Correlation between the occurrence of plasmid pUCS202 and lipopolysaccharide alterations in<i>Rhizobium</i>

Russa, Ryszard; Urbanik, Teresa; Kowalczuk, E.; Lorkiewicz, Z

doi:10.1111/j.1574-6968.1982.tb08248.x

Cited by 19 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The loss of the plasmid encoding nodulation functions in R. trifolii results in a non-nodulating phenotype and the loss of D-quinovosamine from the LPS (29). When the plasmid is regained, the cells are then capable of nodulation and D-quinovosamine can again be detected in the LPS (29). Agrohaciteriu,n.…”

Section: Resultsmentioning

confidence: 99%

Bacteriophage that can distinguish between wild-type Rhizobium japonicum and a non-nodulating mutant

Stacey¹,

Pocratsky²,

Puvanesarajah³

1984

Appl Environ Microbiol

View full text Add to dashboard Cite

A bacteriophage (phage TNI) that lyses Rhizobium japonicum 31lbl 10 was isolated from Tennessee soil. Structurally, this phage resembles the Escherichia coli phage T4, having an icosahedral head (47 by 60 nm) and a contractile tail (17 by 80 nm). An interesting feature of this phage is that it lyses all of the symbiotic defective mutants derived from R. japonicum 3Ilb1 10 that were tested, except one, mutant strain HS123. Mutant strain HS123 is a non-nodulating mutant that is defective in attachment to soybean roots. Since Rhizobium attachment to host roots is thought to be mediated by a specific cell surface interaction, it is likely that mutant strain HS123 is defective in some way in its cell surface. Mutant strain HS123 bound soybean lectin to the same extent as the wild type as measured by the binding of tritium-labeled lectin. Phage TN1 did not attach to the surface of strain HS123, nor did cells of strain HS123 inactivate phage TN1. A hot phenol-water cell extract from the wild-type inactivated phage TN1, whereas a similar cell extract from mutant HS123 did not. Capsular polysaccharide isolated from mutant or wild type did not inactivate the phage. Capsular polysaccharide and exopolysaccharide from the mutant and wild type do not differ in sugar composition. These results indicate that capsular polysaccharide may not play a role in attachment to the plant root surface and that other cell wall components may be important.

show abstract

Section: Resultsmentioning

confidence: 99%