Correlation of Metabolic and Chemiluminescent Responses of Granulocytes from Three Female Siblings with Chronic Granulomatous Disease

Allen, Robert C.; Stjernholm, R.; Reed, Margaret A.; Harper, Thomas B.; Gupta, Shanti S.; Steele, Richard; Waring, William W.

doi:10.1093/infdis/136.4.510

Cited by 46 publications

(11 citation statements)

References 33 publications

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“…Another rarely applied parameter to define phagocyte activity is the initial slope of the stimulation curve (ALLEN et al, 1977;STEVENS et al, 1978). We took the maximal increase between two determination points of the ascending loop of the stimulation curve as a percentage of the maximal CL.…”

Section: Discussionmentioning

confidence: 99%

Comparative Chemiluminescence Studies with elicited Peritoneal and Spleen Cells of different Strains of Mice and Rats and the Guinea pig

Müller‐Peddinghaus¹,

G²,

Schumacher³

1983

Zentralblatt für Veterinärmedizin Reihe B

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Summary Chemiluminescence (CL) was used to measure the activity and responsiveness of phagocytes from nine strains of mice, two rat strains and the guinea pig. The release of oxygen radicals was determined by the luminol‐amplified CL method, with thioglycollate‐elicited peritoneal exudate cells (PEC) harvested 24 and 96 hours thereafter and spleen cells (SC) from untreated animals. To define species and strains, different parameters were employed. Unseparated cell populations were stimulated by zymosan particles opsonized with human complement. Murine 24 h PEC, mainly neutrophilic granulocytes, have a 14 times higher spontaneous CL than those from the guinea pig or rat. The 24 h PEC of the latter and SC from CBA/J mice exhibited very high stimulation indices above 50. The mean stimulation index of 74 for murine SC is remarkably high compared with 12 for 96 h PEC and 13 for 24 h PEC. The mean integral CL values of the 24 h PEC are 14 times higher than for the 96 h PEC and 3.5 times higher than for the SC. The guinea pig has a low maximal increase of the stimulation curve of 96 h PEC and reaches peak values later than rats and mice for 24 h and 96 h PEC. Inbred Lewis/Iv rats have considerably higher stimulation indices for 24 h and 96 h PEC than outbred Sprague Dawley rats. A good response by 96 h PEC was seen in DBA/2J mice. A low increase of the ascending loop of the stimulation curve characterizes C 57 BL/6‐bg mice. BALB/c‐nu mice exhibit overall a good cellular response. CBA/J mice are a low responder strain. Zusammenfassung Vergleichende Chemilumineszenzuntersuchungen mit induzierten Peritoneal‐ und Milzzellen verschiedener Mäuse‐ und Rattenstämme und beim Meerschweinchen Mittels Chemilumineszenz (CL) wird die Aktivität bzw. Stimulierbarkeit von Phagozyten neun verschiedener Mäusestämme, zweier Rattenstämme und vom Meerschweinchen bestimmt. Die Freisetzung von Sauerstoffradikalen wird nach der Luminol‐abhängigen Chemilumineszenzmethode bestimmt. Verwendet werden sowohl Thioglykollat‐induzierte Peritonealzellen (PEC), die 24 oder 96 Stunden später geerntet werden, als auch Milzzellen (SC) unbehandelter Tiere. Die gemischten Zellpräparationen werden mit durch Humankomplement opsonisierte Zymosanpartikel stimuliert. Überwiegend aus neutroph. Granulozyten bestehende 24 h PEC der Maus haben eine 14fach stärkere spontane CL als Meerschweinchen oder Ratte. Der mittlere Stimulationsindex von SC der Maus beträgt 74 im Vergleich zu 12 für 96 h PEC und 13 für 24 h PEC. Der mittlere Integralwert aktivierter 24 h PEC ist 14fach größer als der von 96 h PEC und 3,5fach höher als der von SC. Phagozyten des Meerschweinchens erreichen den Gipfelpunkt der Zellaktivität später als die von Maus und Ratte. Der Stimulationsindex von 24 h und 96 h PEC der Lewis/Iv‐Ratte ist deutlich höher als bei Sprague Dawley‐Ratten. DBA/2J‐ und BALB/c‐nu‐Mäuse weisen eine gute, C 57 BL/6‐bg‐ und CBA/J‐Mäuse eine schlechte Zellantwort auf. Résumé Chemiluminescence comparée avec des cellules péritonéales et de la rate de différentes souches de souri...

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Section: Discussionmentioning

confidence: 99%

Comparative Chemiluminescence Studies with elicited Peritoneal and Spleen Cells of different Strains of Mice and Rats and the Guinea pig

Müller‐Peddinghaus¹,

G²,

Schumacher³

1983

Zentralblatt für Veterinärmedizin Reihe B

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“…Recently, however, we have demonstrated that increased levels of indomethacin-sensitive suppressor function are directly correlated with increased conversion of arachidonic acid to prostaglandins of the E series on the part of peripheral blood glass-adherent cells [8]. There is a substantial body of evidence which suggests that CL provides a reliable, quantifiable assay of oxidative metabolism in reticuloendothelial cells [2,4,5,6]. Macrophage activation has been assessed using CL [30], and human monocytes also respond with light emission to a variety of stimuli [20,25].…”

Section: Discussionmentioning

confidence: 99%

Chemiluminescence, suppression and cytotoxicity in peripheral blood mononuclear cells from solid tumor cancer patients

Boer

1982

Cancer Immunol Immunother

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Chemiluminescence, indomethacin-sensitive suppression, and adherent cell cytotoxicity were measured in peripheral blood mononuclear cells (PBMC) from normal subjects and solid tumor cancer patients. These functions were found to be differentially affected by malignant disease. In cancer patients with disseminated disease, indomethacin-sensitive suppression and chemiluminescence emission were increased to a level significantly higher than normal without a concurrent increase in adherent cell cytotoxic function. In cancer patients with at most minimum residual diseases, the levels of chemiluminescence, indomethacin-sensitive suppression, and adherent cell cytotoxicity found were comparable to those of the normal study population. In vitro stimulation of cells from patients with disseminated disease by phorbol myristic acetate (PMA) increased chemiluminescence overcame the suppressive effects of indomethacin-sensitive suppressor cells, and increased adherent cell cytotoxicity; in cells from patients with at most minimum residual disease, PMA increased chemiluminescence and cytotoxicity without influencing the activity of indomethacin-sensitive suppressor cells. Vaccination of lung cancer patients with Freund's complete adjuvant or Freund's complete adjuvant plus tumor antigen extracts led to increased levels of chemiluminescence and increased levels of adherent cell cytotoxicity without altering indomethacin-sensitive regulatory cell function.

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“…There is a substandial body of evidence which suggests that CL provides a reliable, quantifiable assay of oxidative metabolism in reticuloendothelial cells [1,3,4,6], and with appropriate modifications, can also be used to measure particle uptake [12] or serum opsonic activity [1,2]. Macrophage activation has been assessed by means of CL [25], and since human monocytes also respond with light emission to a variety of stimuli [16,20], it is reasonable to suggest that the enhanced CL from the PBMC of patients with advanced cancer reflects an in vivo stimulation, although CL alone cannot provide any direct information on the nature of the stimulus.…”

Section: Discussionmentioning

confidence: 99%

Chemiluminescence in peripheral blood mononuclear cells of solid tumor cancer patients

Braun

Harris

Maximovich

et al. 1981

Cancer Immunol Immunother

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Correlation of Metabolic and Chemiluminescent Responses of Granulocytes from Three Female Siblings with Chronic Granulomatous Disease

Cited by 46 publications

References 33 publications

Comparative Chemiluminescence Studies with elicited Peritoneal and Spleen Cells of different Strains of Mice and Rats and the Guinea pig

Comparative Chemiluminescence Studies with elicited Peritoneal and Spleen Cells of different Strains of Mice and Rats and the Guinea pig

Chemiluminescence, suppression and cytotoxicity in peripheral blood mononuclear cells from solid tumor cancer patients

Chemiluminescence in peripheral blood mononuclear cells of solid tumor cancer patients

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