Correlation between total hypha length and haustoria number of Pseudoidium neolycopersici in type I trichome cells of tomato leaves

Suzuki, Tomoko; Nakao, Yoshinori; Ura, Resti; Nakaba, M.; Nishiyama, T.; Takikawa, Yuichi; Matsuda, Yoshinori; Kakutani, Koji; Bai, Yuling; Nonomura, Teruo

doi:10.1007/s13313-020-00718-4

Cited by 2 publications

(4 citation statements)

References 48 publications

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“…Using the KH-2700 DM, we could observe functional mature haustoria (haustoria beneath primary appressoria and haustoria beneath hyphal appressoria) in single P. neolycopersiciinfected trichome cells. With a field-emission scanning electron microscope (FE-SEM), we further confirmed that the functional mature haustorium contains a haustorial body and several haustorial lobes embedded in an extrahaustorial matrix and surrounded by an extrahaustorial membrane [38].…”

Section: Discussionmentioning

confidence: 53%

“…Moneymaker (MM) include a length of 1.5-2.5 mm on a multicellular base with a small glandular tip; they are more abundant and larger than other types of trichomes. In our previous studies, we succeeded in observing mature haustoria in P. neolycopersici-invaded trichomes using a high-fidelity digital microscopy (DM) without chemical treatment [37] and clarified the morphological characteristics of functional P. neolycopersici haustoria in type I trichomes of MM using field-emission scanning electron microscopy [38]. Our results showed a positive correlation between the number of haustoria per hypha and the hyphal lengths on trichomes.…”

Section: Introductionmentioning

confidence: 76%

“…Twenty-five leaf segments (approximately 1 × 1 cm in area) were collected from KTP-03-inoculated MM seedlings. All hyphae (except haustoria in trichome cells) that grew on the trichome cells were successfully removed using a small glass needle installed in a micromanipulator [38]. Haustoria in type I trichomes were directly observed under the KH-2700 DM and a BX-60 light and epifluorescence microscope (BX-60 EM; Olympus, Tokyo, Japan) without chemical treatment.…”

Section: Microscopic Observation Of P Neolycopersici Haustoria Formed...mentioning

confidence: 99%

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Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR

et al. 2022

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Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths of the plant epidermis, are suitable for monitoring infection processes of powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On the trichomes, tomato powdery mildew (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced a maximum of four vigorously elongated hyphae per conidium, which stopped growth approximately 12 days after inoculation. Single trichome cells, invaded by fungal hyphae at various fungal infection stages during the 12-day period after the inoculation of single conidia, were cut at the bases and directly collected with small precision scissors (i.e., microscissors) held by the manipulator under a DM. Subsequently, suc-polymerase chain reaction (PCR) (reverse transcription (RT)-PCR followed by nested (N)-PCR) was conducted to explore gene expression in the infected trichome. We selected intron-containing genes from tomatoes and powdery mildew fungi for the detection of constitutive gene transcripts, namely plasma membrane H+-ATPase (LHA2) and β-tubulin 2 (TUB2) genes. In suc-PCR, a single band from spliced mRNAs of both LHA2 and TUB2 genes were detected, suggesting that both genes were successfully transcribed in single KTP-03-infected trichomes. With combined primers for both LHA2 and TUB2 (multiplex RT-PCR/N-PCR), two bands were detected through the amplification of intron-spliced mRNAs of both genes. Therefore, our single-trichome cell PCR amplification method is effective for detecting the expression patterns of genes from both tomato and powdery mildew fungus. Combinations of digital microscopy, microscissors, and multiplex RT-PCR/N-PCR amplification techniques will be useful for simultaneously analysing the molecular interactions between plants and powdery mildew fungi at the level of single tomato leaf trichome cells. Also, this employed technique will be of benefit in other plant species and crops, possessing leaf trichome cells, to elucidate the molecular interactions between plants and pathogens.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 76%

Section: Microscopic Observation Of P Neolycopersici Haustoria Formed...mentioning

confidence: 99%

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Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR

et al. 2022

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“…No decorrer de seu desenvolvimento intracelular, os pegs de penetração deram origem aos haustórios. Em alguns estudos, observou-se a germinação de conídios e o sucesso da infecção por Pseudoidium neolycopersici em tricomas glandulares do tomateiro (SUZUKI et al 2020, NÉMETH et al 2021) por meio do peg de penetração oriundo de apressórios dos tubos germinativos (SUZUKI et al 2018).…”

Section: Tricomas Como Locais De Infecção Por Fun-gos E Oomicetosunclassified

Tricomas: defesa ou vulnerabilidade para a planta na interação com microrganismos?

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Dallagnol

2022

Revisão Anual De Patologia De Plantas

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The plant surface is one of the first sites of contact with microorganisms, where structures such as the cuticle and trichomes are responsible for interfering with this initial interaction. Trichomes, while protecting plants from microorganisms, can also facilitate infection by pathogens. The density of trichomes varies among species, and the amount and type of these structures in plant organs can confer resistance or susceptibility of plants to microorganisms. The trichomes, non-glandular and glandular, act as a barrier, preventing the adherence of the infective structures of the pathogens on the plant surface. In addition, glandular trichomes also secrete exudates (products of plant metabolism), which have antimicrobial properties, negatively interfering with the development of many bacterial and fungal diseases. However, both trichomes are favorable sites for the penetration and multiplication of plant pathogens, possibly because they provide a certain level of moisture and nutrients for the development of fungi and bacteria. Contrary to the view that trichomes only provide protection against plant pathogens, by hindering their adhesion, they can also become preferential sites for infection.

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Correlation between total hypha length and haustoria number of Pseudoidium neolycopersici in type I trichome cells of tomato leaves

Cited by 2 publications

References 48 publications

Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR

Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR

Tricomas: defesa ou vulnerabilidade para a planta na interação com microrganismos?

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