Correlation of collagen fibril properties and inner limiting membrane thickness with vitreoretinal adhesion in human eyes

Creveling, Christopher J.; Alsanea, Y.; Coats, Brittany

doi:10.1016/j.exer.2022.109189

Cited by 5 publications

(1 citation statement)

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“…The optimal value for each parameter was determined by varying the parameter on a test image followed by visual review of the fiber segments drawn on the image by the plugin preview function. The tabular output from the ridge detection consisted of variable length fiber segments which were stitched together using a custom MATLAB script developed by Creveling et al [13]. This script removes duplicate fibers and determines fiber orientation and width.…”

Section: Quantitative Image Analysismentioning

confidence: 99%

Confocal reflectance microscopy for mapping collagen fiber organization in the vitreous gel of the eye

Hwang

Morgan

Sun

et al. 2022

Preprint

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Vitreous collagen structure plays an important role in ocular mechanics, but capturing this structure with existing vitreous imaging methods is hindered by loss of sample position and orientation, low resolution, or small field of view. The objective of this study was to evaluate confocal reflectance microscopy as a solution to these limitations. The use of intrinsic reflectance avoids staining and optical sectioning eliminates the requirement for thin sectioning, minimizing processing for optimal preservation of natural structure. We developed a sample preparation and imaging strategy using ex vivo grossly sectioned porcine eyes. Imaging revealed a network of uniform diameter crossing fibers (1.1 plus/minus 0.3 micro metres for a typical image) with generally poor alignment (alignment coefficient = 0.40 plus/minus 0.21 for a typical image). To test the utility of our approach for detecting differences in fiber spatial distribution, we imaged eyes every 1 mm along an anterior-posterior axis originating at the limbus and quantified the number of fibers in each image. Fiber density was higher anteriorly near the vitreous base, regardless of imaging plane. These data demonstrate that confocal reflectance microscopy addresses the previously unmet need for a robust, micron-scale technique to map features of collagen networks in situ across the vitreous.

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Section: Quantitative Image Analysismentioning

confidence: 99%