Correlation of histology and acoustic parameters of liver tissue on a microscopic scale

Steen, Antonius F.W. van der; Thijssen, J.M.; Laak, J.A.W.M. van der; Ebben, G.P.J.; Wilde, P.C.M. de

doi:10.1016/0301-5629(94)90082-5

Cited by 18 publications

(4 citation statements)

References 36 publications

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“…The largest cross-sectional area of each sample was selected and cut in 4 m thick sections that were stained with Masson trichrome. The percentage perimysial and endomysial tissue (for the larger part containing connective tissue but also blood vessels and nerve bundles) were measured for each muscle using digital image analysis as previously described (van der Steen et al 1994). Microscopic fields of vision were digitized using a RGB CCD camera, resulting in images measuring 11.7 mm 2 (specimen level pixel size 5.1 ϫ 5.1um 2 ).…”

Section: Muscle Histologymentioning

confidence: 99%

Skeletal Muscle Ultrasound: Correlation Between Fibrous Tissue and Echo Intensity

Pillen

Tak

Zwarts

et al. 2009

Ultrasound in Medicine & Biology

504

419

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Section: Muscle Histologymentioning

confidence: 99%

Skeletal Muscle Ultrasound: Correlation Between Fibrous Tissue and Echo Intensity

Pillen

Tak

Zwarts

et al. 2009

Ultrasound in Medicine & Biology

504

419

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“…[8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] In vitro attenuation measurement methods are difficult to transfer in vivo since most of the clinical studies can be performed only in a single-transducer backscatter configuration. Indeed, in vitro backscatter coefficient measurements are generally compensated with an estimation of the attenuation using transmission mode 8,9 or reflection mode with a reflector on the opposite side of the examined tissue, 10,11 which is difficult to implement in clinical practice. Many researchers have then attempted to improve the in vivo backscatter power spectrum estimates by assuming a priori attenuation values for the different intervening tissue layers.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound characterization of red blood cell aggregation with intervening attenuating tissue-mimicking phantoms

Franceschini

Destrempes

et al. 2010

The Journal of the Acoustical Society of America

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The analysis of the ultrasonic frequency-dependent backscatter coefficient of aggregating red blood cells reveals information about blood structural properties. The difficulty in applying this technique in vivo is due to the frequency-dependent attenuation caused by intervening tissue layers that distorts the spectral content of signals backscattered by blood. An optimization method is proposed to simultaneously estimate tissue attenuation and blood structure properties, and was termed the structure factor size and attenuation estimator ͑SFSAE͒. An ultrasound scanner equipped with a wide-band 25 MHz probe was used to insonify porcine blood sheared in both Couette and tubular flow devices. Since skin is one of the most attenuating tissue layers during in vivo scanning, four skin-mimicking phantoms with different attenuation coefficients were introduced between the transducer and the blood flow. The SFSAE gave estimates with relative errors below 25% for attenuations between 0.115 and 0.411 dB/ MHz and kR Ͻ 2.08 ͑k being the wave number and R the aggregate radius͒. The SFSAE can be useful to examine in vivo and in situ abnormal blood conditions suspected to promote pathophysiological cardiovascular consequences.

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“…After fixation, the aortas were stained with Sudan IV (Sigma Chemical Co) to identify lipid-containing atherosclerotic plaques. The area covered by atherosclerotic lesions was quantified with a computer-assisted planimetry system (Kontro-Vidas plus system 16 ) coupled to a video camera (Sony). Repeated measurements of the area covered by atherosclerotic plaques, performed in a blinded fashion by a skilled technician, resulted in an intra-assay coefficient of variation for this system of <5%.…”

Section: Extent Of Aortic Atherosclerosismentioning

confidence: 99%

Comparative study on the effect of low-dose vitamin E and probucol on the susceptibility of LDL to oxidation and the progression of atherosclerosis in Watanabe heritable hyperlipidemic rabbits.

Kleinveld

Demacker

Stalenhoef

1994

Arterioscler Thromb

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The diet of Watanabe heritable hyperlipidemic (WHHL) rabbits was supplemented with a low dose (0.025% [wt/wt]) of the antioxidant vitamin E or probucol. The effect of 6 months of treatment on the susceptibility of low-density lipoproteins (LDLs) to oxidative modification and on established atherosclerotic lesions was studied. Vitamin E administration had no effect on plasma lipid levels; probucol supplementation decreased plasma total cholesterol. Vitamin E levels in plasma and LDL increased threefold in the course of treatment with this antioxidant. Six months of treatment with vitamin E and probucol increased the lag time of conjugateddiene formation of LDL subjected to in vitro oxidation by 54% (P<.00l) and 51% (P=.O19), respectively. In this LDL-oxidizability assay, only vitamin E reduced the maximal rate of conjugated-diene production (-24%, P<.001). Neither vita- showing that probucol attenuates the progression of atherosclerotic lesion formation in Watanabe heritable hyperlipidemic (WHHL) rabbits, an animal model for familial hypercholesterolemia, provided strong evidence that lipid peroxidation plays a prominent role in atherogenesis. On the basis of these studies and others, 35 a lipid peroxidation hypothesis has been developed to explain the process of atherogenesis in humans.6 Central in this hypothesis is the oxidation of low-density lipoproteins (LDLs), leading to characteristic physicochemical modifications. Once LDL is modified, unlimited amounts are taken up through the scavenger receptor of macrophages present in the arterial intima. The excessive accumulation of cholesterol in macrophages results in their transformation to foam cells, the hallmark of atherosclerotic lesion formation.LDL contains several natural antioxidants that provide protection against excessive oxidative modification.

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Correlation of histology and acoustic parameters of liver tissue on a microscopic scale

Cited by 18 publications

References 36 publications

Skeletal Muscle Ultrasound: Correlation Between Fibrous Tissue and Echo Intensity

Skeletal Muscle Ultrasound: Correlation Between Fibrous Tissue and Echo Intensity

Ultrasound characterization of red blood cell aggregation with intervening attenuating tissue-mimicking phantoms

Comparative study on the effect of low-dose vitamin E and probucol on the susceptibility of LDL to oxidation and the progression of atherosclerosis in Watanabe heritable hyperlipidemic rabbits.

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