Correlation between antifungal resistance and virulence factors in Candida albicans recovered from vaginal specimens

El-Houssaini, Houdaii H.; Elnabawy, Omnia M.; Nasser, Hebatallah A.; Elkhatib, Walid F.

doi:10.1016/j.micpath.2018.12.028

Cited by 51 publications

(55 citation statements)

References 62 publications

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“…Similar results have been reported by El-Houssaini et al from Egypt 28 and Deorukhkar et al from India 35 . They revealed a significant positive correlation between biofilm formation and phospholipase production.…”

Section: Figuresupporting

confidence: 91%

“…Based on our results, the highest proteinase activity was observed in 72.85% of C. albicans. Previous studies have reported the rate of proteinase production to be 40% 28 , 82.1% 29 , and 97% 30 . Additionally, phospholipases are responsible for the hydrolysis of phospholipids, which help Candida invade the cells and destroy the host cell membrane.…”

Section: Figurementioning

confidence: 98%

“…Ramos Lde et al reported that all C. albicans isolates (100%) from cutaneous candidiasis produced both aspartyl protease and phospholipase 31 . In addition, El-Houssaini et al reported that 42% of C. albicans isolates from vaginal swab specimens produced phospholipase 28 .…”

Section: Figurementioning

confidence: 99%

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Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Mohammadi

Ghasemi

Familsatarian

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: The aim of this study was to evaluate some virulence factors in Candida albicans isolates from patients with onychomycosis and determine the correlation between these factors and the antifungal resistance profile. Methods: Seventy species of C. albicans were confirmed using polymerase chain reaction amplification of the HWP1 gene. According to the Clinical & Laboratory Standards Institute guidelines, the susceptibility profile of four antifungal agents was investigated, and the production of aspartyl protease, phospholipase, haemolysin, and biofilm was determined. The correlation between these profiles was also investigated. Results: The isolates indicated different levels of resistance and production of virulence factors. Significant correlations were observed between the minimum inhibitory concentration (MIC) of fluconazole/itraconazole and biofilm production, between phospholipase production and fluconazole/ itraconazole MIC, and between fluconazole MIC and hemolytic activity in C. albicans isolates. The results also showed significant correlations between phospholipase activity and biofilm production. Conclusions: Our findings will contribute to a better understanding of the pathogenesis of C. albicans and characterize the relationship between virulence factors and antifungal resistance, which may suggest new therapeutic strategies considering the possible involvement of the virulence mechanism in the effectiveness of treatment.

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Section: Figuresupporting

confidence: 91%

Section: Figurementioning

confidence: 98%

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Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Mohammadi

Ghasemi

Familsatarian

et al. 2020

Rev. Soc. Bras. Med. Trop.

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“…The extracellular hydrolytic enzymes including secreted aspartyl proteinase and phospholipases 7 . Furthermore, the survival and ability of Candida albicans to establish infections within humans are mainly related to its ability to produce elemental iron through haemolysin production.…”

Section: Introductionmentioning

confidence: 99%

Assessment of virulence factors and antifungal susceptibility of Candida species isolated from catheter associated urinary tract infections

Ismail

El-Haliem

Aboelmagd

2020

Al-Azhar International Medical Journal

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Background: Catheter associated urinary tract infection (CAUTI) is a crucial attribute to virulence of organisms. Emergency of non albicans Candida (NAC) species become common due to several virulence factors, which play an important role in pathogenicity and their resistance to treatment. Aim of the work: To investigate different virulence factors of Candida spp. isolated from CAUTI patients and evaluate their antifungal susceptibility. Patients and Methods: Urine samples collected from 219 indwelling catheters of ICU patients. Candida isolates identified using Germ tube test, Chrom ID agar, Corn meal tween agar, VITEK 2 system and Integral System Yeast Plus (ISYP) used to detect antifungal susceptibility. Enzymatic, haemolytic activity and biofilm formation assessed by standard methods. Results: The incidence of candiduria was 50/219 (22.8%). Candida tropicalis was predominant one (54.0%) followed by C. albicans (26.0%) and C. glabrata (20.0%). Females gender (64.0%), Diabetes (50.0%) associated with positive candiduria, while antibiotic therapy was predominant risk factor (78.0%). C. albicans have highest enzymatic activity for phospholipase (84.6 %), proteinase (84.6%) and haemolytic activity (100%), while C. tropicalis have highest biofilm formation (100%). NAC spp. showed higher rates of resistance to azole drugs (Clotrimazole 77.6%, Miconazole 70.3%, Econazole 63.2%, Nystatine 44.3%), while C. albicans didn't show any resistance. Conclusion: Candiduria is prevalent among catheterized patients with shift toward NAC spp. causing nosocomial UTI. A strong relationship between host risk factors (old age, antibiotic use, catheterization, female sex, ICU stay, diabetes) and expression of various virulence factors of Candida spp. causing candiduria and resistance to antifungal drugs.

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“…The presence of virulence genes and the increasing prevalence of resistance to antifungal therapy have contributed to the pathogenicity of Candida albicans. Nevertheless, the association between the resistance profiles and virulence genes of C. albicans has not been studied sufficiently [17], especially in invasive C. albicans infections.…”

Section: Introductionmentioning

confidence: 99%

Study of Antifungal Susceptibility, Virulence Genes and Biofilm Formation in Candida albicans

Shrief¹,

Zaki²,

El-Sehsah³

et al. 2019

TOMICROJ

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Background: Candida albicans has emerged as an important nosocomial pathogen. The morbidity and mortality associated with this pathogen are related to the presence of virulence genes and antifungal resistance. The objective of the present study was to investigate the prevalence of antifungal resistance, biofilm formation and some virulence genes such as ALS1, PLB1, INT1, SAP1 and HWP1, among clinical isolates of Candida albicans recovered from immunocompromised patients. Methods: The study included one hundred C. albicans isolates identified phenotypically and by a molecular technique using Polymerase Chain Reaction (PCR). The identified C. albicans was further subjected to antifungal study by the microdilution method, biofilm study and molecular study for virulence genes by PCR. Results: The resistance to antifungal drugs, fluconazole, caspofungin and itraconazole was 8% for each of them and for amphotericin B, it was 9%. The prevalence of the studied virulence genes was HWP1 77%, INT1 72%, ALS1 65%, SAP1 65% and PLB1 52%. The biofilm capacity was identified by the microplate method in 58% of C. albicans. The OD was intense in 20 isolates, moderate in 21 isolates and mild in 17 isolates. There was a statistically significant increase in the prevalence of the studied virulence genes INT1, ALS1, HWP1, SAP1 and PLB1 among biofilm forming C. albicans as compared to non-biofilm forming isolates (P=0.0001). Additionally, the resistance to fluconazole, itraconazole and caspofungin was statistically, significantly higher in C. albicans with the capacity to form biofilm as compared to non-biofilm forming C. albicans. Conclusion: The present study highlights the prevalence of resistance to antifungal drugs among C. albicans which are not uncommon. Moreover, there was a high prevalence of INT1, ALS1, HWP1, SAP1 and PLB1 genes in C. albicans. The resistance to antifungal drugs was common among isolates with the capacity to form the biofilm. There was an association between the biofilm formation and virulence genes.

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Correlation between antifungal resistance and virulence factors in Candida albicans recovered from vaginal specimens

Cited by 51 publications

References 62 publications

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Assessment of virulence factors and antifungal susceptibility of Candida species isolated from catheter associated urinary tract infections

Study of Antifungal Susceptibility, Virulence Genes and Biofilm Formation in Candida albicans

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