Correlation between metallothionein and energy metabolism in sea bass, Dicentrarchus labrax, exposed to cadmium

Cattani, Otello; Serra, R.; Isani, Gloria; Raggi, Giampaolo; Cortesi, P.; Carpenè, Emilio

doi:10.1016/0742-8413(95)02087-x

Cited by 35 publications

(33 citation statements)

References 34 publications

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“…Some studies documented the accumulation of cadmium and copper in the gills, kidneys and liver and the pathological changes of varying severity in the above mentioned organs in sea bass. (Cattani et al, 1996;Giari et al, 2007;Romeo et al, 2000;Viarengo et al, 1997). In this work, we show preliminarily that the cultures of isolated blood leukocytes of D. labrax were able to incorporate Cd and Cu after treatment, thus confirming that these metals could cross membranes (for a review see Foulkes (2000)), and accumulate in the cells.…”

Section: Discussionmentioning

confidence: 60%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c tThe immunotoxic effects of heavy metals on blood leukocytes of sea bass (Dicentrarchus labrax) were examined. The cells, separated by a discontinuous Percoll-gradients, were exposed in vitro to various sublethal concentrations of cadmium and copper (10 À 7 M, 10 À 5 M, and 10 À 3 M) and their immunotoxic effect was then evaluated by measuring neutral red uptake, MTT assay, DNA fragmentation and Hsp70 gene expression. First of all, we demonstrated that the cells treated in vitro could incorporate Cd and Cu. A relationship between heavy metal exposure and dose-time-dependent alterations in responses of leukocytes from blood was found for both metals, but copper was more immunotoxic than cadmium in all assays performed. A significant reduction in the cells' ability to uptake neutral red and viability by MTT assay was recorded, indicating that both cadmium and copper could change the membrane permeability, inducing cellular apoptosis when the concentration of metals reached 10 À 3 M. The apoptotic effect may also explain the high level of cytotoxicity found when the leukocytes were exposed to higher concentration of metals. These results demonstrated that toxic effect of copper and cadmium affect on the mechanisms of cellmediated immunity reducing the immune defences of the organism.

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Section: Discussionmentioning

confidence: 60%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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“…Bebianno and Machado (1997) have proposed that measurement of metallothioneins concentrations in biomonitors can provide an accurate indication of sublethal environment increases in metal contamination. Moreover, metallothioneins may be useful in predicting metal toxicity in aquatic organisms because cellular toxicity may result after metal binding capacity of metallothioneins is exceeded and associated with the stress response in aquatic organisms (Cattani et al 1996;Tort et al 1996).…”

Section: Discussionmentioning

confidence: 99%

The effect of in situ cadmium contamination on some biomarkers in Cerastoderma glaucum

et al. 2007

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The present study was designed to detect in situ effects of cadmium on marine organisms Cerastoderma glaucum from the gulf of Gabès (Tunisia). Six sampling stations were chosen: one site, relatively far from known local source of pollution, was considered as uncontaminated site and five sites clearly exposed to anthropogenic impact. Metallothionein like protein (MTLP) and sub-cellular metal partitioning were measured in cockles (C. glaucum) gills and digestive gland. Various biomarkers were also measured, including malondialdehyde (MDA) in the digestive gland and acetylcholinesterase activity (AChE) in the remainder. The health status of the cockles was assessed by using the condition index (CI). Significant differences between sites were noted for Cd concentrations, (CI) and also for the three studied biomarkers. Significant higher biomarkers response was measured in cockles from stations located in the northern part of the gulf, which are exposed directly to industrial and urban effluents, whereas the response of most biomarkers was minimal at the reference station. Positive and significant correlations were observed between MTLP and Cd concentrations in the digestive gland and in the gills. However, it must be noted that these correlations were more significant in the digestive gland, suggesting that compared to the gills, the digestive gland of C. glaucum is more suitable for monitoring metal pollution. The subcellular distribution of Cd showed that the soluble fraction was the major compartment for Cd storage, a pattern which is due to the role of MTLP in Cd detoxication. But at the most contaminated site (EH), cadmium in the digestive gland was preferentially accumulated in the insoluble fraction (P1) suggesting that the MTLP capacity in binding metals was not sufficient to avoid the binding of Cd to the insoluble fraction. Furthermore, the MTLP concentrations in the cockles from this site are lower than expected. So in highly polluted sites, MTLP in C. glaucum should not be used as a useful biomarker for metal pollution. The lipid peroxidation as presented by malondialdehyde levels, and MT-like protein concentrations increased in cockles exposed to cadmium contamination. We can, therefore, hypothesize that Cd could induce MTLP synthesis and MDA increase. While AChE had distinct and specific pattern showing that cadmium is not the only factor of the inhibition of cholinesterase activity. There are other polluting inputs engendering this inhibition.

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“…data). Depletion of body glycogen and/or lipid reserves was also observed in fishes (sea bass Dicentrarchus labrax and rainbow trout Oncorhynchus mykiss), gastropods (dogwhelk Nucella lapillus) and crustaceans (crabs Scylla serrata and the harpacticoid Tigriopus japonicus) exposed to Cd, Cu and/or tributyltin (TBT) (Reddy & Bhagyalakshmi 1994, Cattani et al 1996, Clearwater et al 2002, Kwok & Leung 2005. At high concentrations of Cu and TBT, T. japonicus entered a metabolically dormant state enhancing their survival (Kwok & Leung 2005), showing that an ability to channel energy into coping with toxins (even at the expense of other biological functions) may be essential in survival of metal exposures.…”

Section: Energy Demandmentioning

confidence: 99%

Interactive effects of metal pollution and temperature on metabolism in aquatic ectotherms: implications of global climate change

Sokolova¹,

Lannig²

2008

Clim. Res.

466

270

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In light of the current rising mean temperatures and fluctuations in temperature extremes involved in global climate change, a cause-and-effect understanding of the temperaturedependent impacts of additional environmental stressors on marine life is crucial for the elaboration of how marine ecosystems will shape in the future. The thermal environment plays a direct role in the distribution and survival of marine ectotherms through the temperature-dependent effects on their physiology, as well as indirectly by affecting the organism's susceptibility to other biotic and abiotic stressors. We review studies that have investigated the effects of temperature and anthropogenic pollution by trace metals, with a focus on metabolic regulation as a major mechanism that underlies the interactive effects of temperature and metals on the physiology and survival of ectotherms. These studies suggest that impairment of energy metabolism plays a key role in the synergistic effects of these stressors, and we elaborate a mechanistic framework for understanding these interactions. Furthermore, the present study seeks to provide an impetus for future investigations in order to define more precisely the physiological mechanisms and functional properties of temperature-pollution interactions.

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Correlation between metallothionein and energy metabolism in sea bass, Dicentrarchus labrax, exposed to cadmium

Cited by 35 publications

References 34 publications

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

The effect of in situ cadmium contamination on some biomarkers in Cerastoderma glaucum

Interactive effects of metal pollution and temperature on metabolism in aquatic ectotherms: implications of global climate change

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