Correlation between the order parameter and the steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene and an evaluation of membrane fluidity

Pottel, Hans; Meer, W. van der; Herreman, W.

doi:10.1016/0005-2736(83)90331-0

Cited by 178 publications

(86 citation statements)

References 27 publications

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“…This probe remains on the surface of living endothelial cells for up to 4 h (31). The second probe, DPH, is used to measure the average molecular packing order of the deeper lipid or hydrophobic regions of membranes throughout the cell (9)(10)(11).…”

Section: Discussionmentioning

confidence: 99%

“…The purpose of this study was to provide further evidence in support of the interactions of the 21-aminosteroids with lipid or hydrophobic domains of cells, the presumed site of action for these agents. Brain microvessel endothelial cells (BMECs) were labeled with well-known fluorophores, 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethyammoniumphenyl)-6-diphenyl-1,3,5-hexatriene, ptoluenesulfonate (TMA-DPH), and fluorescence anisotropy and lifetime measures of these probes used to examine the interactions of 21-aminosteroids with cellular lipid domains (7)(8)(9)(10)(11). BMECs represented an appropriate experimental system since the microvasculature of the central nervous system has been suggested as a major target for oxygen radicals in trauma and stroke (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

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21-aminosteroid and 2-(aminomethyl)chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers

Shi

Cavitt

Audus

1995

Free Radical Biology and Medicine

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Abstract:Studies were conducted to demonstrate 21-aminosteroid distribution into the hydrophobic or lipid domains of biological membranes, a presumed site at which these compounds inhibit lipid peroxidation. Bovine brain microvessel endothelial cells (BMECs) were labeled with diphenylhexatriene fluorophores and interactions with cell membranes characterized with fluorescence anisotropy and lifetimes. Two 21-aminosteroids (U-74500A and U74006F) were shown to preferentially alter the fluorescence anisotropy and lifetime parameters of the diphenylhexatriene probe distributing into membranes throughout the BMECs. Little or no effect of the compounds on the fluorescence parameters of the diphenylhexatriene probe the localized on the surface of BMEC plasma membranes. By contrast, cholesterol used as a positive control altered substantially the fluorescence parameters of BMECs labeled with either diphenylhexatriene probe. Results suggest 21-aminosteroid-induced changes in the molecular packing order and drug:fluorescent probe interactions in membrane hydrophobic (or lipid) domains throughout the BMEC. Concentrations of 21-aminosteroids altering the fluorescence parameters of diphenylhexatriene labeled BMECs correspond to those concentrations of 21-aminosteroids effective in vitro in inhibition lipid peroxidation. Evidence for 21-aminosteroid association with the hydrophobic domains in brain microvessel endothelial cells. Free Rad. Biol. Med. 11, 361-371. PMID: 1797623. Publisher's official version: http://dx

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

21-aminosteroid and 2-(aminomethyl)chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers

Shi

Cavitt

Audus

1995

Free Radical Biology and Medicine

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“…Lipid order parameter, S, was calculated from the anisotropy by the following analytical expression [16]:…”

Section: Fluorescence Anisotropy Measurementsmentioning

confidence: 99%

“…The theoretical value of r 0 is 0.4, while experimental values of r 0 lie between 0.362 and 0.394 [16]. was calculated from the EPR spectra using Eq.…”

Section: Fluorescence Anisotropy Measurementsmentioning

confidence: 99%

Structural characterization of liposomes made of diether archaeal lipids and dipalmitoyl-L-α-phosphatidylcholine

Gmajner¹,

Grabnar²,

Žnidarič³

et al. 2011

Biophysical Chemistry

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D

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“…(4) of a reduced m~mber of membrane lipid fatty acid chains and can be related to the order intramembranous particles in PMN from PCD patients. parameter S, if certain precautions are taken (27,28). Lipid…”

Section: Discussionmentioning

confidence: 99%

Membrane Fluidity of Polymorphonuclear Leukocytes from Children with Primary Ciliary Dyskinesia

Kantar

Giorgi

et al. 1993

Pediatr Res

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ABSTRACT. Plasma membrane fluidity and heterogeneity of polymorphonuclear leukocytes (PMN) were investigated in seven children with primary ciliary dyskinesia (PCD) and 17 healthy controls. Membrane fluidity and heterogeneity were studied by measuring the steady state fluorescence anisotropy and fluorescence decay of 1-(Ctrimethylammoniumphenyl)d-phenyl-1,3 J-hexatriene (TMA-DPH) incorporated into PMN plasma membrane. Our results show an increase in membrane fluidity at the surface level of PMN from patients with PCD. Distribution analysis of TMA-DPH lifetime values indicate an increase in membrane heterogeneity in subjects with PCD. The observed changes in the physicochemical properties of the membrane could lead to alterations in the function of PMN from children with PCD. (Pediarr Res 34: 725-728, 1993) Abbreviations PMN, polymorphonuclear leukocyte PCD, primary ciliary dyskinesia TMA-DPH, 144-trimethy1ammoniumphenyl)-6-phenyl-1,3,5-hexatriene FMLP, N-formyl-methionyl-leucyl-phenylalanine r,, steady state fluorescence anisotropy PMA, phorbol myristate acetate PMN from subjects with PCD can have an abnormal locomotory system. Defects in orientation (l,2), migration (1-3) and chemotaxis (3-5) have been reported. All these activities are the result of complex events mediated by the plasma membrane and cytoskeleton of PMN. Specific and dynamic interactions of plasma membrane components are essential for an adequate membrane function (6). Membrane fluidity has an important role in modulating cell functions, affecting the conformation of membrane proteins and the exposure and diffusion of membrane components. Fluidity is a complex physicochemical feature that depends upon mobility and order of membrane constituents (6, 7). Changes in composition and molecular organization are the principal determinants of alterations of membrane fluidity observed in many human diseases (8,9).In this study, we have evaluated plasma membrane fluidity of PMN obtained from children with PCD by measuring r, and fluorescence decay of a probe incorporated into the plasma membranes. As a fluorescent probe, we have used TMA-DPH, which is incorporated at the lipid-water interface region bilayer.

show abstract

Correlation between the order parameter and the steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene and an evaluation of membrane fluidity

Cited by 178 publications

References 27 publications

21-aminosteroid and 2-(aminomethyl)chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers

21-aminosteroid and 2-(aminomethyl)chromans inhibition of arachidonic acid-induced lipid peroxidation and permeability enhancement in bovine brain microvessel endothelial cell monolayers

Structural characterization of liposomes made of diether archaeal lipids and dipalmitoyl-L-α-phosphatidylcholine

Membrane Fluidity of Polymorphonuclear Leukocytes from Children with Primary Ciliary Dyskinesia

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