2001
DOI: 10.1002/ajh.1074
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Correlation of hematopoietic progenitor cell count determined by the SE‐9000™ automated hematology analyzer with CD34+ cell count by flow cytometry in leukapheresis products

Abstract: The yield of stem cell collection after mobilization is crucial for autologous peripheral blood stem cell (PBSC) transplantation. Quantitative determinations of CD34 + cells using flow cytometry or stem cell culture have been used, but these methods require much time, technical experience, and expensive reagents.

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Cited by 29 publications
(44 citation statements)
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“…Clinical implications for peripheral blood progenitor cells collection where the decision to commence apheresis is modified by peripheral blood WBC, MNC, or HPC ture forms to be counted (19,26,27). Previous studies reported a strong correlation between PB HPC and PB CD34+ cell counts (25,28), and we have reported a strong correlation between HPC enumerated by Sysmex SE-9000 and CD34+ cells in the leukapheresis products from data on 32 patients (20). This strong correlation led us to investigate the clinical usefulness of HPC as a screening test to decide when to commence PBPC collection.…”
Section: Discussionmentioning
confidence: 91%
See 1 more Smart Citation
“…Clinical implications for peripheral blood progenitor cells collection where the decision to commence apheresis is modified by peripheral blood WBC, MNC, or HPC ture forms to be counted (19,26,27). Previous studies reported a strong correlation between PB HPC and PB CD34+ cell counts (25,28), and we have reported a strong correlation between HPC enumerated by Sysmex SE-9000 and CD34+ cells in the leukapheresis products from data on 32 patients (20). This strong correlation led us to investigate the clinical usefulness of HPC as a screening test to decide when to commence PBPC collection.…”
Section: Discussionmentioning
confidence: 91%
“…We have previously reported on the preliminary data, which included 32 patients, of the correlation between the number of PB HPC and collected CD34+ cells (20). In the present study, we evaluated potential usefulness of PB HPC count in the clinical practice through the analysis of the data on 160 consecutive patients.…”
Section: ×10mentioning
confidence: 99%
“…Subsequently, it has been shown that mononuclear cell counts correlate poorly with CD34 þ cells; hence, the true number of infused stem cells in early studies is not well known. 18,19 At the time of earlier studies, fluoroquinolone antibiotic prophylaxis was not available. Ciprofloxacin was not approved until 1987, and triazole antifungal antibiotics, such as ketoconazole and fluconazole, were not introduced until 1990.…”
Section: Discussionmentioning
confidence: 99%
“…The quantities of CD34 þ cells in PB and leukapheresis components were determined as described previously. 11,12 High-dose chemotherapy with PBPC support…”
Section: MC Pbpc Harvest and Cd34 Cell Quantitationmentioning
confidence: 99%
“…In AMC, from July 1998 to July 2002, the first PBPC harvest was initiated on the day when the first of the following occurred: (1) WBC count exceeded 10.0 Â 10 9 /l; (2) MNC count exceeded 1.0 Â 10 9 /l; or (3) hematopoietic progenitor cell (HPC) count, as assessed by an automated hematology analyzer (SE-9000, Sysmex, Kobe, Japan), exceeded 5/ ml. 11,12 Since July 2002, apheresis was started only if the peripheral blood (PB) HPC count exceeded 5/ml. 13 In YUMC, PBPC collection was started when PB CD34 þ cell count exceeded 10/ml.…”
Section: MC Pbpc Harvest and Cd34 Cell Quantitationmentioning
confidence: 99%