Correlation of Apoptosis with Comet Formation Induced by Tea Polyphenols in Human Leukemia Cells

Kundu, Trina; Bhattacharya, R.K.; Siddiqi, Maqsood; Roy, Madhumita

doi:10.1615/jenvpathtoxoncol.v24.i2.50

Cited by 9 publications

(10 citation statements)

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“…The SGCE technique has been used in numerous in vitro investigations to study antioxidant effects of individual compounds and complex mixtures in stable cell lines and in human lymphocytes. Typical examples are investigations of flavonoids by Anderson and coworkers with blood and sperm cells ( 280 , 281 ) , experiments concerning antioxidant effects of coffee and its constituents ( 282 ) , studies on the protective effects of tea catechins ( 283 , 284 ) to name only a few. Also quite a substantial number of in vivo studies with laboratory animals have been published, e.g.…”

Section: Conventional and New Methods For The Detection Of Dietary Anmentioning

confidence: 99%

Use of conventional and -omics based methods for health claims of dietary antioxidants: a critical overview

et al. 2008

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This article describes the principles and limitations of methods used to investigate reactive oxygen species (ROS) protective properties of dietary constituents and is aimed at providing a better understanding of the requirements for science based health claims of antioxidant (AO) effects of foods. A number of currently used biochemical measurements aimed of determining the total antioxidant capacity and oxidised lipids and proteins are carried out under unphysiologcial conditions and are prone to artefact formation. Probably the most reliable approaches are measurements of isoprostanes as a parameter of lipid peroxidation and determination of oxidative DNA damage. Also the design of the experimental models has a strong impact on the reliability of AO studies: the common strategy is the identification of AO by in vitro screening with cell lines. This approach is based on the assumption that protection towards ROS is due to scavenging, but recent findings indicate that activation of transcription factors which regulate genes involved in antioxidant defence plays a key role in the mode of action of AO. These processes are not adequately represented in cell lines. Another shortcoming of in vitro experiments is that AO are metabolised in vivo and that most cell lines are lacking enzymes which catalyse these reactions. Compounds with large molecular configurations (chlorophylls, anthocyans and polyphenolics) are potent AO in vitro, but weak or no effects were observed in animal/human studies with realistic doses as they are poorly absorbed. The development of -omics approaches will improve the scientific basis for health claims. The evaluation of results from microarray and proteomics studies shows that it is not possible to establish a general signature of alterations of transcription and protein patterns by AO. However, it was shown that alterations of gene expression and protein levels caused by experimentally induced oxidative stress and ROS related diseases can be normalised by dietary AO.

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Section: Conventional and New Methods For The Detection Of Dietary Anmentioning

confidence: 99%

Use of conventional and -omics based methods for health claims of dietary antioxidants: a critical overview

et al. 2008

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“…The intrinsic, via caspase 9, and extrinsic, via caspase 8, apoptotic pathways converge on the downstream effector caspase 3 (Rathmell and Thompson, 1999). Capase 3 was found to play an important role in DNA damage via MN and comet assays (Decordier et al, 2008, Kundu et al, 2005. In this study, BAPP was found to upregulate the activation of caspases 3, 8, and 9, and this demonstrated the signal transduction pathway of BAPP-induced apoptosis in HGFs.…”

Section: Discussionmentioning

confidence: 50%

The effects of cytotoxicity and genotoxicity induced by 2,2-bis[4-(acryloxypropoxy)phenyl]propane via caspases in human gingival fibroblasts

Yang

2012

Toxicol Ind Health

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The toxicity of dental materials has raised public concern over the past years. One of the most commonly used methacrylic monomers for building the three-dimensional structure of the dental resin composites is 2,2-bis[4-(acryloxypropoxy)phenyl]propane (BAPP). The purpose of this study is to evaluate the potential toxicological implication of BAPP on human gingival fibroblasts (HGFs). Flow cytometric, fluorometric, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assays were used to detect the mode of cell death, caspase activities, and cell viability, respectively. In addition, alkaline single-cell gel electrophoresis (COMET) and cytokinesis block micronucleus (MN) assays were applied to evaluate the genotoxicity. According to the results BAPP demonstrated a cytotoxic effect on HGFs in a dose- and time-dependent manner. With increasing concentrations of BAPP, the mode of cell death shifted from apoptosis to necrosis, and the activities of caspases 3, 8, and 9 were also significantly induced. Moreover, a dose-related increase in the number of micronucleus and DNA strand breaks hinted at the expression of genotoxicity by BAPP. In conclusion, the results gathered from this study had demonstrated that BAPP-induced cytotoxicity and genotoxicity on HGFs were mediated by DNA damage and the activation of caspases 3, 8, and 9.

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“…An explanation of the ability of oxidative stress to cause DNA damage is that it triggers a series of metabolic events within the cell that lead to the activation of nucleases, which cleave the DNA backbone. Recently, there have been many debates concerning the hypothesis that oxidative stress can cause rises in intracellular free calcium that might break DNA by activating calcium-dependent endonucleases in a mechanism similar to apoptosis ( − ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antiherpetic Activity and Genotoxic Effects of Tea Catechin Derivatives

Savi

Barardi

Simões

2006

J. Agric. Food Chem.

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This study evaluated the antiherpetic activity and genotoxicity of catechin and some of its derivatives using the MTT colorimetric and comet assays, respectively. The results showed that all compounds have antiviral activity with selective indices varying from 1.3 to 13, depending on the tested HSV-1 strain. It was observed that the same concentration of the compounds that protects the Vero cells against the viral infection induces genotoxicity. It was also observed that the molecules containing three hydroxyl groups on the B ring caused less DNA damage and showed better antiviral effect than those with two hydroxyls on the same ring, but if there is an additional galloyl group, these results can be altered. The bioavailability and stereochemistry could be related to the antiviral and genotoxic effects detected.

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Correlation of Apoptosis with Comet Formation Induced by Tea Polyphenols in Human Leukemia Cells

Cited by 9 publications

References 0 publications

Use of conventional and -omics based methods for health claims of dietary antioxidants: a critical overview

Use of conventional and -omics based methods for health claims of dietary antioxidants: a critical overview

The effects of cytotoxicity and genotoxicity induced by 2,2-bis[4-(acryloxypropoxy)phenyl]propane via caspases in human gingival fibroblasts

Evaluation of Antiherpetic Activity and Genotoxic Effects of Tea Catechin Derivatives

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