Correlation between CTLA4 genetic polymorphisms, its serum protein level and the susceptibility to recurrent spontaneous abortion

Abstract

Present study was aimed to detect the influence of cytotoxic T-lymphocyte associated protein 4 (CTLA4) gene polymorphisms for the risk of recurrent spontaneous abortion (RSA), as well as the serum level of CTLA4 protein in RSA patients.One hundred thirty-three RSA patients and 146 healthy persons were recruited in this case–control study. PCR-RFLP was used to genotype the CTLA4 gene polymorphisms both in case and control groups. Serum level of CTLA4 was detected by ELISA kit. Quantitative variables were compared by t test or Mann–Whitney U test between groups. Qualitative variables were evaluated by χ2 test or Fisher exact test. Association strength was expressed by odds ratios (ORs) and 95% confidence intervals (95% CIs).G allele of rs4553808 (P = .027, OR = 0.570, 95% CI = 0.345–0.942) and T allele of rs5742909 (P = .027, OR = 0.570, 95% CI = 0.345–0.942) were distinctly associated with reduced susceptibility of RSA. Distinctly negative association has been discovered between rs231775 AA genotype and RSA susceptibility (P = .040, OR = 0.427, 95% CI = 0.188–0.973). CTLA4 protein had significantly higher serum level in RSA patients than in healthy controls (P = .028). In RSA patients, AA genotype carriers had higher CTLA4 serum level than that GG genotype carriers (17.83 ± 6.35 ng/mL vs 10.41 ± 7.28 ng/mL, P = .039).Minor alleles of CTLA4 polymorphisms might inhibit the RSA susceptibility via upregulated the protein expression level.

The Influence of CD28 Gene Polymorphism in Transfusion Reaction after Transfusing Leukoreduced Blood Components

“…One of the reasons for spontaneous abortion was regulated by immune-related genes [29]. There were several studies indicating that spontaneous abortion was related to CTLA-4 and CD28 polymorphisms and serum level [30][31][32][33]. To protect the fetus during pregnancy, maternal alloimmunity reactions would be inhibited by the mother's own Treg in opposition to paternal antigens among embryo cells [34].…”

Otoimmün tiroid hastalıklarında CTLA-4 geninin in silico analizinin değerlendirilmesi

“…Farklı izoformları kodlayan alternatif transkripsiyonel ek varyantları karakterize edilmiştir. Membrana bağlı izoform, bir disülfür bağı ile birbirine bağlanmış bir homodimer olarak işlev görürken, çözünür izoformu monomer olarak işlev görmektedir (2)(3)(4)(5). CTLA-4; (CD152 olarak da bilinir) immünolojik bir kontrol noktasıdır ve periferik toleransın korunmasında merkezi ve önemli bir role sahiptir (6,7).…”

The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows

“…CTLA-4, a competitive antagonist for CD28-CD80/86 binding, can further impede the priming and activation of T cells, and belongs to the negative regulatory stimulation factor of T cells' functions [13]. CTLA-4 is associated with many autoimmune diseases, and some researches proved that CTLA-4 gene plays a key role in the immune tolerance of maternal-fetal interface and is related to the occurrence of abortion [14]. Galectin-1, as the galectin first discovered, is an anti-inflammatory mediator and also immunosuppressive factor.…”