Correlation of CTCs with disease progression in Indian oral cancer patients.

Abstract

e15541 Background: Head and neck squamous cell carcinoma is leading cancer in the India with Oral squamous cell carcinoma (OSCC) as the most frequent subtype. OSCC is classified as a locoregional disease and its increased frequency is attributed to lack of good biomarkers compared to other epithelial cancers. At the time of diagnosis, above 50% of cases present the manifestation of advanced-stage disease, and are predisposed to disease failure in spite of appropriate treatment. Thus, early diagnosis of OSCC can significantly reduce the disease burden. Here we describe regulatory approved method to establish Circulating tumor cells (CTCs) presence in OSCC Indian patients and its positive correlation with various clinicopathological parameters, suggesting the potential use of CTCs as a significant parameter to stratify oral cancer with respect to the disease advancement. Methods: In a cross-sectional observational study, 230 OSCC patients at the different pathological stage of the disease and treatment mode were enrolled. CTCs were isolated using approved OncoDiscover liquid biopsy technology (Drug controller general of India approved), platform technology based on immunomagnetic CTC enumeration. CTCs were detected for CK18 presence and well-defined, DAPI-stained nuclei. Enumerated CTC subsequently analyzed for various clinic-pathological parameters such as pstage, extra-capsular spread (ECS), lymphovascular emboli (LVE), perineural invasion (PNI) and depth of invasion (DOI). CTC cut off values were obtained to differentiate early vs advanced stages with respect to different clinical stages and parameters. Results: CTCs of OSCC patients correlated positively with the cancer stages (clinical as well as pathological) as well as aggressive pathological features. The presence of aggressive pathological features that often suggest the poor outcome of the disease, we observed a 25-50 % increase in CTC number. Early stage, treatment naïve patients had lower number of CTCs. Mean CTC number in advanced-stage patients was 50 % higher than early-stage OSCC patients. Conclusions: Considering a positive correlation of CTC number with various pathophysiological features, CTC can be contemplated as a reliable parameter to predict the disease outcome in oral cancer. The consistent presence of CTC across all disease stages also suggests a probable nature of OSCC as a biological systematic disease. Clinical trial information: CTRI/2018/03/012905.

Personalised Precision Medicine - A Novel Approach for Oral Cancer Management

“…CTC might be considered a trustworthy measure to predict the disease outcome in oral cancer due to a positive connection of CTC number with numerous pathophysiological characteristics. The presence of CTC at all stages of the disease shows that OSCC is most likely a biologically systematic disease [72].…”

Antibody mediated cotton-archetypal substrate for enumeration of circulating tumor cells and chemotherapy outcome in 3D tumors

“…1 The ability to conduct CTC isolation, detection, and downstream analysis, in a minimally invasive manner, provides a crucial tool for gathering diagnostic, prognostic, and therapeutic information on the status of a particular cancer disease. 2,3 Despite the immense prognostic value of CTCs, as demonstrated by several large clinical studies, [4][5][6] the isolation, expansion, and subsequent functional profiling of CTCs is challenging. However, in recent years, crucial advances have been made by several groups to successfully enrich these rare cells for downstream analysis.…”

“…Current clinically relevant CTC isolation methods validated by regulatory authorities (e.g. FDA-approved CellSearch®, 15 DCG(I)-approved OncoDiscover®, 6,[16][17][18][19] etc.) include a CTC enrichment step followed by a subsequent detection stage.…”