Correlation Between Melanoma Angiogenesis and the Mesenchymal Stem Cells and Endothelial Progenitor Cells Derived from Bone Marrow

Sun, Baocun; Ni, Chunsheng; Zhang, Danfang; Liu, Yanxue; Zhang, Wenzhi; Zhao, Xiulan; Chun-hua, Zhao; Shi, Mingxia

doi:10.1089/scd.2005.14.292

Cited by 71 publications

(45 citation statements)

References 43 publications

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“…Furthermore, a role of MSC in neoangiogenesis is discussed, as the administration of MSC stimulated revascularisation of ischaemic tissues (Chen et al, 2003;Nagaya et al, 2004). A correlation between angiogenesis in melanoma and MSC has recently been demonstrated (Sun et al, 2005), and MSC have been found to transmigrate over the endothelial barrier (Schmidt et al, 2006b). In all cases, MSCs had to cover a distance to reach the target area.…”

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confidence: 99%

VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma

et al. 2008

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Little is known about the factors that enable the mobilisation of human mesenchymal stem cells (MSC) from the bone marrow into the blood stream and their recruitment to and retention in the tumour. We found specific migration of MSC towards growth factors present in pancreatic tumours, such as PDGF, EGF, VEGF and specific inhibitors Glivec, Erbitux and Avastin interfered with migration. Within a few hours, MSC migrated into spheroids consisting of pancreatic cancer cells, fibroblasts and endothelial cells as measured by time-lapse microscopy. Supernatant from subconfluent MSC increased sprouting of HUVEC due to VEGF production by MSC itself as demonstrated by RT-PCR and ELISA. Only few MSCs were differentiated into endothelial cells in vitro, whereas in vivo differentiation was not observed. Lentiviral GFP-marked MSCs, injected in nude mice xenografted with orthotopic pancreatic tumours, preferentially migrated into the tumours as observed by FACS analysis of green fluorescent cells. By immunofluorescence and intravital microscopic studies, we found the interaction of MSC with the endothelium of blood vessels. Mesenchymal stem cells supported tumour angiogenesis in vivo, that is CD31 þ vessel density was increased after the transfer of MSC compared with siVEGF-MSC. Our data demonstrate the migration of MSC toward tumour vessels and suggest a supportive role in angiogenesis.

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confidence: 99%

VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma

et al. 2008

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“…The findings showed that BMS cells play a key role in tumor neovascularization and glioma progression. Evidence also indicates that grafted MSCs directly support the tumor vasculature and growth of tumors (28,39).…”

Section: █ Discussionmentioning

confidence: 99%

Effects of the two types of human cells on outgrowth of human glioma in rats

Abdi

Eskandary²,

Nematollahi-Mahani³

2016

Turkish Neurosurgery

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In early cell-therapy studies (in the treatment of cancer), Aboody et al. (2) showed significant migration of neural stem cells (NSCs) towards the tumor area. In line with this, types of stem cells, and especially mesenchymal stem cells (MSCs), that have been evaluated in several tumor models including breast, prostate, and ovarian cancer are used (11,15,23). However, the data are not adequate and some are contradictory to each other.Olfactory ensheathing cell (OEC) is a neural stem cell type of the glial family like astrocytes and Schwann cells (35). In the recent decade, several reports have provided evidence that █ INTRODuCTION G lioblastoma (GBM) is one of the primary malignant brain tumors that occur most frequently. Nearly, all patients with GBM die within 12-15 months of the initial diagnosis (3,7,25). Therapies are still limited due to the ability of the blood brain barrier (BBB) to prevent the crossing of a broad range of anti-cancer agents (10,19). Therefore, the cure of GBM is a major concern. Accordingly, there is a lot of interest in devising novel approaches, such as stem cell therapy, to treat patients with GBM (10,12).AIm: Glioblastoma multiforme (GBM) is one of the malignant brain tumors that occur most frequently. Despite advances in therapy techniques, the cure of GBM is a major concern. Accordingly, there is a lot of interest in devising novel approaches, such as stem cell therapy, to treat patients with GBM. The aim of this study was to investigate the effects of human bone marrow stem (BMS) cells as well as human olfactory ensheathing cells (OECs) on the outgrowth of U87 glioma in rats. mATERIAl and mEThODS:OECs and BMS cells were obtained from volunteers. After verification of the stem cell type by flow cytometry and immunocytochemistry (ICC), cells were labeled and injected into human glioma-bearing rats. Magnetic resonance imaging (MRI), Hematoxylin and Eosin (H&E), and Immunohistochemistry (IHC) were utilized to assess the properties of the groups. RESulTS:We found extensive migration and homing of the OECs and BMS cells towards the tumor area. H&E and IHC staining indicated that the grafted OECs survived and prevented the development of glioma. BMS cells supported proliferation and new vessel formation, and metastasis in glioma tissue. CONCluSION:OECs and BMS cells can pass the blood brain barrier and reach the glioma mass. Therefore, this approach can be a potentially powerful method for the delivery of therapeutic agents to malignant brain tumors. In addition, these cells may be genetically modified in order to specifically express tumor-suppressive factors.

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“…[35][36][37][38] Their presence in circulation is also used as a surrogate marker for a variety of pathologies, including heart disease and cancer. [39][40][41] However, their widespread use for diverse applications and experimental investigations is often limited by the low abundance of this population. Ex vivo expansion of EPCs led to the identification of two distinct fractions of EPCs based on their proliferation state.…”

Section: Discussionmentioning

confidence: 99%

Immortalized Functional Endothelial Progenitor Cell Lines from Umbilical Cord Blood for Vascular Tissue Engineering

Sobhan

Seervi²,

Joseph³

et al. 2012

Tissue Engineering Part C: Methods

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Endothelial progenitor cells (EPCs) play a significant role in multiple biological processes such as vascular homeostasis, regeneration, and tumor angiogenesis. This makes them a promising cell of choice for studying a variety of biological processes, toxicity assays, biomaterial-cell interaction studies, as well as in tissueengineering applications. In this study, we report the generation of two clones of SV40-immortalized EPCs from umbilical cord blood. These cells retained most of the functional features of mature endothelial cells and showed no indication of senescence after repeated culture for more than 240 days. Extensive functional characterization of the immortalized cells by western blot, flow cytometry, and immunofluorescence studies substantiated that these cells retained their ability to synthesize nitric oxide, von Willebrand factor, P-Selectin etc. These cells achieved unlimited proliferation potential subsequent to inactivation of the cyclin-dependent kinase inhibitor p21, but failed to form colonies on soft agar. We also show their enhanced growth and survival on vascular biomaterials compared to parental cultures in late population doubling. These immortalized EPCs can be used as a cellular model system for studying the biology of these cells, gene manipulation experiments, cell-biomaterial interactions, as well as a variety of tissue-engineering applications.

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Correlation Between Melanoma Angiogenesis and the Mesenchymal Stem Cells and Endothelial Progenitor Cells Derived from Bone Marrow

Cited by 71 publications

References 43 publications

VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma

VEGF expression by mesenchymal stem cells contributes to angiogenesis in pancreatic carcinoma

Effects of the two types of human cells on outgrowth of human glioma in rats

Immortalized Functional Endothelial Progenitor Cell Lines from Umbilical Cord Blood for Vascular Tissue Engineering

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