Correlation between hypoosmotic swelling test and breeding soundness evaluation of adult Nelore bulls

Abstract

This study aimed at evaluating the relationship between physical and morphological semen features with the hypoosmotic swelling (HOS) test in raw semen of adult Nelore bulls classified as sound and unsound for breeding. Two hundred and six Nelore bulls aging from 3 to 10 years of age were subjected to breeding soundness examination. After physical and morphological semen examination, HOS test was done. After the breeding soundness examination, 94.2% of the bulls were classified as sound for breeding. There was no difference between the average scrotal circumference of bulls classified as sound and unsound for breeding (P>0.05), but there was difference between all semen physical and morphological aspects of bulls classified as sound and unsound for breeding (P>0.05), and no difference resulted in the mean percentage of reactive spermatozoa to HOS test results both for sound (38.4±17.9) and unsound animals (39.5±16.4; P>0.05), with no Pearson correlation between the HOS test and variables. According to these results HOS test can not be used alone to predict the reproductive potential of adult Nelore bulls.

Prevalence of Mastitis Pathogens and Antimicrobial Susceptibility of Isolates From Cattle and Buffaloes in Northwest of Pakistan

“…In the current study, 20 and 53% prevalence of clinical and subclinical mastitis was noted in cattle, whereas it was 11 and 66% in buffaloes belonging to different areas of Khyber Pakhtunkhwa. This prevalence of subclinical mastitis was quite higher than the previous reports of 35% subclinical mastitis in cattle of district Muzaffar Gharr ( 14), 30% in cattle of district Lahore (9), and 44% in Punjab (11), and our previously reported 42% subclinical mastitis in buffaloes of district D. I. Khan (10). This considerable increase in the prevalence of bovine mastitis is alarming, which might be because dairy farming is rapidly growing in Khyber Pakhtunkhwa, and it is reported that mastitis is significantly increasing with an increase in the number of dairy animals (10).…”

“…Mastitis is one of the most prevalent diseases of dairy animals all over the world (1), including Pakistan (11,14,15). In the current study, 20 and 53% prevalence of clinical and subclinical mastitis was noted in cattle, whereas it was 11 and 66% in buffaloes belonging to different areas of Khyber Pakhtunkhwa.…”

“…The prevalence of mastitis varied with different regions, the highest prevalence being noted in the cattle (81%) and buffaloes (82%) of district Peshawar. Previous studies also reported that the prevalence of mastitis varied with geography (10,11). Bacteriology of mastitic milk samples from cattle and buffaloes showed that Staphylococci spp.…”

“…A total of 633 mastitic milk samples were further processed for the identification of bacteria at the Bacteriology section of the Center of Microbiology and Biotechnology. Bacteria were identified up to the genus levels as described previously (11). Standard protocols were adopted to carry out bacteriology of milk samples according to the guidelines of the National Mastitis Council (NMC, http://www.nmconline.org/wpcontent/uploads/2016/09/Procedures-for-CollectingMilk-Samples.pdf and University of MN Laboratory for Udder Health, https://www.vdl.umn.edu/sites/vdl.umn.edu/files/samplecollection-guide-withpictures.pdf).…”

An Evaluation of Semen Quality of Donor Bulls in the Central Artificial Insemination Station (CAIS), Kundasale, Sri Lanka

“…The maximum sperm swelling was found as an indication of high fertility during the same study. However, Martins et al, (2011) found that even though there were significant differences in physical and morphological aspects of semen between highly fertile and poorly fertile bulls, no difference was found in the mean percentage of reactive sperm in HOS test.…”