Correlation of quantified contrast-enhanced power Doppler ultrasonography with immunofluorescent analysis of microvessel density in spontaneous canine tumours

Abstract

Conventionally, tumour vascularity is assessed invasively by immunofluorescent analysis. Quantified contrast-enhanced power Doppler ultrasound has been used to measure tumour angiogenesis non-invasively in humans and experimental animals. The purpose of this study was to correlate quantified contrast-enhanced power Doppler ultrasound with immunofluorescent results in 45 spontaneous canine tumours. With power Doppler, mean vascularity was high in squamous cell carcinomas, moderate in malignant oral melanomas and low in sarcomas. There was high mean vascularity in squamous cell carcinomas and low mean vascularity in sarcomas and malignant oral melanomas. Although Doppler parameters correlated moderately with microvascular density for all tumours (P = 0.004, r = 0.4), they did not correlate within histology groups. These analyses show that vascularity differs among canine tumour histology groups. However, dependent on the method used, measurement of tumour vascularity can provide different biological information.

Correlation of Pretreatment Polarographically Measured Oxygen Pressures with Quantified Contrast-Enhanced Power Doppler Ultrasonography in Spontaneous Canine Tumors and their Impact on Outcome After Radiation Therapy

“…Median vascularity and blood volume differed significantly between histology groups: squamous cell carcinomas were highly vascularized and perfused, whereas in malignant melanomas mean vascularity and blood volume were moderate and in sarcomas they were low. These results confirm recently published data from our group [33]. Fibrosarcoma was found to have the lowest tissue oxygen pressure with median values below the prognostically relevant described limit of ≤ 10 mmHg [2,36], and the high and moderate vascularization and perfusion of squamous cell carcinomas and malignant melanoma, respectively, resulted in only moderate amounts of hypoxic regions.…”

“…For contrast-enhanced power Doppler ultrasonography, settings as described in Ohlerth et al [33] were used. A region of interest (ROI) was drawn around the tumor boundaries and two measurements were computed for each ROI.…”

Computed Tomographic Features of Basihyoid Ectopic Thyroid Carcinoma in Dogs

“…Main differential diagnoses of a hyoid‐located mass are soft tissue sarcomas, primary bone tumors, or primary laryngeal carcinomas infiltrating the surrounding hyoid region. Soft tissue sarcomas (except hemangiosarcomas) and condrosarcomas are typically low‐enhancing lesions and therefore a mild, homogeneous enhancement is expected in contrast‐enhanced CT . Primary laryngeal carcinomas originate from the mucosa that covers the inner laryngeal surface and a severe invasion of the lumen can occur very early with this type of neoplasia .…”

Characterization of tumor vasculature distributions in central and peripheral regions based on Doppler ultrasound

“…4). 34,41 The spatial difference between tumor perfusion and vascular density 11 days after tumor inoculation (Figs. 5 and 6) indicates that angiogenesis is generally more rapid in the tumor periphery than in the tumor center region.…”