Correlation between lipid deposition, immune-inflammatory cell content and MHC class II expression in diffuse intimal thickening of the human aorta

Quantitative analysis of the expression of caspase 3 and caspase 9 in different types of atherosclerotic lesions in the human aorta

“…Earlier we reported that the number of resident and inflammatory cells as well as proliferative activity is dramatically increased in fatty streaks, followed by the significant fall of these parameters in fibrous plaques (Bobryshev et al, 2011;Orekhov et al, 1998Orekhov et al, , 2010. The fall in both CASP3 and CASP9 expressions in advanced atherosclerotic plaques may be associated with continued domination of necrotic processes, especially in zones, adjusted to the lipid/necrotic core.…”

“…The unaffected regions of the aortas and those with atherosclerotic lesions were identified macroscopically according to the classification of the Atherosclerosis Council of the American Heart Association (Stary et al, 1994(Stary et al, , 1995, utilizing the corresponding histological evaluations as used previously (Bobryshev et al, 2011;Orekhov et al, 2010). Areas, unaffected by atherosclerosis, were defined as tissue samples with smooth luminal surfaces.…”

“…For further ultrastructural analysis, several small samples of intimal tissue (approximately 1 mm 3 , each) from different types of atherosclerotic lesions were dissected, were first fixed in 2% glutaraldehyde in PBS, then post-fixed in 1% OsO 4 , and eventually were routinely embedded into Araldite blocks, as used previously (Bobryshev et al, 2011;Orekhov et al, 2010). Ultrathin sections were stained with uranyl acetate and citrate lead, and were examined using a Hitachi H7000 electron microscope.…”

Mutation C3256T of Mitochondrial Genome in White Blood Cells: Novel Genetic Marker of Atherosclerosis and Coronary Heart Disease

“…Blood-derived cells, especially monocyte-macrophages, play an important role in atherogenesis [3], [14]–[17]. While migrating to the subendothelial space of elastic and muscular-elastic arteries, they are actively involved in the processes of inflammation and the formation of atherosclerotic lesions [3], [14]–[17].…”

“…While migrating to the subendothelial space of elastic and muscular-elastic arteries, they are actively involved in the processes of inflammation and the formation of atherosclerotic lesions [3], [14]–[17]. We can assume that the higher the level of heteroplasmy of mitochondrial DNA, the higher the probability of inhibiting the functional activity of monocytes.…”