism and the external environment. This review paper aims (1) to highlight the synthesis and regulation of milk fat by the first and second genomes (gastrointestinal microbial genome) and (2) to discuss the effects of ruminal microorganisms and host metabolites on milk fat synthesis. Through exploring the interactions between the first and second genomes, and discovering the relationship between microbial and host metabolite in the milk fat synthesis pathway, it may become a new direction for future research on the mechanism of milk fat synthesis in dairy cows.

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Correlation analysis of serum reproductive hormones and metabolites during multiple ovulation in sheep

Cited by 3 publications

References 58 publications

Comparative Transcriptomics Identify Key Pituitary Circular RNAs That Participate in Sheep (Ovis aries) Reproduction

Comparative Transcriptomics Identify Key Pituitary Circular RNAs That Participate in Sheep (Ovis aries) Reproduction

Early Pregnancy Markers in the Serum of Ewes Identified via Proteomic and Metabolomic Analyses

Regulation of Milk Fat Synthesis: Key Genes and Microbial Functions

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