Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies

Tseng, Scheffer C.G.; Järvinen, Mikko; Nelson, William G.; Huang, Jason; Woodcock‐Mitchell, Janet; Sun, Tung Tien

doi:10.1016/0092-8674(82)90234-3

Cited by 609 publications

(297 citation statements)

References 46 publications

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“…A detailed catalogue of septin expression would be particularly useful in combination with a definition of the various combinations of septin hetero-oligomers that exist under physiological conditions. A parallel might be drawn here with the significant insights that similar catalogues gave in the keratin field [132,133].…”

Section: Septin Expression Profilesmentioning

confidence: 99%

The pathobiology of the septin gene family

Hall

Russell

2004

The Journal of Pathology

288

283

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Septins are an evolutionarily conserved group of GTP-binding and filament-forming proteins that belong to the large superclass of P-loop GTPases. While originally discovered in yeast as cell division cycle mutants with cytokinesis defects, they are now known to have diverse cellular roles which include polarity determination, cytoskeletal reorganization, membrane dynamics, vesicle trafficking, and exocytosis. Septin proteins form homo-and heterooligomeric polymers which can assemble into higher-order filaments. They are also known to interact with components of the cytoskeleton, ie actin and tubulin. The precise role of GTP binding is not clear but a current model suggests that it is associated with conformational changes which alter binding to other proteins. There are at least 12 human septin genes, and although information on expression patterns is limited, most undergo complex alternative splicing with some degree of tissue specificity. Nevertheless, an increasing body of data implicates the septin family in the pathogenesis of diverse disease states including neoplasia, neurodegenerative conditions, and infections. Here the known biochemical properties of mammalian septins are reviewed in the light of the data from yeast and other model organisms. The data implicating septins in human disease are considered and a model linking these data is proposed. It is posited that septins can act as regulatable scaffolds where the stoichiometry of septin associations, modifications, GTP status, and the interactions with other proteins allow the regulation of key cellular processes including polarity determination. Derangements of such septin scaffolds thus explain the role of septins in disease states.

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Section: Septin Expression Profilesmentioning

confidence: 99%

The pathobiology of the septin gene family

Hall

Russell

2004

The Journal of Pathology

288

283

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“…The synthesis of cytokeratins is usually maintained during malignant transformation, and this feature serves as one of the hallmarks of epithelium-derived tumours (Tseng et al, 1982), including tumours of the urinary tract (Moll et al, 1982(Moll et al, , 1988Letocha et al, 1993;Cilento et al, 1994). Cytokeratin 7 has been considered a urothelial marker (Cilento et al, 1994), while cytokeratin 13 is a squamous cell marker with trace amounts of component 13 expressed in basal and intermediate cells of human urothelium and low-grade TCC (Moll et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a novel transplantable orthotopic rat bladder transitional cell tumour model

et al. 1999

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Summary An animal tumour model that mimics the human counterpart is essential for preclinical evaluation of new treatment modalities. The objective of this study was to develop and characterize such a model. To accomplish this, the established AY-27 rat bladder transitional cell carcinoma (TCC) cell line was transplanted orthotopically into Fischer CDF344 female rats. AY-27 TCC cells were grown in monolayer cell culture and instilled intravesically as single cell suspensions into bladders that had been conditioned with mild acid washing. Tumour growth was assessed weekly by subjecting the rats to magnetic resonance imaging (MRI). At intervals following implantation and MRI tumour detection, the animals were sacrificed for necropsy, histological examination and immunocytochemical studies. Flow cytometry was also performed for detection of Fas or Fas-ligand expression on AY-27 cells. The overall tumour establishment was 95% (97/102 rats) at 12-50 days, while in a subgroup of animals sacrificed at 16 days, 80 out of 82 animals (97%) developed TCC, the majority of which was superficial. Tumour stage was assessed by gross pathology and light microscopy. Histological examination of the tumour specimens confirmed the presence of grade II-III TCC. Immunocytochemistry confirmed that the tumour model maintained the features of TCC. The changes seen on MRI correlated well with the extent of tumour invasion identified histologically. Patchy carcinoma in situ could be detected histologically 12-13 days post-inoculation, and progressed to papillary tumour or invasive disease thereafter. Neither Fas nor Fas-ligand was expressed on AY-27 cells. The orthotopic AY-27 TCC model is highly reproducible and is ideal for preclinical studies on experimental intravesical therapies.

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“…1 Pancytokeratin clone AE1/AE3 is a cocktail of 2 monoclonal antibodies that identifies most human cytokeratins 30 and thus may be used to identify cells of simple and stratified epithelial origin. [28][29][30] Antibody AE1 immunoreacts with an antigenic determinant present on most of subfamily A cytokeratins, including cytokeratins (CK) 10, 13, 14, 15, 16, and 19 (from 56.5 to 40 kD). 28 Antibody AE3 reacts with an antigenic determinant shared by the subfamily B cytokeratins, including CK 1, 2, 3, 4, 5, 6, 7, and 8 (from 65 to 52 kD).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Characterization of Mammary Squamous Cell Carcinoma of the Dog

et al. 2008

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Abstract. Squamous cell carcinoma of the mammary gland is rare in both veterinary and human medicine. Whereas human metaplastic and squamous variants are known, the objectives of the current study were to ascertain the presence of such entities in canine mammary tumors and to distinguish them from other (epidermal, sweat gland) squamous tumors that may develop in the same area. A panel of antibodies (anticytokeratin [CK] 19, CK 14, CK 5/6, pancytokeratin, and vimentin) was used on 18 mammary gland malignancies with squamous features and 16 malignant skin tumors (11 squamous cell carcinomas of the skin and 5 sweat glands). Fifteen of the 18 mammary carcinomas were classified as metaplastic carcinomas, and the remaining 3 were classified as squamous cell carcinomas. The 2 most useful markers to establish the histogenesis of mammary tumors were pancytokeratin and CK 19. All other antibodies were equally expressed (CK 14 and 5/6) in all histotypes. The antibody panel discriminated primary epidermal squamous tumors (pancytokeratin positive and CK 19 negative) from gland-derived squamous neoplasms (pancytokeratin positive and CK 19 positive) but failed to distinguish primary mammary tumors from other squamous tumors of glandular origin.

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Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies

Cited by 609 publications

References 46 publications

The pathobiology of the septin gene family

The pathobiology of the septin gene family

Characterization of a novel transplantable orthotopic rat bladder transitional cell tumour model

Immunohistochemical Characterization of Mammary Squamous Cell Carcinoma of the Dog

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