Correlation between virulence and colony morphology in Vibrio vulnificus

Simpson, Linda M.; White, V K; Zane, Sandra F.; Oliver, James D.

doi:10.1128/iai.55.1.269-272.1987

Cited by 191 publications

(112 citation statements)

References 24 publications

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“…Colony morphology was studied according to Simpson et al (1987). Cultures were grown in brain heart infusion (BHI) at 37°C for 24 h and streaked on BHI agar and nutrient agar.…”

Section: Methodsmentioning

confidence: 99%

“…The capsule has the property of being resistant to serum and the phagocytic activity of polymorphonuclear cells, raising mice lethality (Yoshida et al 1985). Simpson et al (1987) found that, of 38 isolates of V. vulnificus, all virulent strains exhibited both opaque and translucent colonies when they were plated onto a variety of solid media. Using a mouse model, they found a 100% correlation between virulence and colony morphology, with the cultures composed solely of translucent colonies being avirulent.…”

Section: Table 4 Number (%) Of Virulent and Avirulent Vibrio Vulnificmentioning

confidence: 99%

“…1986) ; ability to adhere to the surface of epithelial cells (Reyes et al 1985) ; presence of pili (Gander and LaRocco 1989) and ability to utilize transferrin-bound iron (Wright et al 1990). So far, however, the most critical element of V. vulnificus virulence, according to Simpson et al (1987), is encapsulation.…”

Section: Introductionmentioning

confidence: 99%

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Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Moreno

Landgraf

1998

J Appl Microbiol

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M . L . G A RC IA M OR EN O AN D M . LA ND G RA F. 1998. The virulence factors of Vibrio vulnificusare not yet well understood. So far, many hydrolytic enzymes have been implicated in the pathogenesis of this micro-organism. The present research was carried out in order to study the presence of some of these enzymes in 133 V. vulnificus strains isolated from 45 seafood samples. The results showed that 100% of these strains were positive for the production of lecithinase and lipase (Tween-80), 99·2% for caseinolytic protease, 96·9% for DNase, 65·4% for mucinase and 46·6% for elastase. None of the strains was positive for the production of collagenase and 96% were haemolytic against sheep blood cells. In relation to colony morphology on brain heart infusion (BHI) agar and nutrient agar, 59·4% of strains showed opaque morphology on BHI agar and 57·9% on nutrient agar, 10·5% presented translucent morphology on both agars and 30·1 and 31·6% of strains showed a mixture of opaque and translucent morphology on BHI agar and nutrient agar, respectively. None of the translucent colonies was virulent to mice. Therefore, opacity was a useful marker for potential virulence. Of 45 food samples contaminated with V. vulnificus, 29 (64·4%) presented strains lethal to adult mice.

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“…Colony morphology was studied according to Simpson et al (1987). Cultures were grown in brain heart infusion (BHI) at 37°C for 24 h and streaked on BHI agar and nutrient agar.…”

Section: Methodsmentioning

confidence: 99%

Section: Table 4 Number (%) Of Virulent and Avirulent Vibrio Vulnificmentioning

confidence: 99%

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Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Moreno

Landgraf

1998

J Appl Microbiol

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“…Changing colony morphology seems to be typical for many flavobacterial species (Anderson and Ordal 1961;Högfors-Rönnholm and Wiklund 2010), but why it happens is not yet known. However, association of virulence with colony morphology has been found in many pathogenic bacteria (Simpson et al 1987;van der Woude and Bäumler 2004). To survive and respond to changing environments requires bacteria to change their cell surface structures, which in the laboratory often leads to the formation of different colony morphologies (McCarter 1999;Avery 2006;Hilton et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Virulent and nonvirulent Flavobacterium columnare colony morphologies: characterization of chondroitin AC lyase activity and adhesion to polystyrene

Kunttu

Jokinen

Valtonen

et al. 2011

Journal of Applied Microbiology

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Aims: Colony morphology variants of fish pathogenic Flavobacterium columnare were studied to clarify the role of colony morphology change in the virulence of the bacterium. Typical rhizoid colony (Rz) variants are virulent and moderately adherent, nonrhizoid rough (R) colony variants are nonvirulent and highly adherent, and soft colony (S) variants are nonvirulent and poorly adherent. Methods and Results: Chondroitin AC lyase activity, adhesion to polystyrene at different temperatures and after modification of bacterial surface, and lipopolysaccharide (LPS) profiles of the variants were studied. The chondroitinase activity was significantly higher in the virulent, rhizoid variants than in the rough variants of the same strain. Temperature significantly increased the adhesion of rhizoid variants up to 20°C. Modification of bacterial surface suggested that adhesion molecules contain both carbohydrates and proteins. LPS did not differ between the variants of the same strain. Conclusions: The results suggest that in Fl. columnare both rhizoid colony morphology and high chondroitinase activity are needed for virulence and that temperature may promote the adhesion of the virulent variants to surfaces at fish farms. Significance and Impact of the Study: New information is produced on the virulence mechanisms of Fl. columnare and the reasons behind the survival of the bacterium at fish farms.

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“…The marine pathogenic V. vulnificus strain C7184(T) has been described by Simpson et al [10]. The marine Vibrio sp.…”

Section: Organisms Cultivation Starvation and Stress Conditionsmentioning

confidence: 99%

The induction of stress proteins in three marine Vibrio during carbon starvation

Holmquist

Jouper-Jaan

Weichart

et al. 1993

FEMS Microbiology Ecology

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The induction of DnaK and GroEL homologous proteins by heat‐shock and long‐term carbon starvation was studied in Vibrio vulnificus, Vibrio sp. strain S14, and Vibrio sp. strain DW1. In each Vibrio strain one protein (60 kDa) reacted with antibodies against Escherichia coli‐GroEL and two proteins, DnaK (69 kDa) and Sis1 (62‐60 kDa), reacted with antibodies against E. coli‐Dnak. The carbon starvation elicited induction of the stress proteins was strain‐specific, suggesting that the induction of stress proteins like DnaK and GroEL in marine Vibrios might not be a uniform starvation response. It appears as of these proteins, only DnaK in Vibrio sp. strain S14 remains induced after long‐term carbon starvation in the three marine bacterial strains that were tested.

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Correlation between virulence and colony morphology in Vibrio vulnificus

Cited by 191 publications

References 24 publications

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood

Virulent and nonvirulent Flavobacterium columnare colony morphologies: characterization of chondroitin AC lyase activity and adhesion to polystyrene

The induction of stress proteins in three marine Vibrio during carbon starvation

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