Correlation of intracellular oxygen and cell metabolism by simultaneous PLIM of phosphorescent TLD1433 and FLIM of NAD(P)H

Kalinina, Sviatlana; Breymayer, Jasmin; Reess, Kirsten; Lilge, Lothar; Mandel, Arkady; Rück, Angelika

doi:10.1002/jbio.201800085

Cited by 29 publications

(32 citation statements)

References 55 publications

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“…The emission spectrum of TLD1433 under two-photon excitation was measured in solution as well as within living cells and presented previously. 4,25 Similar to other ruthenium (II) complexes investigated by us, the tris-(2,2 0 -bipyridyl) dichloride (Ru (bpy) 2þ 3 ) and cHSA-PEO-TPP-Ru, TLD1433 emits in the range from 550 nm and 670 nm. 20,26 We were able to perform two-photon microscopy using the 250 MHz Ti:Sa on a Zeiss LSM 710 NLO.…”

Section: Resultssupporting

confidence: 53%

“…3 Within this study, investigations where done with a 250 MHz repetition rate laser and compared with 80 MHz excitation. As will be discussed within this paper, PLIM imaging could be very well achieved at 250 MHz excitation rate, however, simultaneous FLIM¯tting procedures, recently published with 80 MHz excitation rate, 4 need further modi¯cations in the detection electronics (see Sec. 2).…”

Section: Introductionmentioning

confidence: 99%

“…Recently published results could show that oxygen is the main quencher of TLD1433 leading to a shortening of the phosphorescence lifetime. 4 PLIM results reported in this paper were reached by two photon excitations of the drug with a femtosecond (fs) pulsed Ti:Sapphire laser working at 80 MHz repetition rate and time-correlated single photon counting (TCSPC) detection electronics. As discussed above, the interesting question now was whether it is possible to follow up oxygen concentration and consumption by PLIM of TLD1433 using higher repetition rates.…”

Section: Introductionmentioning

confidence: 99%

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Fast repetition rate fs pulsed lasers for advanced PLIM microscopy

Kalinina

Jelzow

Pl€otzing³

et al. 2019

J. Innov. Opt. Health Sci.

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Simultaneous metabolic and oxygen imaging is promising to follow up therapy response, disease development and to determine prognostic factors. FLIM of metabolic coenzymes is now widely accepted to be the most reliable method to determine cellular bioenergetics. Also, oxygen consumption has to be taken into account to understand treatment responses. The phosphorescence lifetime of oxygen sensors is able to indicate local oxygen changes. For phosphorescence lifetime imaging (PLIM) dyes based on ruthenium (II) coordination complexes are useful, in detail TLD1433 which possesses a variety of different triplet states, enables complex photochemistry and redox reactions. PLIM is usually reached by two photon excitation of the drug with a femtosecond (fs) pulsed Ti:Sapphire laser working at 80[Formula: see text]MHz repetition rate and (time-correlated single photon counting) (TCSPC) detection electronics. The interesting question was whether it is possible to follow up PLIM using faster repetition rates. Faster repetition rates could be advantageous for the induction of specific photochemical reactions because of similar light doses used normally in standard CW light treatments. For this, a default 2[Formula: see text]-FLIM–PLIM system was expanded by adding a second fs pulsed laser (“helixx”) which provides 50[Formula: see text]fs pulses at a repetition rate of 250[Formula: see text]MHz, more than 2.3[Formula: see text]W average power and tunable from 720[Formula: see text]nm to 920[Formula: see text]nm. The laser beam was coupled into the AOM instead of the default 80[Formula: see text]MHz laser. We demonstrated successful applications of the 250[Formula: see text]MHz laser for PLIM which correlates well with measurements done by excitation with the conventional 80[Formula: see text]MHz laser source.

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Section: Resultssupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fast repetition rate fs pulsed lasers for advanced PLIM microscopy

Kalinina

Jelzow

Pl€otzing³

et al. 2019

J. Innov. Opt. Health Sci.

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“…The mechanism of this PS is still being elucidated, but a recent study indicated that the complex may localize in the endosomal or lysosomal organelles. 95 Ruthenium(II) complexes can undergo receptor-mediated transport into cells by association with transferrin; hence, in a study, complex 99 was premixed with transferrin to allow for the formation of Rutherrin before administration into AY27 cells and subcutaneous CT26.CL25 tumor mice models. 96 Rutherrin was found to improve cellular uptake and maintain the structural integrity to produce an increase in the defining PDT effect of complex 99.…”

Section: ■ Therapeutic Luminescent Complexesmentioning

confidence: 99%

Luminescent Ruthenium(II) Polypyridine Complexes for a Wide Variety of Biomolecular and Cellular Applications

2019

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Ruthenium(II) polypyridine complexes are one of the most extensively studied and developed systems in the family of luminescent transition-metal complexes. Notably, there has been a large amount of interest in the biological applications of these luminescent ruthenium(II) complexes because of their rich photophysical and photochemical properties. In this Viewpoint, we explore past and recent works on the possible biological and cellular applications of these promising complexes, with a focus on their use as bioimaging reagents, biomolecular probes, and phototherapeutic agents.

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“…Cell localisation of TLD-1433 and its impact on cell metabolism by changing the cellular redox balance was published in a recent study. 24 Cyclometalated Ru(II) complexes are usually more photostable and their absorption spectra is red-shifted compared to diamine Ru(II) complexes. The (photo-)toxicity of the complexes was checked in two cell lines, namely SK-MEL-28 (melanoma) and CCD-1064Sk (normal skin fibroblasts).…”

Section: Figure 2 Tld-1411 and Tld-1433mentioning

confidence: 99%