Correlation between sestrin2 expression and airway remodeling in COPD

Abstract

Background Airway remodeling is a major pathological characteristic of chronic obstructive pulmonary disease (COPD), and has been shown to be associated with oxidative stress. Sestrin2 has recently drawn attention as an important antioxidant protein. However, the underlying correlation between sestrin2 and airway remodeling in COPD has yet to be clarified. Methods A total of 124 subjects were enrolled in this study, including 62 control subjects and 62 COPD patients. The pathological changes in airway tissues were assessed by different staining methods. The expression of sestrin2 and matrix metalloproteinase 9 (MMP9) in airway tissues was monitored by immunohistochemistry. Enzyme-linked immunosorbent assays (ELISAs) were used to detect the serum concentrations of sestrin2 and MMP9. The airway parameters on computed tomography (CT) from all participants were measured for evaluating airway remodeling. The relationship between serum sestrin2 and MMP9 concentration and airway parameters in chest CT was also analyzed. Results In patients with COPD, staining of airway structures showed distinct pathological changes of remodeling, including cilia cluttered, subepithelial fibrosis, and reticular basement membrane (Rbm) fragmentation. Compared with control subjects, the expression of sestrin2 and MMP9 was significantly increased in both human airway tissues and serum. Typical imaging characteristics of airway remodeling and increased airway parameters were also found by chest CT. Additionally, serum sestrin2 concentration was positively correlated with serum MMP9 concentration and airway parameters in chest CT. Conclusion Increased expression of sestrin2 is related to airway remodeling in COPD. We demonstrated for the first time that sestrin2 may be a novel biomarker for airway remodeling in patients with COPD.

Effect of doxofylline on pulmonary inflammatory response and oxidative stress during mechanical ventilation in rats with COPD

“…Cigarette smoke exposure offers the closest environment to the disease in humans, and this method has been successfully adopted in the construction of various animal COPD models [ 12 ]. In the evaluation of animal COPD models, pulmonary histopathological examination can determine whether the pathological changes in the small airways and pulmonary parenchyma are consistent with the typical changes seen in COPD [ 13 ]. In the present experiment, the COPD rat model was constructed by fumigation and intratracheal injection of LPS.…”

Target Sestrin2 to Rescue the Damaged Organ: Mechanistic Insight into Its Function

“…It was reported that both serum level and tissue expression of Sestrin2 are higher in patients with COPD. Furthermore, a positive correlation was found between serum Sestrin2 level and serum MMP9 level or airway remodeling in chest CT-scan [191]. Similarly, it was uncovered that patients with asthma have higher plasma levels of Sestrin2.…”

Serum Sestrin2 Was Lower in Septic Shock Patients with Cardiomyopathy

“…Experienced nurses took blood samples from septic shock patients within 24 hours of admission and from healthy controls during a physical examination. Blood samples were obtained from research participants in procoagulant tubes, and the supernatant was centrifuged after 20 minutes at 1000 rpm as the sample for testing [ 15 ]. Serum samples were kept at a temperature of minus 80 degrees Celsius (blood collection to serum storage time not exceeding 45 minutes, which can be stored for 2 months for backup) for later use.…”